US3888741A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Culturing means and test kit
Assignee: Bayer Corp
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Filing: 1974-04-25
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Granted: 1975-06-10
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Expires: 1994-04-25
Abstract
Abstract Means for culturing microorganisms in an environment enriched with a particular gas is disclosed. The culturing means comprises a microbiological nutrient means capable of supporting microbiological growth and a composition for producing controlled release of the particular gas. A test kit for presumptive identification of microorganisms is also provided comprising the above described culturing means and an indicator for the microorganism being detected. Such culturing means and test kit are particularly useful in culturing and identifying Neisseria gonorrhoeae.
Inventors
- 👤 Ronald Freake
- 👤 Mau H Kuo
- 👤 Devendra Vaikunthlal Mehta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 19:11
CPC Classifications
C; C12; C12M; C12M41/00; C12M41/30; C12M41/34; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
56 words
MEANS FOR CULTURING A MICROORGANISM IN AN ENVIRONMENT ENRICHED WITH A GAS, COMPRISING A MICROBIOLOGICAL NUTRIENT MEANS; A GAS GENERATING COMPOSITION COMPRISING AT LEAST TWO SOLID REACTANTS WHICH WHEN CONTACTED WITH ATER REACT TO PRODUCE SAID GAS AND MEANS FOR CONTROLLING THE RATE OF REACTION BETWEEN SAID REACTANTS, AND THEREBY THE RATE OF RELEASE OF SAID
Claim 2
Dependent Claim
56 words
MEANS FOR CULTURING A MICROORGANISM IN AN ENVIRONMENT ENRICHED WITH A GAS, COMPRISING A MICROBIOLOGICAL NUTRIENT MEANS; A GAS GENERATING COMPOSITION COMPRISING AT LEAST TWO SOLID REACTANTS WHICH WHEN CONTACTED WITH ATER REACT TO PRODUCE SAID GAS AND MEANS FOR CONTROLLING THE RATE OF REACTION BETWEEN SAID REACTANTS, AND THEREBY THE RATE OF RELEASE OF SAID
Claim 3
Dependent Claim
28 words
Culturing means as in claim 1 wherein said means for controlling said reaction rate comprises, in combination, a water soluble polymeric substance and a water insoluble polymeric substance.
Claim 4
Dependent Claim
23 words
Culturing means as in claim 2 wherein said water soluble polymeric substance is hydroxypropyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulsoe, polyacrylamide, or polyvinyl alcohol.
Claim 5
Dependent Claim
27 words
Culturing means as in claim 2 wherein said water insoluble polymeric substance is cellulose acetate, cellulose nitrate, cellulose acetate butyrate, ethyl cellulose, butyl cellulose, polybutadiene, or polystyrene.
Claim 6
Dependent Claim
22 words
Culturing means as in claim 1 wherein said nutrient means comprises a bibulous carrier member having a microbiological nutrient substance incorporated therewith.
Claim 7
Dependent Claim
18 words
Culturing means as in claim 1 additionally comprising a base member by which said nutrient means is carried.
Claim 8
Dependent Claim
17 words
Culturing means as in claim 6 wherein said gas generating composition is carried by said enclosing means.
Claim 9
Dependent Claim
22 words
Culturing means as in claim 6 wherein said nutrient means comprises a bibulous carrier member having a microbiological nutrient substance incorporated therewith.
Claim 10
Dependent Claim
18 words
Culturing means as in claim 8 wherein said gas generating composition is incorporated with a bibulous carrier member.
Claim 11
Dependent Claim
18 words
Culturing means as in claim 6 wherein said enclosing means is an envelope for enclosing said base member.
Claim 12
Dependent Claim
18 words
Culturing means as in claim 6 wherein said gas generating composition is also carried by said base member.
Claim 13
Dependent Claim
57 words
A test kit for the presumptive identification of a particular variety of microorganism comprising the culturing means of claim 11 and an indicator for said particular variety of microorganism, said indicator being incorporated with a bibulous carrier member, said bibulous carrier member being carried by said base member and being movable into contact with said nutrient means.
Claim 14
Dependent Claim
25 words
Culturing means as in claim 6 wherein said enclosing means comprises a cover member cooperable with said base member to form a closed chamber therewith.
Claim 15
Dependent Claim
18 words
Culturing means as in claim 13 wherein said gas generating composition is also carried by said base member.
Claim 16
Dependent Claim
53 words
Culturing means as in claim 14 wherein said base member is formed with at least two wells, said nutrient means being disposed in one of said wells and said gas generating composition being disposed in another of said wells, said wells being in gaseous communication when said base and cover members are engaged.
Claim 17
Dependent Claim
22 words
Culturing means as in claim 15 wherein said nutrient means comprises a bibulous carrier member having a microbiological nutrient substance incorporated therewith.
Claim 18
Dependent Claim
22 words
A test kit for the presumptive identification of Neisseria gonorrhoeae comprising the culturing means of claim 16 and a cytochrome oxidase indicator.
Claim 19
Dependent Claim
19 words
Culturing means as in claim 16 wherein said gas generating composition is in the form of a solid matrix.
Claim 20
Dependent Claim
18 words
Culturing means as in claim 16 wherein said gas generating composition is incorporated with a bibulous carrier member.
Claim 21
Dependent Claim
18 words
Culturing means as in claim 16 wherein said nutrient means is selective for a particular variety of microorganism.
Claim 22
Dependent Claim
17 words
Culturing means as in claim 13 wherein said gas generating composition is carried by said cover member.
Claim 23
Dependent Claim
23 words
Culturing means as in claim 1 wherein said solid reactants comprise at least two substances which when contacted with water produce carbon dioxide.
Claim 24
Dependent Claim
18 words
Culturing means as in claim 22 wherein said solid reactants comprise an organic acid and an inorganic carbonate.
Claim 25
Dependent Claim
26 words
Culturing means as in claim 1 for selectively culturing a particular variety of microorganism wherein said nutrient means is selective for said particular variety of microorganism.
Claim 26
Dependent Claim
29 words
A test kit for the presumptive identification of a particular variety of microorganism comprising the culturing means of claim 1 and an indicator for said particular variety of microorganism.
Claim 27
Dependent Claim
19 words
A test kit as in claim 25 wherein said nutrient means is selective for said particular variety of microorganism.
Claim 28
Dependent Claim
17 words
A test kit as in claim 25 wherein said indicator is incorporated with a bibulous carrier member.
Claim 29
Dependent Claim
25 words
A test kit as in claim 25 wherein said particular variety of microorganism is Neisseria gonorrhoeae and wherein said indicator is a cytochrome oxidase indicator.
Claim 30
Dependent Claim
16 words
A test kit as in claim 28 wherein said nutrient means is selective for Neisseria gonorrhoeae.
Claim 31
Dependent Claim
28 words
A test kit as in claim 28 wherein said cytochrome oxidase indicator is N,N,N'',N''-tetramethyl-p-phenylenediamine dihydrochloride, p-aminodimethylaniline, dimethyl phenylenediamine, dimethyl-p-phenylenediamine oxalate, or a mixture of dimethyl phenylenediamine and alpha-naphthol.
Claim 32
Dependent Claim
119 words
Culturing means for selectively culturing Neisseria gonorrhoeae in an environment enriched with carbon dioxide, which device comprises a base member having bottom and sidewalls and formed with at least two wells; a bibulous carrier member disposed in one of said wells and having a nutrient medium selective for Neisseria gonorrhoeae incorporated therewith; a carbon dioxide generating composition disposed in another of said wells, said carbon dioxide generating composition comprising a water soluble polymeric substance, a water insoluble polymeric substance, and at least two solid reactants which in the presence of water react to produce carbon dioxide; and removable cover means engagable with said sidewall to form with said base member a closed chamber providing gaseous communication between said wells.
Claim 33
Dependent Claim
119 words
Culturing means for selectively culturing Neisseria gonorrhoeae in an environment enriched with carbon dioxide, which device comprises a base member having bottom and sidewalls and formed with at least two wells; a bibulous carrier member disposed in one of said wells and having a nutrient medium selective for Neisseria gonorrhoeae incorporated therewith; a carbon dioxide generating composition disposed in another of said wells, said carbon dioxide generating composition comprising a water soluble polymeric substance, a water insoluble polymeric substance, and at least two solid reactants which in the presence of water react to produce carbon dioxide; and removable cover means engagable with said sidewall to form with said base member a closed chamber providing gaseous communication between said wells.
Claim 34
Dependent Claim
18 words
Culturing means as in claim 31 wherein said solid reactants comprise an organic acid and an inorganic carbonate.
Claim 35
Dependent Claim
23 words
Culturing means as in claim 31 wherein said water soluble polymeric substance is hydroxypropyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, polyaCrylamide, or polyvinyl alcohol.
Claim 36
Dependent Claim
27 words
Culturing means as in claim 31 wherein said water insoluble polymeric substance is cellulose acetate, cellulose nitrate, cellulose acteate butyrate, ethyl cellulose, butyl cellulose, polybutadiene, or polystyrene.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-06-09 | 1978-12-09 | 1979-06-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-06-09 | 1982-12-09 | 1983-06-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-06-09 | 1986-12-09 | 1987-06-10 | $7700.00 | Window Closed / Lapsed |