US3870602A — Gas permeable sterile culture bottle

Claim 1

Independent Claim 172 words

A STERILE, DISPOSABLE CULTURE BOTTLE FOR GROWTH OF MICROORGANISMS IN A CONTROLLED ENVIRONMENT OF A GAS AND WATER VAPOR COMPRISING: AN ELONGATED FLASK-LIKE MEMBER INCLUDING A BOTTOM WALL AND A SPACED TOP WALL; SAID, WALLS IN TEGEGRAL WITH SAID BOTTOM WALL AND DEFINING THEREWITH AND WITH SAID TOP WALL A FLASK OF UNITARY STRUCTURE; ONE OF SAID SIDE WALLS INCLUDING A NECK INTEGRAL WITH SAID SIDE ARRANGED AT AN UPWARDLY INCLINED ANGLE WITH RESPECT TO SAID BOTTOM WALL, ALL OF SAID WALLS AND SAID NECK BEING INSEPARABLY SECURED TO EACH OTHER, SAID NECK HAVING A FREE END TO RECEIVE A CAP SEALABLY ENGAGING SAID NECK TO PREVENT PASSAGE OF MICROORGANSIMS THERETHROUGH; SAID WALLS OF SAID FLASK BEING OF AN IMPACT RESISTANT PLASTIC MATERIAL PERMEABLE TO SAID GAS AND WATER VAPOR, BUT IMPERMEABLE TO MICROORGANISMS; SAID TOP WALL BEING ESSENTIALLY FLAT, TRANSPARENT AND OPTICALLY DISTORTION FREE SO AS TO PERMIT MICROSCOPIC EXAMINATION OF THE CULTURE WITHIN SAID FLASK, AND MEANS ON SAID BOTTLE FOR MAINTAINING SAID BOTTOM WALL SPACED FROM A SUPPORTING SURFACE.

Claim 2

Dependent Claim 172 words

A STERILE, DISPOSABLE CULTURE BOTTLE FOR GROWTH OF MICROORGANISMS IN A CONTROLLED ENVIRONMENT OF A GAS AND WATER VAPOR COMPRISING: AN ELONGATED FLASK-LIKE MEMBER INCLUDING A BOTTOM WALL AND A SPACED TOP WALL; SAID, WALLS IN TEGEGRAL WITH SAID BOTTOM WALL AND DEFINING THEREWITH AND WITH SAID TOP WALL A FLASK OF UNITARY STRUCTURE; ONE OF SAID SIDE WALLS INCLUDING A NECK INTEGRAL WITH SAID SIDE ARRANGED AT AN UPWARDLY INCLINED ANGLE WITH RESPECT TO SAID BOTTOM WALL, ALL OF SAID WALLS AND SAID NECK BEING INSEPARABLY SECURED TO EACH OTHER, SAID NECK HAVING A FREE END TO RECEIVE A CAP SEALABLY ENGAGING SAID NECK TO PREVENT PASSAGE OF MICROORGANSIMS THERETHROUGH; SAID WALLS OF SAID FLASK BEING OF AN IMPACT RESISTANT PLASTIC MATERIAL PERMEABLE TO SAID GAS AND WATER VAPOR, BUT IMPERMEABLE TO MICROORGANISMS; SAID TOP WALL BEING ESSENTIALLY FLAT, TRANSPARENT AND OPTICALLY DISTORTION FREE SO AS TO PERMIT MICROSCOPIC EXAMINATION OF THE CULTURE WITHIN SAID FLASK, AND MEANS ON SAID BOTTLE FOR MAINTAINING SAID BOTTOM WALL SPACED FROM A SUPPORTING SURFACE.

Claim 3

Dependent Claim 171 words

A sterile, disposable culture bottle for growth of microorganisms in a controlled environment of a gas and water vapor comprising: an elongated flask-like member including a bottom wall and a spaced top wall; side walls integral with said bottom wall and defining therewith and with said top wall a flask of unitary structure; one of said side walls including a neck integral with said side arranged at an upwardly inclined angle with respect to said bottom wall, all of said walls and said neck being inseparably secured to each other, said neck having a free end to receive a cap sealably engaging said neck to prevent passage of microorganisms therethrough; said walls of said flask being of an impact resistant plastic material permeable to said gas and water vapor, but impermeable to microorganisms; said top wall being essentially flat, transparent and optically distortion free so as to permit microscopic examination of the culture within said flask, and means on said bottle for maintaining said bottom wall spaced from a supporting surface.

Claim 4

Dependent Claim 171 words

A sterile, disposable culture bottle for growth of microorganisms in a controlled environment of a gas and water vapor comprising: an elongated flask-like member including a bottom wall and a spaced top wall; side walls integral with said bottom wall and defining therewith and with said top wall a flask of unitary structure; one of said side walls including a neck integral with said side arranged at an upwardly inclined angle with respect to said bottom wall, all of said walls and said neck being inseparably secured to each other, said neck having a free end to receive a cap sealably engaging said neck to prevent passage of microorganisms therethrough; said walls of said flask being of an impact resistant plastic material permeable to said gas and water vapor, but impermeable to microorganisms; said top wall being essentially flat, transparent and optically distortion free so as to permit microscopic examination of the culture within said flask, and means on said bottle for maintaining said bottom wall spaced from a supporting surface.

Claim 5

Dependent Claim 19 words

A culture bottle as defined in claim 1, wherein said bottle contains a solid media for culturing of microorganisms.

Claim 6

Dependent Claim 13 words

A culture bottle as defined in claim 1, wherein said plastic is polystyrene.

Claim 7

Dependent Claim 76 words

A culture bottle as defined in claim 3, wherein said polystyrene has the following permeability at no pressure differential: carbon dioxide 2.833 ml/24 hrs. at 23*C and 4.166 ml/24 hrs. at 37*C, oxygen 1.166 ml/24 hrs. at 23*C and 1.166 ml/24 hrs. at 37*C, water vapor 0.0075 g/24 hrs. at 23*C and 0.0090 g/24 hrs. at 37*C, said water vapor permeability being measured at 100% R.H. within the bottle and 50% R.H. outside of said bottle.

Claim 8

Dependent Claim 29 words

A culture bottle as defined in claim 1, wherein said plastic has an Izod impact of 2.4 cm. Kg. f/cm. of notch at 23*C as determined by ASTMD 256-56.

Claim 9

Dependent Claim 26 words

A sterile culture bottle as defined in claim 1; wherein said neck is arranged at an angle of about 10* with respect to said bottom wall.

Claim 10

Dependent Claim 46 words

A sterile culture bottle as defined in claim 1, wherein said bottom wall is essentially flat, and said top wall being in essentially spaced parallel relative to said bottom wall thereby permitting microscopic examination through said top wall of the culture being grown with said flask.

Claim 11

Dependent Claim 30 words

A sterile culture bottle as set forth in claim 5, wherein said top wall is essentially flat and of the same plastic material as the remainder of said flask-like member.

Claim 12

Dependent Claim 37 words

A sterile culture bottle as set forth in claim 1, wherein said side walls include transition walls adjacent said one wall which includes said neck thereby eliminating right-angle walls adjacent to said wall which includes said neck.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1978-03-10	1978-09-09	1979-03-11	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1982-03-10	1982-09-09	1983-03-11	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	1986-03-10	1986-09-09	1987-03-11	$7700.00	Window Closed / Lapsed

Gas permeable sterile culture bottle

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