Patent US2654367A successfully refreshed from Google Patents.
US3857971A
Expired / Lapsed
US
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Ruminant feed additive and method of preparing the same
Assignee: WR Grace and Co Conn
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Filing: 1973-06-25
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Granted: 1974-12-31
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Expires: 1993-06-25
Abstract
Abstract An improved feed additive for administration to ruminant animals having digestive upsets, resulting from imbalances of their rumen microbial population, which occur for various reasons, for example when the ruminants are shifted from a ration which is high in cellulose content to a ration which is high in starch content. The ruminant feed additive comprises rumen microorganisms adapted and cultured in vitro on a medium which is high in starch content. The method of the invention provides an in vitro adaptation and fermentation system for the production of selected rumen microorganisms.
Inventors
- 👤 K Abdo
- 👤 G Cahilly
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 21:52
CPC Classifications
A; A23; A23K; A23K10/00; A23K10/10; A23K10/16; A23K10/18
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
158 words
A METHOD FOR PREPARING A FEED PRODUCT FOR RUMINANTS COMPRISING THE STEPS OF: INTRODUCING VIABLE MICROORGANISMS ISOLATED OR CULTURED FROM THE RUMEN FLYID OF A RUMINANT ANIMAL INTO A NUTRIENT MIXTURE CONSISTING ESSENTIALLY OF AN AQUEOUS SUSPENSION OF A STARCH-CONTAINING CONCENTRATED FEED RATION COMPRISING A MEMBER SELECTED FROM THE GROUP CONSISTING OF GRAIN, ALFALFA, MILO, COTTONSEED MEAL, LINSEED MEAL, SAID SOYBEAN, MEAL, CULTURING SAID SUSPENSION UNDER CARBON DIOXIDE AT A TEMPERATURE IN THE RANGE OF 30*-50*C. THROUGH STAGES SUFFI11 MICROORGANISMS TO SAID FEED RATION, AND COLLECTING SAID ADAPTED RUMEN MICROORGANISMS TO FORM SAID FEED PRODUCT, ADAPTAION BEING DETERMINED BY AT LEAST ONE OF THE FOLLOWING PROCEDURES A AND B; A. A ONE-LITER CULTURE, CULTURED ON 87% CONCENTRATED FEED WITH ARTIFICAL SALVIA MIX FOR 24 HOURS AT 39*C., ANALYZES 0.05 MG/ML L-LACTIC ACID; B. AN 11-LITER FERMENTATION CULTURE, CULTURED ON 87% CONCENTRATE FEED WITH ARTIFICAL SALIVA MIX FOR 8 HOURS AT 39* C., ANALYZES 3.5 MG/ML VOLATILE FATTY ACIDS.
Claim 2
Independent Claim
158 words
A METHOD FOR PREPARING A FEED PRODUCT FOR RUMINANTS COMPRISING THE STEPS OF: INTRODUCING VIABLE MICROORGANISMS ISOLATED OR CULTURED FROM THE RUMEN FLYID OF A RUMINANT ANIMAL INTO A NUTRIENT MIXTURE CONSISTING ESSENTIALLY OF AN AQUEOUS SUSPENSION OF A STARCH-CONTAINING CONCENTRATED FEED RATION COMPRISING A MEMBER SELECTED FROM THE GROUP CONSISTING OF GRAIN, ALFALFA, MILO, COTTONSEED MEAL, LINSEED MEAL, SAID SOYBEAN, MEAL, CULTURING SAID SUSPENSION UNDER CARBON DIOXIDE AT A TEMPERATURE IN THE RANGE OF 30*-50*C. THROUGH STAGES SUFFI11 MICROORGANISMS TO SAID FEED RATION, AND COLLECTING SAID ADAPTED RUMEN MICROORGANISMS TO FORM SAID FEED PRODUCT, ADAPTAION BEING DETERMINED BY AT LEAST ONE OF THE FOLLOWING PROCEDURES A AND B; A. A ONE-LITER CULTURE, CULTURED ON 87% CONCENTRATED FEED WITH ARTIFICAL SALVIA MIX FOR 24 HOURS AT 39*C., ANALYZES 0.05 MG/ML L-LACTIC ACID; B. AN 11-LITER FERMENTATION CULTURE, CULTURED ON 87% CONCENTRATE FEED WITH ARTIFICAL SALIVA MIX FOR 8 HOURS AT 39* C., ANALYZES 3.5 MG/ML VOLATILE FATTY ACIDS.
Claim 3
Dependent Claim
19 words
The method of claim 1 wherein said suspension is cultured at a temperature in the range of 36*-40* C.
Claim 4
Dependent Claim
11 words
The method of Claim 1 wherein said feed ration comprises corn.
Claim 5
Dependent Claim
25 words
The method of claim 1 wherein said feed ration includes a non-protein-nitrogen source selected from the group consisting of urea, ammonia, ammonium sulfate, and biuret.
Claim 6
Dependent Claim
22 words
The method of claim 4 wherein said feed ration comprises B-vitamins, fatty acids containing 4-10 carbon atoms, and magnesium and potassium minerals.
Claim 7
Dependent Claim
109 words
The method of claim 1 wherein said culturing of said viable microorganisms is carried out statically, said static culturing including a multi-step procedure comprising culturing, in a first step, a first sample containing rumen microorganisms, in a suspension of a concentrate feed ration for a period of about 24 hours at a temperature in the range of 36*-40* C., transferring a portion of the thus cultured rumen microorganisms to a second nutrient medium comprising said concentrate feed ration and culturing said portion for another 24-hour period at a temperature in the range of 36*-40* C., and repeating the above procedure until complete adaptation of the rumen microorganisms is obtained.
Claim 8
Dependent Claim
83 words
The method of claim 1 wherein the aqueous suspension of said starch-containing concentrate ration also includes an artificial saliva mix and is diluted by a factor in the range of 0.1 to 25; and after adaptation, there is added a cryo-protective agent selected from the group consisting of glycerol, sucrose, glucose, casein, and whey; following which the mixture is centrifuged and the filtrate removed; the remaining rumen microorganism material is frozen; and thereafter it is thawed and spread over a chosen concentrate feedstuff.
Claim 9
Dependent Claim
25 words
The method of claim 7 wherein the remaining rumen microorganism material is frozen to a temperature in the range of 0* F. to 30* F.
Claim 10
Dependent Claim
19 words
The method of claim 1 in which an artificial saliva mix is added to the nutrient mixture before culturing.
Claim 11
Dependent Claim
79 words
The method of claim 9 wherein the process comprises additionally the following steps: centrifuging the cultured suspension; dEcanting about 9/10 of the centrifuged suspension and discarding it; adding to the residue about 10% based upon the weight of the residue of a cryo-protective agent selected from the group consisting of glycerol, sucrose, glucose, casein, and whey; freezing the mixture; placing the frozen mixture into a drier and pulling a vacuum until the moisture in said mixture is substantially removed.
Claim 12
Dependent Claim
35 words
The method of claim 1 wherein the collected aqueous suspension of adapted rumen microorganisms is mixed with an absorptive inert carrier wherein the weight ratio of suspension; inert carrier is in the range of 0.5-5:1.
Claim 13
Dependent Claim
12 words
The method of claim 11 in which the inert carrier is vermiculite.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-30 | 1978-07-01 | 1978-12-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-30 | 1982-07-01 | 1982-12-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-30 | 1986-07-01 | 1986-12-31 | $7700.00 | Window Closed / Lapsed |