US3862300A
Expired / Lapsed
US
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Histological fixative
Assignee: Applied Bioscience
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Filing: 1973-03-07
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Granted: 1975-01-21
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Expires: 1993-03-07
Abstract
Abstract A superior histological fixative is described comprising trichloroacetic acid, zinc chloride and formaldehyde in an aqueous alcoholic solution.
Inventors
- 👤 Paul T Wertlake
- 👤 James S Harrison
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 08:40
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/28; G01N1/30; A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/128; G01N2001/305
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
12 words
The histological fixative of claim 1 wherein the lower alkanol is methanol.
Claim 2
Dependent Claim
22 words
The histological fixative of claim 1 wherein the ratio of zinc chloride to trichloroacetic acid is from about 3:1 to about 3.5:1.
Claim 3
Dependent Claim
18 words
The histological fixative of claim 1 wherein the weight ratio of formaldehyde to trichloroacetic acid is about 9:1.
Claim 4
Dependent Claim
27 words
The histological fixative of claim 1 wherein the lower alkanol is methanol and the volume ratio of methanol to water is from about 1:1.1 to about 1:1.5.
Claim 5
Dependent Claim
19 words
The histological fixative of claim 1 wherein the concentration of said solution is from about 9 to 11% wt/vol.
Claim 6
Dependent Claim
75 words
The histological fixative of claim 1 comprising a 9 to 11% solution wt/vol of a mixture of trichloroacetic acid, zinc chloride and formaldehyde wherein the weight ratio of zinc chloride to trichloriacetic acid is from about 3:1 to about 3.5:1 and the weight ratio of formaldehyde to trichloroacetic acid is from about 8:1 to about 10:1, in aqueous methanol wherein the volume ratio of methanol to total water is from about 1:1.1 to about 1:1.5.
Claim 7
Dependent Claim
48 words
The histological fixative of claim 1 consisting essentially of a 9.8 wt/vol solution of a mixture of 3.3 parts of trichloroacetic acid to 1 part of zinc chlorIde and 9 parts of formaldehyde in aqueous methanol wherein the volume ratio of methanol to total water is about 1:1.3.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-01-20 | 1978-07-22 | 1979-01-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-01-20 | 1982-07-22 | 1983-01-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-01-20 | 1986-07-22 | 1987-01-21 | $7700.00 | Window Closed / Lapsed |