US3871963A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Microbial protease and preparation thereof
Assignee: Ajinomoto Co Inc
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Filing: 1973-03-13
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Granted: 1975-03-18
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Expires: 1993-03-13
Abstract
Abstract A strain of Bacillus produces a new alkaline protease. The protease is superior to known alkaline proteases in thermostability and resistance to chelating agents, and works at higher pH.
Inventors
- 👤 Sadanobu Tobe
- 👤 Yoshiteru Hirose
- 👤 Yoshihiro Nakamura
- 👤 Koji Mitsugi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 15:55
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
81 words
A METHOD OF PRODUCING A PROTEASEE HAIVNG OPTIMUM PH BETWEEN 11 AND 12 AND BEING STABLE AT 5.5 TO 9.0 WHEN HEATED 30 MINUTES TO 50*C IN AN AQUEOUS MEDIUM WHICH COMPRISES: A. CULTURING THE GRAM-POSITIVE, ASPOROGENSOUS STRAIN BACILLUS SP. FERM P-1396 IN AN AQUEOUS NUTRIENT MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON AND NITROGEN INORGANIC SALTS AND MINOR ORGANIC NUTRIENTS UNTIL A SIGNIFICANT AMOUNT OF SAID PROTEASE IS ACCUMULATED IN SAID MEDIUM; AND B. RECOVERING THE ACCUMULATED PROTEASE FROM SAID MEDIUM.
Claim 2
Independent Claim
81 words
A METHOD OF PRODUCING A PROTEASEE HAIVNG OPTIMUM PH BETWEEN 11 AND 12 AND BEING STABLE AT 5.5 TO 9.0 WHEN HEATED 30 MINUTES TO 50*C IN AN AQUEOUS MEDIUM WHICH COMPRISES: A. CULTURING THE GRAM-POSITIVE, ASPOROGENSOUS STRAIN BACILLUS SP. FERM P-1396 IN AN AQUEOUS NUTRIENT MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON AND NITROGEN INORGANIC SALTS AND MINOR ORGANIC NUTRIENTS UNTIL A SIGNIFICANT AMOUNT OF SAID PROTEASE IS ACCUMULATED IN SAID MEDIUM; AND B. RECOVERING THE ACCUMULATED PROTEASE FROM SAID MEDIUM.
Claim 3
Dependent Claim
57 words
A microbial protease prepared by the method set forth in claim 1, said protease having an optimum pH between 11 and 12, a molecular weight between 15,000 and 20,000, as measured by the sedimentation equilibrium method, an isoelectric point above pH 10, said protease being stable at pH 5.5 to 9.0 when heated 30 minutes at 50*C.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-17 | 1978-09-16 | 1979-03-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-17 | 1982-09-16 | 1983-03-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-17 | 1986-09-16 | 1987-03-18 | $7700.00 | Window Closed / Lapsed |