US3873268A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Multiple solution testing device
Assignee: Warner Lambert Co LLC
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Filing: 1972-08-09
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Granted: 1975-03-25
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Expires: 1992-08-09
Abstract
Abstract Drops of different solutions suspended from the perforations of an upper plate are moved down into contact with a suitable reactive layer or substrate disposed on a lower plate, or the drops may bulge above a welled lower plate which is contacted with an upper reactive layer. The reactions between each of the solutions and reactive layer simultaneously commence when the suspended or upwardly bulging drops contact the reactive layer. A sheet of unexposed but developed photographic film incorporating at least one gelatin layer provides a common reactive layer for comparing a number of trypsin-catalyzed reactions. The film is conveniently handled and inspected when attached to an indexed glass plate. The photographic film is immersed in a low pH bath to simultaneously terminate and preserve a record of all of the inherently concurrent reactions. A simple press with a removable cavitied plate and reactive layer advantageously performs the contact.
Inventors
- 👤 Jr James E Mckie
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 22:14
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5025; B01L3/02; B01L3/0289; G; G01; G01N; G01N31/00; B01L2200/00; B01L2200/06; B01L2200/0642; B01L2400/00; B01L2400/02; B01L2400/022
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
146 words
A METHOD OF SIMULTANEOUSLY TESTING THE REACTING THE REACTIONS NUMBER OF SOLUTIONS WITH A REACTIVE LAYER WHICH COMPRISES THE STEPS OF PLACING SUBSTANTIALLY EQUAL QUANTITIES OF EACH OF SAID SOLUTIONS IN CAVITIES IN A HOLDER WHEREBY SUBSTANTIALLY EQUAL QUANTITIES OF SAID SOLUTIONS ARE CAUSED TO PROJECT SUBSTANTIALLY EQUAL DISTANCES FROM SAID HOLDER, DISPOSING SAID REACTIVE LAYER UPON A PLATE, ALIGNING SAID HOLDER AND SAID PLATE PARALLEL TO EACH OTHER WHEREBY EACH OF THE PROJECTING QUANTITIES IS EQUIDISTANCE FROM SAID REACTIVE LAYER, CAUSING RELATIVE MOVEMENT BETWEEN SAID HOLDER AND SAID PLATE WHILE MAINTAINING SAID PARALLEL RELATIONSHIP FOR DISPOSING THEM CLOSELY ADJACENT EACH OTHER WHEREBY SAID PROJECTING QUANTITIES SIMULTANEOUSLY CONTACT SAID REACTIVE LAYER TO SIMULATNEOUSLY COMMENCE REACTIONS BETWEEN SAID SOLUTIONS AND SAID REACTIVE LAYER, AND SEPARATING SAID HOLDER FROM SAID PLATE WHEREBY SUBSTANTIALLY EQUAL PORTIONS OF SAID PROJECTING QUANTITIES TRANSFER TO SAID REACTIVE LAYER FOR COMPLETING SAID REACTIONS.
Claim 2
Independent Claim
146 words
A METHOD OF SIMULTANEOUSLY TESTING THE REACTING THE REACTIONS NUMBER OF SOLUTIONS WITH A REACTIVE LAYER WHICH COMPRISES THE STEPS OF PLACING SUBSTANTIALLY EQUAL QUANTITIES OF EACH OF SAID SOLUTIONS IN CAVITIES IN A HOLDER WHEREBY SUBSTANTIALLY EQUAL QUANTITIES OF SAID SOLUTIONS ARE CAUSED TO PROJECT SUBSTANTIALLY EQUAL DISTANCES FROM SAID HOLDER, DISPOSING SAID REACTIVE LAYER UPON A PLATE, ALIGNING SAID HOLDER AND SAID PLATE PARALLEL TO EACH OTHER WHEREBY EACH OF THE PROJECTING QUANTITIES IS EQUIDISTANCE FROM SAID REACTIVE LAYER, CAUSING RELATIVE MOVEMENT BETWEEN SAID HOLDER AND SAID PLATE WHILE MAINTAINING SAID PARALLEL RELATIONSHIP FOR DISPOSING THEM CLOSELY ADJACENT EACH OTHER WHEREBY SAID PROJECTING QUANTITIES SIMULTANEOUSLY CONTACT SAID REACTIVE LAYER TO SIMULATNEOUSLY COMMENCE REACTIONS BETWEEN SAID SOLUTIONS AND SAID REACTIVE LAYER, AND SEPARATING SAID HOLDER FROM SAID PLATE WHEREBY SUBSTANTIALLY EQUAL PORTIONS OF SAID PROJECTING QUANTITIES TRANSFER TO SAID REACTIVE LAYER FOR COMPLETING SAID REACTIONS.
Claim 3
Dependent Claim
32 words
A method as set forth in claim 1 wherein an inhibitor is applied to said transferred quantities to simultaneously terminate said reactions whereby said reactions are made substantially concurrent with each other.
Claim 4
Dependent Claim
23 words
A method as set forth in claim 1 wherein said quantities include standard solutions to which the other reactions are compared and evaluated.
Claim 5
Dependent Claim
20 words
A method as set forth in claim 1 wherein a record of said reactions is preserved upon said reactive substrate.
Claim 6
Dependent Claim
29 words
A method as set forth in claim 1 wherein said portions of said quantities are caused to project below said holder by suspension of said portions below said holder.
Claim 7
Dependent Claim
30 words
A method as set forth in claim 1 wherein said portions of said quantities are caused to project from said holder by causing a meniscus to project above said holder.
Claim 8
Independent Claim
177 words
An apparatus for simultaneously testing the reaction of a number of solutions with a reactive layer comprising a solution-holding plate having a number of equal cavities which are constructed and arranged for independently holding and supporting substantially equal quantities of said solutions projecting substantially equal distances from said solution-holding plate, a reaction plate having portions disposed parallel to and equidistantly from corresponding portions of said solution-holding plate, said reaction plate having supporting means for holding said reactive layer, parallel-acting movable coupling means connecting said solution-holding and reaction plates whereby the relative distances between said parallel plates may be parallelly varied from substantially separated to closely adjacent positions, said cavities having a shape and size for retaining said quantities of solution with a portion thereof projecting at said substantially equal distances therefrom whereby said projecting portions simultaneously contact said reactive layer on said reaction plate when said plates are moved into said closely adjacent positions and for substantially transferring equal quantities of said solutionS to said reactive layer on said reaction plate upon subsequent separation of said plates.
Claim 9
Independent Claim
177 words
An apparatus for simultaneously testing the reaction of a number of solutions with a reactive layer comprising a solution-holding plate having a number of equal cavities which are constructed and arranged for independently holding and supporting substantially equal quantities of said solutions projecting substantially equal distances from said solution-holding plate, a reaction plate having portions disposed parallel to and equidistantly from corresponding portions of said solution-holding plate, said reaction plate having supporting means for holding said reactive layer, parallel-acting movable coupling means connecting said solution-holding and reaction plates whereby the relative distances between said parallel plates may be parallelly varied from substantially separated to closely adjacent positions, said cavities having a shape and size for retaining said quantities of solution with a portion thereof projecting at said substantially equal distances therefrom whereby said projecting portions simultaneously contact said reactive layer on said reaction plate when said plates are moved into said closely adjacent positions and for substantially transferring equal quantities of said solutionS to said reactive layer on said reaction plate upon subsequent separation of said plates.
Claim 10
Dependent Claim
41 words
An apparatus as set forth in claim 7 wherein said cavities comprise holes through said plate, said projecting portions of said quantities being suspended from said holes below said plate, and said reaction plate being disposed below said solution holding plate.
Claim 11
Dependent Claim
32 words
An apparatus as set forth in claim 7 wherein said movable coupling means includes a holding element whereby said solution holding plate is maintained in a separated position from said reaction plate.
Claim 12
Dependent Claim
36 words
An apparatus as set forth in claim 7 wherein said movable coupling means includes adjustable stops for varying the distance between said projecting quantities and said reaction plate in said closely adjacent position of said plates.
Claim 13
Dependent Claim
14 words
An apparatus as set forth in claim 7 wherein said cavities are substantially cylindrical.
Claim 14
Dependent Claim
17 words
An apparatus as set forth in claim 11 wherein said cavities have a width of several millimeters.
Claim 15
Dependent Claim
19 words
An apparatus as set forth in claim 7 wherein said movable coupling means comprises rod and slide bearing means.
Claim 16
Dependent Claim
32 words
An apparatus as set forth in claim 13 wherein said solution-holding plate comprises a frame plate and a perforated insert plate, said perforated insert plate being removably mounted in said frame plate.
Claim 17
Dependent Claim
44 words
An apparatus as set forth in claim 7 wherein a removable sheet is disposed on said reaction plate for contact with said drops, and a framed holder being mounted upon said reaction plate for receiving and holding said sheet flat upon said reaction plate.
Claim 18
Dependent Claim
31 words
An apparatus as set forth in claim 15 wherein said sheet comprises photographic film, and a film container facing mounted upon said reaction plate for supplying said film to said holder.
Claim 19
Dependent Claim
43 words
An apparatus as set forth in claim 7 wherein said cavities comprise wells in said plate, said projecting portions of said quantities being menisci of said drops projecting above said drop-holding plate, and said reaction plate being disposed above said solution holding plate.
Claim 20
Dependent Claim
23 words
An apparatus as set forth in claim 17 wherein said solution holding plate comprises a shallow tray incorporating a number of said wells.
Claim 21
Dependent Claim
27 words
An apparatus as set forth in claim 18 wherein said tray and said movable coupling means include tongue and groove means for removable engagement with each other.
Claim 22
Dependent Claim
72 words
An apparatus as set forth in claim 17 wherein said movable coupling means comprises a press having a base and a movable plunger disposed above said base, said base having receiving means for insertion of said solution holding plate, said plunger having removable attaching means for engagement by said reaction plate, and stop means reacting between said plunger and said base for terminating the movement of said plunger toward said reaction plate.
Claim 23
Dependent Claim
42 words
An apparatus as set forth in claim 20 wherein said layer has a platen-supporting plate attached to said plunger, a platen removably engaged with said platen-supporting plate, said platen including a frame, and detachable means removably connecting said platen with said frame.
Claim 24
Dependent Claim
24 words
An apparatus as set forth in claim 21 wherein said detachable means comprises slots in said frame and engaging edges of said platen-supporting plate.
Claim 25
Dependent Claim
38 words
An apparatus as set forth in claim 21 wherein said platen and said solution plate have portions projecting a short distance outwardly from said base to facilitate insertion and removal of said platen and said solution holding plate.
Claim 26
Dependent Claim
23 words
An apparatus as set forth in claim 21 in which a transparent coordinate plate is attached to the lower surface of said frame.
Claim 27
Dependent Claim
16 words
An apparatus as set forth in claim 24 wherein said transparent plate comprises a glass plate.
Claim 28
Dependent Claim
40 words
An apparatus as set forth in claim 24 wherein a slot is disposed on said reaction plate between said film container and said film holder to facilitate the severing of portions of said film in said holder from said container.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-24 | 1978-09-23 | 1979-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-24 | 1982-09-23 | 1983-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-24 | 1986-09-23 | 1987-03-25 | $7700.00 | Window Closed / Lapsed |