US3858045A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Detection of biological activity
Assignee: Individual
•
Filing: 1973-04-24
•
Granted: 1974-12-31
•
Expires: 1993-04-24
Abstract
Abstract A sample of blood to be tested for the presence of biological activity is added to a suitable growth medium which includes a C14 containing carbon source fermentable to produce gaseous C14O2. After a suitable incubation period, a portion of the gaseous atmosphere above the medium is analyzed for the presence of radioactivity. In the improvement herein described, mammalian cells in the blood sample are lysed in order to reduce background reading when the radioactivity is measured and to provide a better signal-to-noise ratio.
Inventors
- 👤 J Waters
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 07:51
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/16; Y; Y10; Y10S; Y10S435/00; Y10S435/801; Y10S435/8215; Y10S435/822; Y10S435/874; Y10S435/875; Y10S435/882; Y10S435/884
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
136 words
IN THE PROCESS OF DETECTING BIOLOGICAL ACTIVITY IN BLOOD WHEREIN: A. A GROWTH MEDIUM INCLUDING A C14 CONTAINING CARBON SOURCE WHICH IS FERMENTABLE TO PRODUCE CARBON DIOXIDE IS INOCULATED WITH A BLOOD SAMPLE; B. THE INOCULATED SAMPLE IS EXPOSED TO CONDITIONS CONDUCIVE TO THE OCCURENCE OF NORMAL METABOLIC PROCESS FOR A PERIOD OF TIME SUFFICIENT TO CAUSE PRODUCTION OF CARBON DIOXIDE BY THE FERMENTING OF SAID SOURCE; AND C. AT LEAST A PORTION OF THE GASEOUS ATMOSPHERE FROM SAID FERMENTATION IS MEASURED FOR RADIOACTIVITY TO DETERMINE THE PRESENCE OF C14O2, THE IMPROVEMENT COMPRISING LYSING MAMMALIAN CELLS PRESENT IN SAID BLOOD SAMPLE BEFORE THE SAMPLE IS EXPOSED FOR A SUBSTANTIAL PERIOD OF TIME TO CONDITIONS CONDUCIVE TO THE OCCURENCE OF NORMAL METABOLIC PROCESSES, WHEREBY THE BACKGROUND NOISE FOR THE MEASUREMENT OF RADIOACTIVITY OF GASEOUS C14O2 IS REDUCED.
Claim 2
Dependent Claim
136 words
IN THE PROCESS OF DETECTING BIOLOGICAL ACTIVITY IN BLOOD WHEREIN: A. A GROWTH MEDIUM INCLUDING A C14 CONTAINING CARBON SOURCE WHICH IS FERMENTABLE TO PRODUCE CARBON DIOXIDE IS INOCULATED WITH A BLOOD SAMPLE; B. THE INOCULATED SAMPLE IS EXPOSED TO CONDITIONS CONDUCIVE TO THE OCCURENCE OF NORMAL METABOLIC PROCESS FOR A PERIOD OF TIME SUFFICIENT TO CAUSE PRODUCTION OF CARBON DIOXIDE BY THE FERMENTING OF SAID SOURCE; AND C. AT LEAST A PORTION OF THE GASEOUS ATMOSPHERE FROM SAID FERMENTATION IS MEASURED FOR RADIOACTIVITY TO DETERMINE THE PRESENCE OF C14O2, THE IMPROVEMENT COMPRISING LYSING MAMMALIAN CELLS PRESENT IN SAID BLOOD SAMPLE BEFORE THE SAMPLE IS EXPOSED FOR A SUBSTANTIAL PERIOD OF TIME TO CONDITIONS CONDUCIVE TO THE OCCURENCE OF NORMAL METABOLIC PROCESSES, WHEREBY THE BACKGROUND NOISE FOR THE MEASUREMENT OF RADIOACTIVITY OF GASEOUS C14O2 IS REDUCED.
Claim 3
Dependent Claim
23 words
The process of claim 1 wherein the cells are lysed by mixing the blood with water prior to inoculation of the growth medium.
Claim 4
Dependent Claim
30 words
The process of claim 1 wherein the cells are lysed by admixing the blood with a dilute growth medium containing less than about 1 percent by weight of dissolved solids.
Claim 5
Dependent Claim
13 words
The process of claim 1 wherein the cells are lysed with a chemical.
Claim 6
Dependent Claim
16 words
The process of claim 1 wherein the cells are subjected to physical treatment to lyse them.
Claim 7
Dependent Claim
23 words
The process of claim 1 wherein the treatment reduces the background noise due to the blood sample by at least about 30 percent.
Claim 8
Dependent Claim
23 words
The process of claim 1 wherein the treatment reduces the background noise due to the blood sample by at least about 50 percent.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-30 | 1978-07-01 | 1978-12-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-30 | 1982-07-01 | 1982-12-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-30 | 1986-07-01 | 1986-12-31 | $7700.00 | Window Closed / Lapsed |