US3873683A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Pregnancy test composition and method
Assignee: Princeton Laboratories Inc
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Filing: 1973-05-02
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Granted: 1975-03-25
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Expires: 1993-05-02
Abstract
Abstract There is provided a novel composition for the direct determination of the existence of pregnancy by the admixture therewith of a sample of urine, plasma, or serum from the female believed to be pregnant. This novel composition is prepared by mixing serum obtained from rabbits previously injected with human chorionic gonadotrophin (HCG), with polystyrene latex particles and heating for a short time.
Inventors
- 👤 Bernard Fishbein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 07:11
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/76; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/817; Y10S436/818
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
70 words
A REAGENT FOR THE DETECTION OF HUMAN CHORONIC GONADOTROPHIN WHICH CONSISTS ESSENTIALLY OF AN AQUEOUS SUSPENSION OF FINELY DIVIDED POLYSTYRENE LATEX PARTICLES HAVING ADSORBED THEREON HUMAN CHORIONIC GONADOTROPHIN ANTIBODIES, SAID REAGENT BEING PREPARED BY MIXING AN AQUEOUS SUSPENSION OF LATEX PARTICLES WITH WHOLE BLOOD HUMAN CHORIONIC GONADOTROPHIN ANTISERUM CONTAINING ANTI-HUMAN CHORIONIC GONADOTROPHIN AND HEATING SAID MIXTURE AT ABOUT 54* TO ABOUT 57*C. FOR FROM ABOUT 1 TO ABOUT 2 HOURS.
Claim 2
Dependent Claim
70 words
A REAGENT FOR THE DETECTION OF HUMAN CHORONIC GONADOTROPHIN WHICH CONSISTS ESSENTIALLY OF AN AQUEOUS SUSPENSION OF FINELY DIVIDED POLYSTYRENE LATEX PARTICLES HAVING ADSORBED THEREON HUMAN CHORIONIC GONADOTROPHIN ANTIBODIES, SAID REAGENT BEING PREPARED BY MIXING AN AQUEOUS SUSPENSION OF LATEX PARTICLES WITH WHOLE BLOOD HUMAN CHORIONIC GONADOTROPHIN ANTISERUM CONTAINING ANTI-HUMAN CHORIONIC GONADOTROPHIN AND HEATING SAID MIXTURE AT ABOUT 54* TO ABOUT 57*C. FOR FROM ABOUT 1 TO ABOUT 2 HOURS.
Claim 3
Dependent Claim
41 words
The reagent of claim 1, wherein the particles are polystyrene latex particles having a diameter of between 0.15 and 0.9 microns and wherein the concentration ratio by volume of latex particles to suspending medium is between 0.5 percent to 2 percent.
Claim 4
Dependent Claim
18 words
The reagent of claim 2, wherein said reagent is buffered to a pH of between 6 and 8.4.
Claim 5
Dependent Claim
52 words
The reagent of claim 2, additionally comprising a diluent selected from the group consisting of water and an aqueous buffer, wherein said reagent is prepared by the additional steps of adding the diluent, and reheating the diluted mixture at about 54* to about 57*C for from about 1 to about 2 hours.
Claim 6
Dependent Claim
80 words
A method of detection of human chorionic gonadotrophin in a specimen suspected of containing the same which comprises the steps of: a. mixing the reagent of claim 1 with an equal volume of specimen suspected to contain human chorionic gonadotrophin selected from the group consisting of serum and plasma; b. agitating the mixture; c. determining the presence of agglutination of particles of the reagent medium, the occurrence of said agglutination indicating the presence of human chorionic gonadotrophin in the specimen.
Claim 7
Dependent Claim
81 words
A method of detecting human chorionic gonadotrophin in a specimen of serum or plasma suspected of containing the same which comprises: a. mixing between 0.025 and 0.1 ml of the reagent of claim 2 with an equal volume of the specimen; b. agitating the mixture for from about 1 to about 3 minutes; c. and determining the presence of agglutination at the end of this time, the occurrence of said agglutination indicating the presence of human chorionic gonadotrophin in the specimen.
Claim 8
Dependent Claim
27 words
The method of claim 6, which comprises the additional step of diluting said specimen with an equal volume of buffer prior to addition of the latex reagent.
Claim 9
Independent Claim
107 words
A method for preparing a composition for the direct determination of the existence of pregnancy by the admixture therewith of a sample of urine, plasma or serum, which consists essentially of: a. obtaining blood containing anti-human chorionic gonadotrophin from lower mammals to which human chorionic gonadotrophin had been administered; b. separating the cells from the serum by centrifugation of the whole blood; c. mixing an aqueous suspension of polystyrene latex particles with the whole blood serum, wherein the concentration ratio by volume of latex particles to suspending medium is between 0.5% and 2%; and d. heating said mixture at about 54*-57*C. for about 1 to 2 hours.
Claim 10
Independent Claim
107 words
A method for preparing a composition for the direct determination of the existence of pregnancy by the admixture therewith of a sample of urine, plasma or serum, which consists essentially of: a. obtaining blood containing anti-human chorionic gonadotrophin from lower mammals to which human chorionic gonadotrophin had been administered; b. separating the cells from the serum by centrifugation of the whole blood; c. mixing an aqueous suspension of polystyrene latex particles with the whole blood serum, wherein the concentration ratio by volume of latex particles to suspending medium is between 0.5% and 2%; and d. heating said mixture at about 54*-57*C. for about 1 to 2 hours.
Claim 11
Dependent Claim
25 words
The mehtod of claim 8 wherein the polystyrene latex particles have a diameter of 0.15 to 1 micron and wherein the lower mammal is rabbit.
Claim 12
Dependent Claim
56 words
The method of claim 9 wherein the latex particles have a diameter of 0.6 to 0.9 micron and wherein said reagent is prepared by the additional steps of adding a diluent selected from the group consisting of water and an aqueous buffer, and reheating the diluted mixture at about 54*-57*C. for about 1 to 2 hours.
References Cited (Backward Citations)
US3088875A
Cited
US3551555A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-24 | 1978-09-23 | 1979-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-24 | 1982-09-23 | 1983-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-24 | 1986-09-23 | 1987-03-25 | $7700.00 | Window Closed / Lapsed |