US3876775A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stable intravenously injectable plasma protein free from hypotensive effects and process for its production
Assignee: Bayer Corp
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Filing: 1973-06-13
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Granted: 1975-04-08
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Expires: 1993-06-13
Abstract
Abstract The depressor effect observed when heat-treated plasma protein fractions are rapidly infused is eliminated by separating, e.g., with a surface active adsorbent or cation exchanger, ultrafiltration membrane or by gel molecular sieve, a low molecular weight depressor substance having a molecular weight of less than 10,000 from the plasma proteins.
Inventors
- 👤 Kenichi Izaka
- 👤 Eizo Tsutsui
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 09:53
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/04; A61K38/08; C; C07; C07K; C07K14/00; C07K14/435; C07K14/76; C07K14/765
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
23 words
A STERILE AQUEOUS SOLUTION OF AN ELECTROPHORETICALLY NONHOMOGENOUS, HUMAN, HEAT-STABLE PLASMA PROTEIN FRACTION SUITABLE FOR RAPID INTRAVENOUS INFUSION, SUBSTANTIALLY FREE OF DEPR3ESSOR EFFECTS.
Claim 2
Dependent Claim
23 words
A STERILE AQUEOUS SOLUTION OF AN ELECTROPHORETICALLY NONHOMOGENOUS, HUMAN, HEAT-STABLE PLASMA PROTEIN FRACTION SUITABLE FOR RAPID INTRAVENOUS INFUSION, SUBSTANTIALLY FREE OF DEPR3ESSOR EFFECTS.
Claim 3
Dependent Claim
21 words
The solution of claim 1, stabilized with a stabilizer selected from the group consisting of sodium acetyltryptophanate, N-acetyltryptophan and sodium caprylate.
Claim 4
Dependent Claim
13 words
The solution of claim 1 wherein the protein concentration is about 5 percent.
Claim 5
Dependent Claim
26 words
The solution of claim 3 wherein the protein comprises at least 83 percent albumin and no more than 17 percent of alpha globulin and beta globulin.
Claim 6
Dependent Claim
27 words
The solution of claim 1 wherein the plasma protein fraction consists essentially of a mixture of 85-92 percent albumin, 4-10 percent globulin and 2-7 percent beta globulin.
Claim 7
Dependent Claim
13 words
The solution according to claim 5, heat-treated until free of infective hepatitis virus.
Claim 8
Dependent Claim
88 words
A process for the production of an electrophoretically non-homogeneous plasma protein solution which is substantially free of depressor substance so that the solution may be rapidly infused intravenously without causing a significant fall in blood pressure, which comprises the step of contacting a solution of an electrophoretically non-homogeneous human plasma protein containing depressor substance with a material selected from the group consisting of surface active adsorbents, cation exchangers, ultrafiltration membranes and gel filtration molecular sieves which removes the depressor substance from the protein. membranes and gel molecular sieves.
Claim 9
Dependent Claim
88 words
A process for the production of an electrophoretically non-homogeneous plasma protein solution which is substantially free of depressor substance so that the solution may be rapidly infused intravenously without causing a significant fall in blood pressure, which comprises the step of contacting a solution of an electrophoretically non-homogeneous human plasma protein containing depressor substance with a material selected from the group consisting of surface active adsorbents, cation exchangers, ultrafiltration membranes and gel filtration molecular sieves which removes the depressor substance from the protein. membranes and gel molecular sieves.
Claim 10
Dependent Claim
32 words
The process of claim 7 wherein the proteins of the starting protein solution are a mixture consisting of at least 83 percent albumin and not more than 17 percent alpha-globulin and beta-globulin.
Claim 11
Dependent Claim
28 words
The process of claim 8 comprising the step of heating the starting stable protein solution prior to contacting the protein solution with the material which removes the depressor.
Claim 12
Dependent Claim
17 words
The process of claim 9 wherein the heating step is conducted for about 1 to 4 hours.
Claim 13
Dependent Claim
41 words
The process of claim 10 comprising the step of heating the protein solution after the removal of the depressor therefrom at about 60.degree. C. for a period of time of at least 10 hours effective to destroy any hepatitis virus therein.
Claim 14
Dependent Claim
28 words
The process of claim 7 wherein the depressor is removed by contacting the starting solution of non-homogeneous human plasma protein containing a depressor with a surface active adsorbent.
Claim 15
Dependent Claim
23 words
The process of claim 12 wherein the surface active adsorbent is selected from the group consisting of silica gel and aluminum hydroxide gel.
Claim 16
Dependent Claim
32 words
The process of claim 13 wherein the starting plasma solution containing the depressor is a heat-treated mixture of at least 83 percent albumin and not more than 17 percent alpha-globulin and beta-globulin.
Claim 17
Dependent Claim
22 words
The process of claim 13 wherein the surface active adsorbent and the solution containing the depressor are mixed in a batchwise operation.
Claim 18
Dependent Claim
23 words
The process of claim 13 wherein the plasma solution containing the depressor substance is passed through a column of the surface active adsorbent.
Claim 19
Dependent Claim
22 words
The process of claim 15 further including the step of separating the surface active adsorbent from the mixture by centrifugation or filtration.
Claim 20
Dependent Claim
27 words
The process of claim 7 wherein the depressor is removed by contacting the starting solution of non-homogeneous human plasma protein containing a depressor with a cation exchanger.
Claim 21
Dependent Claim
31 words
The process of claim 18 wherein the cation exchanger is a member of the group consisting of sulfonated polystyrene cross-linked with divinylbenzene, carboxymethyl cellulose, and cross-linked dextran having terminal carboxymethyl groups.
Claim 22
Dependent Claim
28 words
The process of claim 7 wherein the depressor is removed by contacting the starting solution of non-homogeneous human plasma protein containing a depressor with gel filtration molecular sieve.
Claim 23
Dependent Claim
34 words
The process of claim 20 wherein the gel filtration particles are selected from the group consisting of cross-linked dextrans and agarose gels having the capacity for entrapping substances with molecular weights below about 10,000.
Claim 24
Dependent Claim
53 words
The process of claim 7 wherein the depressor is removed by filtering the starting solution of non-homogeneous human plasma protein containing a depressor with an ultrafiltration membrane which will allow the depressor substance to pass through the membrane into the filtrate but prevents the passage of substantially all of the desired plasma proteins.
Claim 25
Dependent Claim
52 words
The process of claim 7 which comprises the steps of a. heating a solution of a non-homogeneous stable human plasma protein fraction at about 60.degree. C., b. contacting the heated solution with a material selected from the group consisting of surface active adsorbents, cation exchangers, ultrafiltration membranes and gel filtration molecular sieves.
Claim 26
Dependent Claim
16 words
The process of claim 23 comprising the step of cooling the solution prior to step b).
Claim 27
Dependent Claim
16 words
The process of claim 24 comprising the step of separating any precipitate from the cooled solution.
Claim 28
Dependent Claim
32 words
The process of claim 25 comprising heating the solution in step a) for about 1 to 4 hours and again heating the cooled solution, for about 10 hours at about 60.degree. C.
Claim 29
Dependent Claim
28 words
The process of claim 26 wherein in Step b) the material is a surface active agent selected from the group consisting of silica gel and aluminum hydroxide gel.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-07 | 1978-10-07 | 1979-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-07 | 1982-10-07 | 1983-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-07 | 1986-10-07 | 1987-04-08 | $7700.00 | Window Closed / Lapsed |