US3869067A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus for gradient elution
Assignee: EIDP Inc
•
Filing: 1974-01-14
•
Granted: 1975-03-04
•
Expires: 1994-01-14
Abstract
Abstract The use of separate supply means for each of two liquids and a proportioning means comprising a mixing region, valving means connected to the mixing chamber to control the flow of each liquid to the mixing region, and a means for periodically controlling the operation of the valving means to control the amount of each liquid supplied to the mixing region during each period of valve operation, produces a supply of eluent having a precisely controlled time varying concentration of each liquid.
Inventors
- 👤 Howard L Ashmead
- 👤 Jr Sydnor H Byrne
- 👤 Iii John P Wolf
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 05:08
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/26; G01N30/28; G01N30/34; G05; G05D; G05D11/00; G05D11/02; G05D11/13; G05D11/131; G05D11/132; Y; Y10; Y10T; Y10T137/00; Y10T137/8593; Y10T137/86389; Y10T137/86445
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
261 words
An elution system comprising: a. a source of first liquid; b. a source of second liquid; c. first liquid supply means connected to said source of first liquid; d. second liquid supply means connected to said source of second liquid; e. proportioning means comprising a mixing region, valving means connected to said mixing region, and programming means connected to said valving means for continuously changing the concentration of the first and second liquids in the mixing region, said valving means having at least two operative portions, a first operative portion connected between said first liquid supply means and said mixing region in a manner such as to supply said mixing region with said first liquid when said first operative portion is activated and a second operative portion connected between at least a portion of said second liquid supply means and said mixing region in a manner such as to supply said mixing region with said second liquid when said second operative portion is activated, said programming means being adapted to separately and periodically control the time during which each operative portion of said valving means is activated and thereby change the concentration of the liquids in the mixing region, said mixing region having a volume greater than the maximum volume of liquid entering said mixing region during the period when either operative portion of said valving means is activated, and comprising means to allow a portion of any liquid contained in said mixing region to be removed from said mixing region while the liquids are being added to the mixing region.
Claim 2
Dependent Claim
261 words
An elution system comprising: a. a source of first liquid; b. a source of second liquid; c. first liquid supply means connected to said source of first liquid; d. second liquid supply means connected to said source of second liquid; e. proportioning means comprising a mixing region, valving means connected to said mixing region, and programming means connected to said valving means for continuously changing the concentration of the first and second liquids in the mixing region, said valving means having at least two operative portions, a first operative portion connected between said first liquid supply means and said mixing region in a manner such as to supply said mixing region with said first liquid when said first operative portion is activated and a second operative portion connected between at least a portion of said second liquid supply means and said mixing region in a manner such as to supply said mixing region with said second liquid when said second operative portion is activated, said programming means being adapted to separately and periodically control the time during which each operative portion of said valving means is activated and thereby change the concentration of the liquids in the mixing region, said mixing region having a volume greater than the maximum volume of liquid entering said mixing region during the period when either operative portion of said valving means is activated, and comprising means to allow a portion of any liquid contained in said mixing region to be removed from said mixing region while the liquids are being added to the mixing region.
Claim 3
Dependent Claim
32 words
The elution system of claim 1 wherein said valving means is a three-way valve, one path therEin being said first operative portion and the second path therein being said second operative portion.
Claim 4
Dependent Claim
32 words
The elution system of claim 1 wherein said valving means comprises two separate on-off valves, the first valve being said first operative portion and the second valve being said second operative portion.
Claim 5
Dependent Claim
241 words
An elution system comprising: a. a source of first liquid; b. a source of second liquid; c. a first pump connected to said source of first liquid; d. a second pump connected to said source of second liquid; and e. proportioning means comprising a mixing region, valving means connected to said mixing region, and programming means connected to said valving means for continuously changing the concentration of the first and second liquids in the mixing region, said valving means having two operative portions, a first operative portion connected to said first pump in a manner such as to supply said mixing region with said first liquid when said first operative portion is activated, and a second operative portion connected to said second pump in a manner such as to supply said mixing region with said second liquid when said second operative portion is activated, said programming means being adapted to separately and periodically control the time during which each operative portion of said valving means is activated and thereby change the concentration of the liquids in the mixing region, said mixing region having a volume greater than the maximum volume of liquid entering said mixing region during the period when either operative portion of said valving means is activated, and comprising means to allow a portion of any liquid contained in said mixing region to be removed from said mixing region while the liquids are being added to the mixing region.
Claim 6
Dependent Claim
241 words
An elution system comprising: a. a source of first liquid; b. a source of second liquid; c. a first pump connected to said source of first liquid; d. a second pump connected to said source of second liquid; and e. proportioning means comprising a mixing region, valving means connected to said mixing region, and programming means connected to said valving means for continuously changing the concentration of the first and second liquids in the mixing region, said valving means having two operative portions, a first operative portion connected to said first pump in a manner such as to supply said mixing region with said first liquid when said first operative portion is activated, and a second operative portion connected to said second pump in a manner such as to supply said mixing region with said second liquid when said second operative portion is activated, said programming means being adapted to separately and periodically control the time during which each operative portion of said valving means is activated and thereby change the concentration of the liquids in the mixing region, said mixing region having a volume greater than the maximum volume of liquid entering said mixing region during the period when either operative portion of said valving means is activated, and comprising means to allow a portion of any liquid contained in said mixing region to be removed from said mixing region while the liquids are being added to the mixing region.
Claim 7
Dependent Claim
32 words
The elution system of claim 4 wherein said valving means is a three-way valve, one path therein being said first operative portion and the second path therein being said second operative portion.
Claim 8
Dependent Claim
32 words
The elution system of claim 4 wherein said valving means comprising two separate on-off valves, the first valve being said first operative portion and the second valve being the second operative portion.
Claim 9
Dependent Claim
13 words
The elution system of claim 4 wherein said pumps are constant pressure pumps.
Claim 10
Dependent Claim
13 words
The elution system of claim 4 wherein said pumps are constant flow pumps.
Claim 11
Dependent Claim
221 words
An elution system comprising: a. a source of first liquid; b. a source of second liquid; c. proportioning means comprising a mixing region, a first on-off valve connected to said mixing region, a second on-off valve connected to said mixing region, and programming means connected to both said first and second on-off valves for continuously changing the concentration of the first and second liquids in the mixing region, said programming means being adapted to separately and periodically control the duration during which each of said on-off valves is open and thereby change the concentration of the liquids in the mixing region, said mixing region having a volume greater than the maximum volume of liquid entering said mixing region during the period when either operative portion of said valving means is activated, and comprising means to continuously allow a portion of any liquid contained in said mixing region to leave said mixing region while the liquids are being added to the mixing region; d. first liquid supply means connected between said source of first liquid and said proportioning means to supply said first on-off valve with said first liquid at constant pressure; and e. second liquid supply means connected between said source of second liquid and said proportioning means to supply said second on-off valve with said second liquid at constant pressure.
Claim 12
Dependent Claim
221 words
An elution system comprising: a. a source of first liquid; b. a source of second liquid; c. proportioning means comprising a mixing region, a first on-off valve connected to said mixing region, a second on-off valve connected to said mixing region, and programming means connected to both said first and second on-off valves for continuously changing the concentration of the first and second liquids in the mixing region, said programming means being adapted to separately and periodically control the duration during which each of said on-off valves is open and thereby change the concentration of the liquids in the mixing region, said mixing region having a volume greater than the maximum volume of liquid entering said mixing region during the period when either operative portion of said valving means is activated, and comprising means to continuously allow a portion of any liquid contained in said mixing region to leave said mixing region while the liquids are being added to the mixing region; d. first liquid supply means connected between said source of first liquid and said proportioning means to supply said first on-off valve with said first liquid at constant pressure; and e. second liquid supply means connected between said source of second liquid and said proportioning means to supply said second on-off valve with said second liquid at constant pressure.
Claim 13
Dependent Claim
26 words
The elution system of claim 9 wherein said first liquid supply means is a first pump and said second liquid supply means is a second pump.
Claim 14
Dependent Claim
17 words
The elution system of claim 9 wherein said first and second on-off valves are air actuated valveS.
Claim 15
Dependent Claim
16 words
The elution system of claim 9 wherein said first and second on-off valves are solenoid valves.
Claim 16
Dependent Claim
60 words
The elution system of claim 9 wherein said first and second on-off valves are normally closed solenoid valves and wherein said programming means is a means to generate an electric signal which will activate either of said on-off solenoid valves and to apply said signal to at least one of said on-off solenoid valves for a predetermined period of time.
Claim 17
Dependent Claim
32 words
The elution system of claim 9 wherein said programming means is a means to generate a time varying signal and to apply said signal alternately to each of said on-off solenoid valves.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-03 | 1978-09-02 | 1979-03-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-03 | 1982-09-02 | 1983-03-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-03 | 1986-09-02 | 1987-03-04 | $7700.00 | Window Closed / Lapsed |