Abstract Process and apparatus for qualitative and quantitative analysis of the amino acid content of a biological sample. The sample is deposited on a cation exchange resin and is then washed with suitable solvents. The amino acids and various cations and organic material with a basic function remain on the resin. The resin is eluted with an acid eluant, and the eluate containing the amino acids is transferred to a reaction vessel where the eluant is removed. The amaino acids are esterified and acylated, and they may then be purified by passage through an anion or cation exchange resin bed. The purified acylated amino acid esters are then transferred to a suitable chromatographic column (e.g., Carbowax as stationary (liquid) phase and diatomaceous silica as solid support) for analysis by gas-liquid chromatographic techniques.
APPARATUS FOR QUALITATIVELY AND QUANTITATIVELY DETERMINING THE ANIMO ACID CONTENTS OF A SAMPLE, WHICH APPARATUS COMPRISES: A REACTION VESSEL FOR HOLDING A GROUP OF AMINO ACIDS FROM A SAMPLE, MEANS FOR SUPPLYING TO SAID VESSEL IN SERIAL ORDER (A) AN AZEOTROPING AGENT, (B) AN ESTERIFYING REAGENT, AND (C) AXEOTROPING AGENT TO CONVERT SAID AMINO ACIDS TO THE NACYL AMINO ACID ALKYL ESTER DERIVATIVES THEREOF, MEANS FOR HEATING SAID REACTION VESSEL TO REMOVE SOLVENTS AND WATER, MEANS FOR SUPPLYING TO SAID VESSEL A CARRIER SOLVENT FOR SAID ENTIRE N-ACYL AMINO ACID ALKYL ESTER MIXTURE, HEATING CHAMBER MEANS TO WHICH SAID MIXTURE IS TRANSFERRED FROM SAID REACTION VESSEL, MEANS FOR CONNECTING SAID CHAMBER MEANS TO WASTE WHILE SAID CARRIER SOLVENT IS VAPORIZED, MEANS FOR THEREAFTER CONNECTING SAID CHAMBER MEANS TO THE INLET OF A GAS CHROMATOGRAPHIC DEVICE AND TO A SOURCE OF CARRIER GAS FOR TRANSFERRING SAID AMINO ACID DERIVATIVES THERETO IN THE GASEOUS STATE, AND A CONTROL DEVICE CONNECTED TO EACH OF SAID ABOVE-MENTIONED MEANS FOR AUTOMATICALLY EFFECTING SEQUENTIAL TIMED OPERATION OF EACH OF SAID SAID ABOVE-MENTIONED MEANS.
Claim 2
Dependent Claim
177 words
APPARATUS FOR QUALITATIVELY AND QUANTITATIVELY DETERMINING THE ANIMO ACID CONTENTS OF A SAMPLE, WHICH APPARATUS COMPRISES: A REACTION VESSEL FOR HOLDING A GROUP OF AMINO ACIDS FROM A SAMPLE, MEANS FOR SUPPLYING TO SAID VESSEL IN SERIAL ORDER (A) AN AZEOTROPING AGENT, (B) AN ESTERIFYING REAGENT, AND (C) AXEOTROPING AGENT TO CONVERT SAID AMINO ACIDS TO THE NACYL AMINO ACID ALKYL ESTER DERIVATIVES THEREOF, MEANS FOR HEATING SAID REACTION VESSEL TO REMOVE SOLVENTS AND WATER, MEANS FOR SUPPLYING TO SAID VESSEL A CARRIER SOLVENT FOR SAID ENTIRE N-ACYL AMINO ACID ALKYL ESTER MIXTURE, HEATING CHAMBER MEANS TO WHICH SAID MIXTURE IS TRANSFERRED FROM SAID REACTION VESSEL, MEANS FOR CONNECTING SAID CHAMBER MEANS TO WASTE WHILE SAID CARRIER SOLVENT IS VAPORIZED, MEANS FOR THEREAFTER CONNECTING SAID CHAMBER MEANS TO THE INLET OF A GAS CHROMATOGRAPHIC DEVICE AND TO A SOURCE OF CARRIER GAS FOR TRANSFERRING SAID AMINO ACID DERIVATIVES THERETO IN THE GASEOUS STATE, AND A CONTROL DEVICE CONNECTED TO EACH OF SAID ABOVE-MENTIONED MEANS FOR AUTOMATICALLY EFFECTING SEQUENTIAL TIMED OPERATION OF EACH OF SAID SAID ABOVE-MENTIONED MEANS.
Claim 3
Dependent Claim
37 words
Apparatus in accordance with claim 1 wherein a gas-liquid chromatographic device is provided having a column containing between about 0.25% and 1% Carbowax as the stationary liquid phase, based upon total weight of Carbowax and inert filler.