US3873426A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Insoluble enzymes
Assignee: Bayer Corp
•
Filing: 1973-07-02
•
Granted: 1975-03-25
•
Expires: 1993-07-02
Abstract
Abstract Water-insoluble enzymes wherein the enzyme is stably retained on an alumina carrier are prepared by adsorbing the enzyme and a dye on an activated alumina carrier at a pH below about 7.
Inventors
- 👤 Ephraim Katchalski
- 👤 Yehuda Levin
- 👤 Beka Solomon
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 22:26
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
51 words
A PROCESS FOR INSOLUBILIZING ENZYMES WHICH COMPRISES ADSORBING AN ENZYME SELECTED FROM THE CLASS CONSISTING OF AMYLOGLUCOSIDASE, LACTASE, BACTERIAL AMYLASE AND GUNGAL AMYLASE AND A HALOTRIAZINE DYE ON AN ACTIVATED ALUMINA CARRIER AT A PH BELOW ABOUT 7 TO PRODUCE AN INSOLUBILIZED ENZYME WHICH RETAINS SIGNIFICANT AMOUNT OF DESIRED ENZYME ACTIVITY.
Claim 2
Dependent Claim
51 words
A PROCESS FOR INSOLUBILIZING ENZYMES WHICH COMPRISES ADSORBING AN ENZYME SELECTED FROM THE CLASS CONSISTING OF AMYLOGLUCOSIDASE, LACTASE, BACTERIAL AMYLASE AND GUNGAL AMYLASE AND A HALOTRIAZINE DYE ON AN ACTIVATED ALUMINA CARRIER AT A PH BELOW ABOUT 7 TO PRODUCE AN INSOLUBILIZED ENZYME WHICH RETAINS SIGNIFICANT AMOUNT OF DESIRED ENZYME ACTIVITY.
Claim 3
Dependent Claim
22 words
A process according to claim 1 wherein the dye is adsorbed on the activated alumina followed by the adsorption of the enzyme.
Claim 4
Dependent Claim
22 words
A process according to claim 1 wherein the enzyme is adsorbed on the activated alumina followed by the adsorption of the dye.
Claim 5
Dependent Claim
19 words
A process according to claim 1 wherein the alumina has a mesh size between about 14 and about 48.
Claim 6
Dependent Claim
15 words
A process according to claim 1 wherein the insolubilized enzyme is further treated with glutaraldehyde.
Claim 7
Dependent Claim
14 words
A process according to claim 1 wherein the dye is a monoazo dichlorotriazine dye.
Claim 8
Dependent Claim
12 words
An insoluble enzyme produced in accordance with the process of claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-24 | 1978-09-23 | 1979-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-24 | 1982-09-23 | 1983-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-24 | 1986-09-23 | 1987-03-25 | $7700.00 | Window Closed / Lapsed |