US3864467A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for diagnosis of infectious mononucleosis
Assignee: Orion Oyj
•
Filing: 1972-04-12
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Granted: 1975-02-04
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Expires: 1992-04-12
Abstract
Abstract A method for preparing a storageable absorbent for diagnosis of infectious mononucleosis from blood serum is disclosed. The absorbent is prepared by treating mammal red blood cells with a proteolytic enzyme or neuraminidase to destroy the mononucleosis receptors and the red blood cells are hemolyzed to make a cellfree stroma suspension which does not interfere with the agglutination step of the diagnosis, when mammal blood is added to the mixture of blood serum to be tested and the absorbent on a slide.
Inventors
- 👤 E Juhani Leikola
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 19:40
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/924; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/812
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
97 words
A METHOD FOR DIAGNOSIS OF INFECTIOUS MONONUCLEOSIS COMPRISING FORMING AN ABSORBENT BY TREATING SHEEP OR HORSE RED BLOOD CELLS WITH A PROTEOLYTIC ENZYME OR NEURAMINIDAZE IN ORDER TO DESTROY THE MONONUCLEOSIS RECEPTORS, HEMOLYZING THE RED BLOOD CELLS BY ADDING A HEMOLYZING AGENT, SEPARATING THE RESULTING STROMATA FROM THE HEMOGLOBIN, AND PREPARING A STROMA SUSPENSION FROM THE STROMATA AND DILUTED SALINE WHICH SUSPENSION IS FREE FROM BLOOD CELLS INTERFERING WITH THE DIAGNOSIS; AND MIXING BLOOD SERUM TO BE TESTED, SAID ABSORBENT AND SHEEP OR HORSE BLOOD ON A SLIDE WHEREBY IF MONONUCLEOSIS ANTIBODIES ARE PRESENT AGGLUTINATION TAKES PLACE.
Claim 2
Independent Claim
97 words
A METHOD FOR DIAGNOSIS OF INFECTIOUS MONONUCLEOSIS COMPRISING FORMING AN ABSORBENT BY TREATING SHEEP OR HORSE RED BLOOD CELLS WITH A PROTEOLYTIC ENZYME OR NEURAMINIDAZE IN ORDER TO DESTROY THE MONONUCLEOSIS RECEPTORS, HEMOLYZING THE RED BLOOD CELLS BY ADDING A HEMOLYZING AGENT, SEPARATING THE RESULTING STROMATA FROM THE HEMOGLOBIN, AND PREPARING A STROMA SUSPENSION FROM THE STROMATA AND DILUTED SALINE WHICH SUSPENSION IS FREE FROM BLOOD CELLS INTERFERING WITH THE DIAGNOSIS; AND MIXING BLOOD SERUM TO BE TESTED, SAID ABSORBENT AND SHEEP OR HORSE BLOOD ON A SLIDE WHEREBY IF MONONUCLEOSIS ANTIBODIES ARE PRESENT AGGLUTINATION TAKES PLACE.
Claim 3
Dependent Claim
20 words
The method of claim 1 wherein said sheep or horse blood mixed with said absorbent is in an anticoagulant medium.
Claim 4
Dependent Claim
19 words
The method of claim 1, wherein the red blood cells are washed with diluted saline after the enzyme treatment.
Claim 5
Dependent Claim
16 words
The method of claim 1, wherein an aqueous digitonin solution is used as the hemolyzing agent.
Claim 6
Dependent Claim
14 words
The method of claim 1, wherein a preservative is added to the stromata suspension.
Claim 7
Dependent Claim
13 words
The method of claim 5, wherein sodium azide is used as the preservative.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-02-03 | 1978-08-05 | 1979-02-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-02-03 | 1982-08-05 | 1983-02-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-02-03 | 1986-08-05 | 1987-02-04 | $7700.00 | Window Closed / Lapsed |