US3873432A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Continuous preparative electrophoresis method
Assignee: Individual
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Filing: 1972-10-30
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Granted: 1975-03-25
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Expires: 1992-10-30
Abstract
Abstract Method for continually separating mixtures of proteins or other charged particles of differing size, and/or weight, such as those present in a serum, using the principle of electrophoresis. A multi-celled separating unit has portions of the mixture to be separated applied by an applicator sequentially to the several cells of the unit. Separate collection compartments for the respective separated components are located with relation to the separating unit so that each compartment continually receives a single one of the components as it is separated out by the separating cells.
Inventors
- 👤 Louis Israel
- 👤 Leslie Bernstein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 23:53
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
119 words
THE METHOD OF CONTINUOUTLY ELECTROPHORETICALLY SEPARATING AND COLLECTING THE INDIVIDUAL COMPONENTS OF A MIXTURE WHERE SUCH COMPONENTS HAVE DIFFERING ELECTROPHORETIC MOBILITY RATES, COMPRISING THE STEPS OF: A. DEPOSITING GIVEN AMOUNTS OF SAID MIXTURE IN PREDETERMINED SEQUENCE INTO THE INPUT ENDS OF A PLUTALITY OF ELECTROPHORETIC SEPARATING CELLS EACH ALSO HAVING AN OUTPUT END; B. APPYLING AND ELECTRICAL FIELD TO EACH OF SAID CELLS IN A DIRE CTION EXTENDING BETWEEN SAID INPUT AND OUTPUT ENDS; C.COLLCTING THE OUTPUTS OF SAID CELLS AT SAID OUTPUT ENDS IN SAID SAME PREDETERMINED SEQUENCE, WITH THE TIME INTERVALS BETWEEN THE DEPOSIT INTO EACH CELL AND THE COLLECTION FROM THAT SAME CELL BEING ALL SUBSTANTIALLY EQUAL AND CORRELATED TO THE PARTICULAR COMPONENT TO BE COLLECTED.
Claim 2
Independent Claim
119 words
THE METHOD OF CONTINUOUTLY ELECTROPHORETICALLY SEPARATING AND COLLECTING THE INDIVIDUAL COMPONENTS OF A MIXTURE WHERE SUCH COMPONENTS HAVE DIFFERING ELECTROPHORETIC MOBILITY RATES, COMPRISING THE STEPS OF: A. DEPOSITING GIVEN AMOUNTS OF SAID MIXTURE IN PREDETERMINED SEQUENCE INTO THE INPUT ENDS OF A PLUTALITY OF ELECTROPHORETIC SEPARATING CELLS EACH ALSO HAVING AN OUTPUT END; B. APPYLING AND ELECTRICAL FIELD TO EACH OF SAID CELLS IN A DIRE CTION EXTENDING BETWEEN SAID INPUT AND OUTPUT ENDS; C.COLLCTING THE OUTPUTS OF SAID CELLS AT SAID OUTPUT ENDS IN SAID SAME PREDETERMINED SEQUENCE, WITH THE TIME INTERVALS BETWEEN THE DEPOSIT INTO EACH CELL AND THE COLLECTION FROM THAT SAME CELL BEING ALL SUBSTANTIALLY EQUAL AND CORRELATED TO THE PARTICULAR COMPONENT TO BE COLLECTED.
Claim 3
Dependent Claim
49 words
The method of claim 1 including additionally similarly collecting said outputs in said same sequence at time intervals equal to one another but different from said first equal time intervals, to also separate and collect a different component of said mixture in the same sequence as said predetermined sequence.
Claim 4
Dependent Claim
17 words
The method of claim 2 in which the separating cells are moved and the collectors remain stationary.
Claim 5
Dependent Claim
24 words
The method of claim 2 in which the collectors and the point of application of the mixture move and the separating cells remain stationary.
Claim 6
Dependent Claim
25 words
The method of claim 1 further comprising accomplishing said collecting at a collecting station, and causing relative movement between said cells and said collecting station.
Claim 7
Dependent Claim
21 words
The method of claim 5 further comprising accomplishing said depositing at an input station having corresponding relative movement to said cells.
Claim 8
Dependent Claim
23 words
The method of claim 1 further comprising accomplishing said depoSiting and collecting at fixed locations adjacent said cell input and output ends respectively.
Claim 9
Independent Claim
159 words
The method of continuously electrophoretically separating and collecting a single component of a mixture having components with differing electrophoretic mobility rates, in an apparatus having a plurality of cells of an electrophoretic supporting medium, each cell having an input and an output, and said cells being arranged in an endless sequence in a closed loop, said apparatus also having an applicator for supplying said mixture to said cells and a collector for deriving said components from said cells, comprising the steps of a. sequentially and cyclically supplying said cells at their inputs at substantially fixed uniform time intervals with a mixture having components to be separated; b. continuously applying an electric field along said cells to cause electrophoretic separation of said components along said cells; and c. collecting material exiting from each said cell at its output sequentially and cyclically, each collection for a cell occurring at a substantially fixed predetermined time interval after the loading of that cell.
Claim 10
Independent Claim
159 words
The method of continuously electrophoretically separating and collecting a single component of a mixture having components with differing electrophoretic mobility rates, in an apparatus having a plurality of cells of an electrophoretic supporting medium, each cell having an input and an output, and said cells being arranged in an endless sequence in a closed loop, said apparatus also having an applicator for supplying said mixture to said cells and a collector for deriving said components from said cells, comprising the steps of a. sequentially and cyclically supplying said cells at their inputs at substantially fixed uniform time intervals with a mixture having components to be separated; b. continuously applying an electric field along said cells to cause electrophoretic separation of said components along said cells; and c. collecting material exiting from each said cell at its output sequentially and cyclically, each collection for a cell occurring at a substantially fixed predetermined time interval after the loading of that cell.
Claim 11
Dependent Claim
21 words
The method of claim 8 further comprising moving said cells relative to said applicator and said collector between said supplying steps.
Claim 12
Dependent Claim
16 words
The method of claim 9 further comprising accomplishing said supplying and said collecting at fixed positions.
Claim 13
Independent Claim
205 words
The method of continuously electrophoretically separating and collecting one or more components of a mixture having components with differing electrophoretic mobility rates in an apparatus having a plurality of separating cells of an electrophoretic supporting medium arranged in a closed cylindrical array, each cell having an input and an output, and said apparatus also having an applicator system for supplying said mixture to said cell inputs and one or more collectors for deriving individual separated components from said cell outputs, comprising the steps of a. rotating said cell array to present each cell successively in rotation to an applicator while each cell is presented to said applicator; b. supplying said cell input with a mixture having components to be separated; c. continuously applying an electric field along each of said cells between its input and output to cause electrophoretic separation of said components along said cell; and d. collecting material from each cell output, in a common collector for all cells, at a substantially fixed time interval following the supplying of material to the input of said cell, said time interval corresponding to the mobility rate of one component of said mixture in said medium, whereby the same component is collected successively from each cell.
Claim 14
Independent Claim
205 words
The method of continuously electrophoretically separating and collecting one or more components of a mixture having components with differing electrophoretic mobility rates in an apparatus having a plurality of separating cells of an electrophoretic supporting medium arranged in a closed cylindrical array, each cell having an input and an output, and said apparatus also having an applicator system for supplying said mixture to said cell inputs and one or more collectors for deriving individual separated components from said cell outputs, comprising the steps of a. rotating said cell array to present each cell successively in rotation to an applicator while each cell is presented to said applicator; b. supplying said cell input with a mixture having components to be separated; c. continuously applying an electric field along each of said cells between its input and output to cause electrophoretic separation of said components along said cell; and d. collecting material from each cell output, in a common collector for all cells, at a substantially fixed time interval following the supplying of material to the input of said cell, said time interval corresponding to the mobility rate of one component of said mixture in said medium, whereby the same component is collected successively from each cell.
Claim 15
Dependent Claim
52 words
The method of claim 11 further comprising additionally collecting material from each cell output at a different substantially fixed time interval following the supplying of material to the input of said cell, said different time interval corresponding to the different mobility rate in said medium of a second component of said mixture.
Claim 16
Dependent Claim
40 words
The method of claim 12 where said first collecting is accomplished at a fixed position along the path of rotation of said cell array, and said second collecting is accomplished at a different fixed position along said cell array path.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-24 | 1978-09-23 | 1979-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-24 | 1982-09-23 | 1983-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-24 | 1986-09-23 | 1987-03-25 | $7700.00 | Window Closed / Lapsed |