US3873216A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Calibration of photoelectric scanner for analytical ultracentrifuge
Assignee: Beckman Coulter Inc
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Filing: 1973-03-19
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Granted: 1975-03-25
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Expires: 1993-03-19
Abstract
Abstract A photoelectric scanner associated with an ultracentrifuge can be calibrated by rotating in the ultracentrifuge a sample cell having, in place of a centerpiece containing sample solution, a disc having two slits, one slit having substantially uniform width, the other slit having stepwise graduated width, and comparing the output signal of the scanner with the known differences in width between the two slits of the disc.
Inventors
- 👤 Lee Gropper
- 👤 Walter W Thurber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 13:54
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/27; G01N21/274; G01N21/276; G01N21/01; G01N21/03; G01N21/07; G01N21/41; G01N21/45
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
200 words
A method of absolute calibration of a photoelectric scanner associated with an analytical ultracentrifuge, the ultracentrifuge having a rotor into which a sample cell having a centerpiece containing sample solution is normally inserted, and the scanner having a slit for receiving ultraviolet light, the slit being stationary with respect to the direction of rotation of the rotor and movable in the radial direction of the rotor, which method comprises inserting into the rotor a sample cell having, in place of the centerpiece, a disc having two slits, one slit having substantially uniform width, the other slit having stepwise graduated width, the length of each step being greater than the width of the slit of the scanner, rotating the sample cell in the ultracentrifuge, passing ultraviolet light through the rotating sample cell, moving the slit of the scanner radially with respect to the rotor, receiving and processing in the scanner light passing through the cell to produce electrical signals representative of the differences in the intensity of the light transmitted by the slits of the disc, recording the electrical signals, and comparing the value of the electrical signals with the corresponding differences in width between the two slits of the disc.
Claim 2
Independent Claim
200 words
A method of absolute calibration of a photoelectric scanner associated with an analytical ultracentrifuge, the ultracentrifuge having a rotor into which a sample cell having a centerpiece containing sample solution is normally inserted, and the scanner having a slit for receiving ultraviolet light, the slit being stationary with respect to the direction of rotation of the rotor and movable in the radial direction of the rotor, which method comprises inserting into the rotor a sample cell having, in place of the centerpiece, a disc having two slits, one slit having substantially uniform width, the other slit having stepwise graduated width, the length of each step being greater than the width of the slit of the scanner, rotating the sample cell in the ultracentrifuge, passing ultraviolet light through the rotating sample cell, moving the slit of the scanner radially with respect to the rotor, receiving and processing in the scanner light passing through the cell to produce electrical signals representative of the differences in the intensity of the light transmitted by the slits of the disc, recording the electrical signals, and comparing the value of the electrical signals with the corresponding differences in width between the two slits of the disc.
Claim 3
Independent Claim
175 words
A device for absolute calibration of a photoelectric scanner associated with an analytical ultracentrifuge, the ultracentrifuge having a rotor into which a sample cell having a centerpiece containing sample solution is normally inserted, and the scanner having a slit for receiving ultraviolet light, the slit being stationary with respect to the direction of rotation of the rotor and movable in the radial direction of the rotor, which device comprises a disc having two slits, one slit having a substantially uniform width, the other slit having substantially stepwise graduated width, the disc being capable of being incorporated into the sample cell in place of the centerpiece normally present therein, whereby, when the disc is rotated past the slit of the scanner, the peak transmissions of light through the slit of the scanner are proportional to the widths of the respective slits of the disc, thereby permitting calibration of the scanner by comparison of the output of the scanner with the corresponding known differences in width between the respective portions of the two slits of the disc.
Claim 4
Independent Claim
175 words
A device for absolute calibration of a photoelectric scanner associated with an analytical ultracentrifuge, the ultracentrifuge having a rotor into which a sample cell having a centerpiece containing sample solution is normally inserted, and the scanner having a slit for receiving ultraviolet light, the slit being stationary with respect to the direction of rotation of the rotor and movable in the radial direction of the rotor, which device comprises a disc having two slits, one slit having a substantially uniform width, the other slit having substantially stepwise graduated width, the disc being capable of being incorporated into the sample cell in place of the centerpiece normally present therein, whereby, when the disc is rotated past the slit of the scanner, the peak transmissions of light through the slit of the scanner are proportional to the widths of the respective slits of the disc, thereby permitting calibration of the scanner by comparison of the output of the scanner with the corresponding known differences in width between the respective portions of the two slits of the disc.
Claim 5
Dependent Claim
26 words
The device of claim 2 wherein the length of each step of the graduated slit is greater than the width of the slit of the scanner.
Claim 6
Dependent Claim
25 words
The device of claim 3 wherein the distance between the slits of the disc is greater than the length of the slit of the scanner.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-24 | 1978-09-23 | 1979-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-24 | 1982-09-23 | 1983-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-24 | 1986-09-23 | 1987-03-25 | $7700.00 | Window Closed / Lapsed |