US3869547A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Calf diarrhea virus vaccine and processes
Assignee: University of Nebraska–Lincoln
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Filing: 1973-11-07
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Granted: 1975-03-04
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Expires: 1993-11-07
Abstract
Abstract Calf diarrhea virus vaccines are prepared by passaging calf diarrhea virus obtained from feces of infected calves in tissue culture, particularly bovine or swine tissue culture, from one to about 250 times. Inactivated vaccines are also prepared.
Inventors
- 👤 Charles A Mebus
- 👤 Marvin J Twiehaus
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 00:06
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/15; A61K2039/51; A61K2039/525; A61K2039/5254; A61K2039/555; A61K2039/55511; A61K2039/55566; C; C12; C12N; C12N2720/00; C12N2720/00011; C12N2720/12011; C12N2720/12034; Y; Y10; Y10S; Y10S424/00; Y10S424/813
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
94 words
AN ATTENUATED REOVIRUS-LIKE CALF- DIARRHEA VIRUS WHICH HAS BE PRISING (1) A REOVIRUS-LIKE CALF-DIARRHEA VIRUS WHICH HAS BEEN ATTENUATED BY PASSAGING A VIRULENT REOVIRUS-LIKE CALF-DIARRHEA VIRUS IDENTIFIED BY IMMUNOFLUORESCENCE, IN TISSUE CULTURE WHICH SUPPORTS GROWTH OF THE VIRUS, CONTINUING UNTIL A NOTICEABLE CYTOPATHOLOGICAL EFFECTS IS OBSERVED, SAID CYTOPATHOLOGICAL EFFECT BEING APPARENT FROM FLAGGING PRODUCED OR INFECTION FOLLOWED BY IMMUNOFLUORESCENCE, FROM ABOUT 37 TO 250 TIMES UNTIL THE ATTENUATED VIRUS, WHILE INDUCING IMMUNITY IN NEONATAL CALVES, DOES NOT PRODUCE DISEASE, AND (2) A CARRIER, THE TITE OF SAID ATTENUATED VIRUS BEING AT LEAST 10**2 TCID/ML.
Claim 2
Dependent Claim
94 words
AN ATTENUATED REOVIRUS-LIKE CALF- DIARRHEA VIRUS WHICH HAS BE PRISING (1) A REOVIRUS-LIKE CALF-DIARRHEA VIRUS WHICH HAS BEEN ATTENUATED BY PASSAGING A VIRULENT REOVIRUS-LIKE CALF-DIARRHEA VIRUS IDENTIFIED BY IMMUNOFLUORESCENCE, IN TISSUE CULTURE WHICH SUPPORTS GROWTH OF THE VIRUS, CONTINUING UNTIL A NOTICEABLE CYTOPATHOLOGICAL EFFECTS IS OBSERVED, SAID CYTOPATHOLOGICAL EFFECT BEING APPARENT FROM FLAGGING PRODUCED OR INFECTION FOLLOWED BY IMMUNOFLUORESCENCE, FROM ABOUT 37 TO 250 TIMES UNTIL THE ATTENUATED VIRUS, WHILE INDUCING IMMUNITY IN NEONATAL CALVES, DOES NOT PRODUCE DISEASE, AND (2) A CARRIER, THE TITE OF SAID ATTENUATED VIRUS BEING AT LEAST 10**2 TCID/ML.
Claim 3
Dependent Claim
20 words
A vaccine according to claim 1, in which the virulent virus has been passaged in bovine kidney cells at 28*-38*.
Claim 4
Dependent Claim
16 words
A vaccine according to claim 2 in which the virulent virus has been passaged 130-250 times.
Claim 5
Dependent Claim
17 words
A vaccine according to claim 3, in which the virulent virus has been passaged approximately 194 times.
Claim 6
Dependent Claim
76 words
An attenuated reovirus-like calf diarrhea vaccine comprising (1) an attenuated reovirus-like calf diarrhea virus which has been prepared by growing in a suitable cell culture a further amount of a reovirus-like calf diarrhea virus, attenuated as in claim 1 said growth being for a period of time and number of passages sufficient to permit growth of a larger amount of said virus and (2) a carrier, the titer of said virus being at least 102 TCID/ml.
Claim 7
Dependent Claim
77 words
An attenuated reovirus-like calf diarrhea vaccine comprising (1) an attenuated reovirus-like calf diarrhea virus which has been prepared by growing in a bovine kidney cell culture a further amount of a reovirus-like calf diarrhea virus, attenuated as in claim 3, said growth being for a period of time and number of passages sufficient to permit growth of a larger amount of said virus and (2) a carrier, the titer of said virus being at least 102 TCID/ml.
Claim 8
Dependent Claim
77 words
An attenuated reovirus-like calf diarrhea vaccine comprising (1) an attenuated reovirus-like calf diarrhea virus which has been prepared by growing in a bovine kidney cell culture a further amount of a reovirus-like calf Diarrhea virus, attenuated as in claim 4, said growth being for a period of time and number of passages sufficient to permit growth of a larger amount of said virus and (2) a carrier, the titer of said virus being at least 102 TCID/ml.
Claim 9
Dependent Claim
32 words
A method of immunizing newborn calves against reovirus-like calf diarrhea disease comprising orally administering to a newborn calf shortly after birth an attenuated reovirus-like calf diarrhea vaccine as claimed in claim 5.
Claim 10
Dependent Claim
32 words
A method of immunizing newborn calves against reovirus-like calf diarrhea disease comprising orally administering to a newborn calf shortly after birth an attenuated reovirus-like calf diarrhea vaccine as claimed in claim 6.
Claim 11
Dependent Claim
32 words
A method of immunizing newborn calves against reovirus-like calf diarrhea disease comprising orally administering to a newborn calf shortly after birth an attenuated reovirus-like calf diarrhea vaccine as claimed in claim 7.
References Cited (Backward Citations)
US3293129A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-03 | 1978-09-02 | 1979-03-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-03 | 1982-09-02 | 1983-03-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-03 | 1986-09-02 | 1987-03-04 | $7700.00 | Window Closed / Lapsed |