US3864477A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Compositions
Assignee: Laboratoires Albert Rolland SA
•
Filing: 1973-04-12
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Granted: 1975-02-04
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Expires: 1993-04-12
Abstract
Abstract The invention provides pharmaceutical and cosmetic compositions for application to skin showing elastic tissue changes comprising an enzymatic material having elastolytic activity and a vehicle.
Inventors
- 👤 Yvonne Thuillier
- 👤 Marcel Antoine Perrault
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 16:36
CPC Classifications
A; A61; A61Q; A61Q19/00; A61Q19/08; A61K; A61K38/00; A61K38/16; A61K38/43; A61K38/46; A61K38/48; A61K38/482; A61K38/486; A61K8/00; A61K8/18; A61K8/30; A61K8/64; A61K8/66
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
15 words
CONVENTIONAL EXTRACTION OF THE PANCREAS WITH ACETONE AND ETHER TO OBTAIN A DELIPIDATED PANCREAS POWDER;
Claim 2
Dependent Claim
15 words
CONVENTIONAL EXTRACTION OF THE PANCREAS WITH ACETONE AND ETHER TO OBTAIN A DELIPIDATED PANCREAS POWDER;
Claim 3
Independent Claim
121 words
A METHOD FOR TREATING STRIA DISTENSAE, AND PSEUDOXANTHOMA ELASTICUM WHICH COMPRISES APPLYING TOPICALLY TO A HUMAN HOST IN NEED OF SUCH TREATMENT A COSMETIC COMPOSITION IN A THERAPEUTICALLY EFFECTIVE AMOUNT, COMPRISING AN ELASTOYLTICALLY ACTIVE ENZYMATIC MATERIAL PREPARED FROM DELIPIDATED PANCREATIC MATERIAL, SAID ENZYMATIC MATERIAL HAVING AN ISOELECTRIC POINT OF ABOUT 9.5 $ 0.5, A SLIGHT SOLUBILITY IN WATER HAVING A PH BELOW 4.5 PEAKS IN THE ULTRAVOILET SPECTRUM AT 2,200 AND 2,800 A AND HAVING TWO MAIN CONSTITUENTS, THE FIRST CONSTITUENT OF SAID ENZYMATIC MATERIAL ELECTROPHORETICALLY DEMONSTRATES A MOBILITY SLOWER THAN GAMMA GLOBULINS AND DIGESTS ELASTIN AND THE SECOND CONSTITUENT ELECTROPHORETICALLY DEMONSTRATES A MOBILITY COMPARABLE TO B1 AND B2 GLOBULINS, SAID ENZYMATIC MATERIAL BEING PRODUCED IN ACCORDANCE WITH THE FOLLOWING STEPS:
Claim 4
Independent Claim
121 words
A METHOD FOR TREATING STRIA DISTENSAE, AND PSEUDOXANTHOMA ELASTICUM WHICH COMPRISES APPLYING TOPICALLY TO A HUMAN HOST IN NEED OF SUCH TREATMENT A COSMETIC COMPOSITION IN A THERAPEUTICALLY EFFECTIVE AMOUNT, COMPRISING AN ELASTOYLTICALLY ACTIVE ENZYMATIC MATERIAL PREPARED FROM DELIPIDATED PANCREATIC MATERIAL, SAID ENZYMATIC MATERIAL HAVING AN ISOELECTRIC POINT OF ABOUT 9.5 $ 0.5, A SLIGHT SOLUBILITY IN WATER HAVING A PH BELOW 4.5 PEAKS IN THE ULTRAVOILET SPECTRUM AT 2,200 AND 2,800 A AND HAVING TWO MAIN CONSTITUENTS, THE FIRST CONSTITUENT OF SAID ENZYMATIC MATERIAL ELECTROPHORETICALLY DEMONSTRATES A MOBILITY SLOWER THAN GAMMA GLOBULINS AND DIGESTS ELASTIN AND THE SECOND CONSTITUENT ELECTROPHORETICALLY DEMONSTRATES A MOBILITY COMPARABLE TO B1 AND B2 GLOBULINS, SAID ENZYMATIC MATERIAL BEING PRODUCED IN ACCORDANCE WITH THE FOLLOWING STEPS:
Claim 5
Dependent Claim
17 words
EXTRACTION OF SAID POWDER WITH AN M/100 SODIUM ACETATE BUFFER AT A PH BETWEEN 4.8 AND 5:
Claim 6
Dependent Claim
17 words
EXTRACTION OF SAID POWDER WITH AN M/100 SODIUM ACETATE BUFFER AT A PH BETWEEN 4.8 AND 5:
Claim 7
Dependent Claim
71 words
A method according to claim 1 wherein the cosmetic composition comprises 10 parts by weight of said enzymatic material, 250 parts by weight of calcium chloride, 100 parts by weight of polyethylene glycols selected from the group consisting of those polyethylene glycols having mean molecular weights of 400 and 4,000 in equal quantities and 0.02 parts by weight of perfume, said composition having a pH of about 6.8 to about 8.0.
Claim 8
Dependent Claim
17 words
extraction of said powder with an M/100 sodium acetate buffer at a pH between 4.8 and 5;
Claim 9
Dependent Claim
17 words
extraction of said powder with an M/100 sodium acetate buffer at a pH between 4.8 and 5;
Claim 10
Dependent Claim
20 words
precipitation of active material by addition to extract of ammonium sulfate in a concentration of between about 48 to 55%;
Claim 11
Dependent Claim
20 words
precipitation of active material by addition to extract of ammonium sulfate in a concentration of between about 48 to 55%;
Claim 12
Dependent Claim
20 words
PRECIPITATION OF ACTIVE MATERIAL BY ADDITION TO EXTRACT OF AMMONIUM SULFATE IN A CONCENTRATION OF BETWEEN ABOUT 48 TO 55%;
Claim 13
Dependent Claim
20 words
PRECIPITATION OF ACTIVE MATERIAL BY ADDITION TO EXTRACT OF AMMONIUM SULFATE IN A CONCENTRATION OF BETWEEN ABOUT 48 TO 55%;
Claim 14
Dependent Claim
14 words
A method according to claim 1, wherein the disease being treated is stria distensae.
Claim 15
Dependent Claim
9 words
demineralization of said precipitate of active material by dialysis;
Claim 16
Dependent Claim
9 words
demineralization of said precipitate of active material by dialysis;
Claim 17
Dependent Claim
9 words
DEMINERALIZATION OF SAID PRECIPITATE OF ACTIVE MATERIAL BY DIALYSIS;
Claim 18
Dependent Claim
9 words
DEMINERALIZATION OF SAID PRECIPITATE OF ACTIVE MATERIAL BY DIALYSIS;
Claim 19
Dependent Claim
38 words
fractional precipitation with ethanol or methanol comprising the steps of: a. a first precipitation in about 50% ethanol or methanol to remove inactive material, and b. a second precipitation in about 80% ethanol or methanol of active material;
Claim 20
Dependent Claim
38 words
fractional precipitation with ethanol or methanol comprising the steps of: a. a first precipitation in about 50% ethanol or methanol to remove inactive material, and b. a second precipitation in about 80% ethanol or methanol of active material;
Claim 21
Dependent Claim
38 words
FRACTIONAL PRECIPITATION WITH ETHANOL OR METHANOL COMPRISING THE STEPS OF: A. A FIRST PRECIPITATION IN ABOUT 50% ETHANOL OR METHANOL TO REMOVE INACTIVE MATERIAL, AND B. A SECOND PRECIPITATION IN ABOUT 80% ETHANOL OR METHANOL OF ACTIVE MATERIAL;
Claim 22
Dependent Claim
38 words
FRACTIONAL PRECIPITATION WITH ETHANOL OR METHANOL COMPRISING THE STEPS OF: A. A FIRST PRECIPITATION IN ABOUT 50% ETHANOL OR METHANOL TO REMOVE INACTIVE MATERIAL, AND B. A SECOND PRECIPITATION IN ABOUT 80% ETHANOL OR METHANOL OF ACTIVE MATERIAL;
Claim 23
Dependent Claim
18 words
SOLUTION OF ACTIVE MATERIAL IN AN M/100 SODIUM ACETATE BUFFER AT A PH BETWEEN 4.7 AND 5; AND
Claim 24
Dependent Claim
18 words
SOLUTION OF ACTIVE MATERIAL IN AN M/100 SODIUM ACETATE BUFFER AT A PH BETWEEN 4.7 AND 5; AND
Claim 25
Dependent Claim
18 words
solution of active material in an M/100 sodium acetate buffer at a pH between 4.7 and 5; and
Claim 26
Dependent Claim
18 words
solution of active material in an M/100 sodium acetate buffer at a pH between 4.7 and 5; and
Claim 27
Dependent Claim
77 words
LYOPHILIZATION, SAID STEPS BEING CARRIED OUT AT A TEMPERATURE LESS THAN ABOUT 5*C; AND WHEREIN THE AMOUNT OF SAID ENZYMATIC MATERIAL PRESENT IN SAID COSMETIC COMPOSITION IS FROM ABOUT 0.01 TO ABOUT 1% BY WEIGHT, FURTHER COMPRISING CALCIUM CHLORIDE IN AMOUNT SUFFICIENT TO STABILIZE SAID COSMETIC COMPOSITION AND POLYETHYLENEGLYCOLS HAVING MEAN MOLECULAR WEIGHTS OF 400 AND 4000, IN EQUAL QUANTITIES, THE PH OF THE CMPOSITION BEING ADJUSTED BETWEEN 6.8 AND 8.0 WITH HCL AS A NEUTRALIZING AGENT.
Claim 28
Dependent Claim
77 words
LYOPHILIZATION, SAID STEPS BEING CARRIED OUT AT A TEMPERATURE LESS THAN ABOUT 5*C; AND WHEREIN THE AMOUNT OF SAID ENZYMATIC MATERIAL PRESENT IN SAID COSMETIC COMPOSITION IS FROM ABOUT 0.01 TO ABOUT 1% BY WEIGHT, FURTHER COMPRISING CALCIUM CHLORIDE IN AMOUNT SUFFICIENT TO STABILIZE SAID COSMETIC COMPOSITION AND POLYETHYLENEGLYCOLS HAVING MEAN MOLECULAR WEIGHTS OF 400 AND 4000, IN EQUAL QUANTITIES, THE PH OF THE CMPOSITION BEING ADJUSTED BETWEEN 6.8 AND 8.0 WITH HCL AS A NEUTRALIZING AGENT.
Claim 29
Dependent Claim
77 words
lyophilization, said steps being carried out at a temperature less than about 5*C; and wherein the amount of said enzymatic material present in said cosmetic composition is from about 0.01 to about 1% by weight, further comprising calcium chloride in amount sufficient to stabilize said cosmetic composition and polyethyleneglycols having mean molecular weights of 400 and 4000, in equal quantities, the pH of the composition being adjusted between 6.8 and 8.0 with HCl as a neutralizing agent.
Claim 30
Dependent Claim
77 words
lyophilization, said steps being carried out at a temperature less than about 5*C; and wherein the amount of said enzymatic material present in said cosmetic composition is from about 0.01 to about 1% by weight, further comprising calcium chloride in amount sufficient to stabilize said cosmetic composition and polyethyleneglycols having mean molecular weights of 400 and 4000, in equal quantities, the pH of the composition being adjusted between 6.8 and 8.0 with HCl as a neutralizing agent.
References Cited (Backward Citations)
US2795529A
Cited
US3367836A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-02-03 | 1978-08-05 | 1979-02-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-02-03 | 1982-08-05 | 1983-02-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-02-03 | 1986-08-05 | 1987-02-04 | $7700.00 | Window Closed / Lapsed |