Mucopolysaccharides, their preparation and use in cosmetic and pharmaceutical compositions

Claim 1

A method for producing mucopolysaccharides from a material selected from the group consisting of cutaneous teguments, umbilical cords and mixtures thereof from the foetus of an animal selected from the group consisting of a cow and a sheep comprising under aseptic conditions: a. grinding said material, b. extracting said ground material with an ionic extraction solution having a pH between 1.5 and 9 and present in amounts of about 1-20 ml/g of said ground material, said ionic extraction solution being selected from the group consisting of i. a 0.1-2.5 M solution of a neutral salt selected from the group consisting of sodium chloride and potassium chloride, ii. a 0.1 M solution of an acid salt selected from the group consisting of sodium acid phosphate, potassium acid phosphate, disodium citrate, dipotassium citrate and mixtures thereof, iii. a solution of hydrochloric acid having a pH of about 1.5 and iv. a 1N solution of sodium hydroxide, c. digesting said extracted, ground material in the presence of a proteolytic enzyme selected from the group consisting of trypsin, ficin, pepsin, pronase, papain and pancreatin, said enzyme being present in amounts effective to digest said material, said digesting being continued until said material becomes fluid, thereby forming a broth, d. adding to said broth a solvent selected from the group consisting of acetone, methanol, ethanol and isopropanol in amounts effective to precipitate said mucopolysaccharides therefrom, e. separating said precipitated mucopolysaccharides by filtration, decantation or centrifugation, f. washing said separated mucopolysaccharides, and g. drying said mucopolysaccharides thereby providing mucopolysaccharides in the form of a white solid, soluble in water and physiological serum solution and having a molecular weight ranging between about 5,000-2,500,000.

Claim 2

A method for producing mucopolysaccharides from a material selected from the group consisting of cutaneous teguments, umbilical cords and mixtures thereof from the foetus of an animal selected from the group consisting of a cow and a sheep comprising under aseptic conditions: a. grinding said material, b. extracting said ground material with an ionic extraction solution having a pH between 1.5 and 9 and present in amounts of about 1-20 ml/g of said ground material, said ionic extraction solution being selected from the group consisting of i. a 0.1-2.5 M solution of a neutral salt selected from the group consisting of sodium chloride and potassium chloride, ii. a 0.1 M solution of an acid salt selected from the group consisting of sodium acid phosphate, potassium acid phosphate, disodium citrate, dipotassium citrate and mixtures thereof, iii. a solution of hydrochloric acid having a pH of about 1.5 and iv. a 1N solution of sodium hydroxide, c. digesting said extracted, ground material in the presence of a proteolytic enzyme selected from the group consisting of trypsin, ficin, pepsin, pronase, papain and pancreatin, said enzyme being present in amounts effective to digest said material, said digesting being continued until said material becomes fluid, thereby forming a broth, d. adding to said broth a solvent selected from the group consisting of acetone, methanol, ethanol and isopropanol in amounts effective to precipitate said mucopolysaccharides therefrom, e. separating said precipitated mucopolysaccharides by filtration, decantation or centrifugation, f. washing said separated mucopolysaccharides, and g. drying said mucopolysaccharides thereby providing mucopolysaccharides in the form of a white solid, soluble in water and physiological serum solution and having a molecular weight ranging between about 5,000-2,500,000.

Claim 3

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

Claim 4

A METHOD FOR PRODUCING MUCOPOLYSACCHARIDES FROM A MATERIAL SELECTED FROM THE GROUP CONSISTING OF CUTANEOUS TEGUMENTS, UMBILICAL CORDS AND MIXTURES THEREOF FROM THE FOETUS OF AN ANIMAL SELECTED FROM THE GROUP CONSISTING OF A COW AND A SHEEP COMPRISING UNDER ASEPTIC CONDITIONSL: A. GRINDING SAID MATERIAL, B. EXTRACTING SAID GROUND MATERIAL WITH AN IONIC EXTRACTION SOLUTION HAVING A PH BETWEEN 1.5 AND 9 AND PRESENT IN AMOUNTS OF ABOUT 1-20 ML/G OF SAID GROUND MATERIAL, SAID IONIC EXTRACTION SOLUTION BEING SELECTED FROM THE GROUP CONSISTING OF I. A 0.1-2.5 M SOLUTION OF A NEUTRAL SALT SELECTED FROM THE GROUP CONSISTING OF SODIUM CHLORIDE AND POTASSIUM CHLORIDE, II. A 0.1 M SOLUTION OF AN ACID SALT SELECTED FROM THE GROUP CONSISTING OF SODIUM ACID PHOSPHATE, POTASSIUM ACID PHOSPHATE, DISODIUM CITRATE, DIPOTASSIUM CITRATE AND MIXTURES THEREOF, III. A SOLUTION OF HYDROCHLORIC ACID HAVING A PH OF ABOUT 1.5 AND IV. A 1N SOLUTION OF SODIUM HYDROXIDE, C. DIGESTING SAID EXTRACTED, GROUND MATERIAL IN THE PRESENCE OF A PROTEOLYTIC ENZYME SELECTED FROM THE GROUP CONSISTING OF TRYPSIN, FICIN, PEPSIN, PRONASE, PAPAIN AND PANCREATIN, SAID ENZYME BEING PRESENT IN AMOUNTS EFFECTIVE TO DIGEST SAID MATERIAL, SAID DIGESTING BEING CONTINUED UNTIL SAID MATERIAL BECOMES FLUID, THEREBY FORMING A BROTH, D. ADDING TO SAID BROTH A SOLVENT SELECTED FROM THE GROUP CONSISTING OF ACETONE, METHANOL, ETHANOL AND ISOPROPANOL IN AMOUNTS EFFECTIVE TO PRECIPITATE SAID MUCOPOLYSACCHARIDES THEREFROM, E. SEPARATING SAID PRECIPITATED MUCOPOLYSACCHARIDES BY FILTRATION, DECANTATION OR CENTRIFUGATION, F. WASHING SAID SEPARATED MUCOPOLYSACCHARIDES, AND G. DRYING SAID MUCOPOLYSACCHARIDES THEREBY PROVIDING MUCOPOLYSACCHARIDES IN THE FORM OF A WHITE SOLID, SOLUBLE IN WATER AND PHYSIOLOGICAL SERUM SOLUTION AD HAVING A MOLECULAR WEIGHT RANGING BETWEEN ABOUT 5,000-2,500,000.

Claim 17

A method for producing a mucopolysaccharide from a material selected from the group consisting of cutaneous teguments, umbilical cords and their mixtures from the foetus of an animal selected from the group consisting of a cow and a sheep comprising under aseptic conditions a. grinding said material, b. extracting said ground material with an ionic extraction solution having a pH between 1.5 and 9 and present in amounts of about 1-20 ml/g of said ground material, said ionic extraction solution being selected from the group consisting of i. a 0.1-2.5 M solution of a neutral salt selected from the group consisting of sodium chloride and potassium chloride, ii. a 0.1 M solution of an acid salt selected from the group consisting of sodium acid phosphate, potassium acid phosphate, disodium citrate, dipotassium citrate and mixtures thereof, iii. a solution of hydrochloric acid having a pH of about 1.5 and iv. a 1N solution of sodium hydroxide, c. digesting said extracted, ground material in the presence of a proteolytic enzyme selected from the group consisting of trypsin, ficin, pepsin, pronase, papain and pancreatin, said enzyme being present in amounts effective to digest said material, said digesting being continued until said material becomes fluid, thereby forming a broth, d. adding to said broth a complexing agent selected from the group consisting of cetylpyridinium chloride and cetyltrimethylammonium bromide in amounts effective to form a complex of said mucopolysaccharides therewith thereby precipitating said mucopolysaccharide complex from said broth, e. adding to said precipitated mucopolysaccharide complex a 1-4 M solution of a salt selected from the group consisting of sodium chloride, potassium chloride and sodium sulfate to dissociate said mucopolysaccharide from said complexing agent, f. dialyzing said saline solution to produce a dialyzate consisting essentially of said mucopolysaccharides essentially free from said saline solution and said complexing agent and g. lyophilizing said mucopolysaccharides thereby providing a mucopolysaccharide in the form of a white solid, soluble in water and physiological serum solution and having a molecular weight ranging between about 5,000-2,500,000.

Claim 18

A method for producing a mucopolysaccharide from a material selected from the group consisting of cutaneous teguments, umbilical cords and their mixtures from the foetus of an animal selected from the group consisting of a cow and a sheep comprising under aseptic conditions a. grinding said material, b. extracting said ground material with an ionic extraction solution having a pH between 1.5 and 9 and present in amounts of about 1-20 ml/g of said ground material, said ionic extraction solution being selected from the group consisting of i. a 0.1-2.5 M solution of a neutral salt selected from the group consisting of sodium chloride and potassium chloride, ii. a 0.1 M solution of an acid salt selected from the group consisting of sodium acid phosphate, potassium acid phosphate, disodium citrate, dipotassium citrate and mixtures thereof, iii. a solution of hydrochloric acid having a pH of about 1.5 and iv. a 1N solution of sodium hydroxide, c. digesting said extracted, ground material in the presence of a proteolytic enzyme selected from the group consisting of trypsin, ficin, pepsin, pronase, papain and pancreatin, said enzyme being present in amounts effective to digest said material, said digesting being continued until said material becomes fluid, thereby forming a broth, d. adding to said broth a complexing agent selected from the group consisting of cetylpyridinium chloride and cetyltrimethylammonium bromide in amounts effective to form a complex of said mucopolysaccharides therewith thereby precipitating said mucopolysaccharide complex from said broth, e. adding to said precipitated mucopolysaccharide complex a 1-4 M solution of a salt selected from the group consisting of sodium chloride, potassium chloride and sodium sulfate to dissociate said mucopolysaccharide from said complexing agent, f. dialyzing said saline solution to produce a dialyzate consisting essentially of said mucopolysaccharides essentially free from said saline solution and said complexing agent and g. lyophilizing said mucopolysaccharides thereby providing a mucopolysaccharide in the form of a white solid, soluble in water and physiological serum solution and having a molecular weight ranging between about 5,000-2,500,000.

Claim 31

A method for producing a mucopolysaccharide from a material selected from the group consisting of cutaneous teguments, umbilical cords and their mixtures from the foetus of an animal selected from the group consisting of a cow and a sheep comprising under aseptic conditions a. grinding said material, b. extracting said ground material with an ionic extraction solution having a pH between 1.5 And 9 and present in amounts of about 1-20 ml/g of said ground material, said ionic extraction solution being selected from the group consisting of i. a 0.1-2.5 M solution of a neutral salt selected from the group consisting of sodium chloride and potassium chloride, ii. a 0.1 M solution of an acid salt selected from the group consisting of sodium acid phosphate, potassium acid phosphate, disodium citrate, dipotassium citrate and mixtures thereof, iii. a solution of hydrochloric acid having a pH of about 1.5 and iv. a 1N solution of sodium hydroxide, c. digesting said extracted, ground material in the presence of a proteolytic enzyme selected from the group consisting of trypsin, ficin, pepsin, pronase, papain and pancreatin, said enzyme being present in amounts effective to digest said material, said digesting being continued until said material becomes fluid, thereby forming a broth, d. adding to said broth a protein precipitating agent selected from the group consiting of perchloric acid and trichloroacetic acid in amounts such that the concentration of the acid in the respective resulting mixture is between about 5-10 weight percent, e. removing the proteins from the supernactant liquid, f. dialyzing against water said supernactant liquid and g. lyophilizing the dialyzate thereby providing a mucopolysaccharide in the form of a white solid, soluble in water and physiological serum solutions and having a molecular weight ranging between about 5,000-2,500,000.

Claim 32

A method for producing a mucopolysaccharide from a material selected from the group consisting of cutaneous teguments, umbilical cords and their mixtures from the foetus of an animal selected from the group consisting of a cow and a sheep comprising under aseptic conditions a. grinding said material, b. extracting said ground material with an ionic extraction solution having a pH between 1.5 And 9 and present in amounts of about 1-20 ml/g of said ground material, said ionic extraction solution being selected from the group consisting of i. a 0.1-2.5 M solution of a neutral salt selected from the group consisting of sodium chloride and potassium chloride, ii. a 0.1 M solution of an acid salt selected from the group consisting of sodium acid phosphate, potassium acid phosphate, disodium citrate, dipotassium citrate and mixtures thereof, iii. a solution of hydrochloric acid having a pH of about 1.5 and iv. a 1N solution of sodium hydroxide, c. digesting said extracted, ground material in the presence of a proteolytic enzyme selected from the group consisting of trypsin, ficin, pepsin, pronase, papain and pancreatin, said enzyme being present in amounts effective to digest said material, said digesting being continued until said material becomes fluid, thereby forming a broth, d. adding to said broth a protein precipitating agent selected from the group consiting of perchloric acid and trichloroacetic acid in amounts such that the concentration of the acid in the respective resulting mixture is between about 5-10 weight percent, e. removing the proteins from the supernactant liquid, f. dialyzing against water said supernactant liquid and g. lyophilizing the dialyzate thereby providing a mucopolysaccharide in the form of a white solid, soluble in water and physiological serum solutions and having a molecular weight ranging between about 5,000-2,500,000.