Abstract 1. A DIPOLE-ION-EXCHANGE CHROMATOGRAPHIC METHOD OF SEPARATING DIFFERENT TYPES OF PROTEINS AND PEPTIDES FROM EACH OTHER COMPRISING PASSING A SOLUTION OF POLYELECTROLYTES SELECTED FROM THE GROUP CONSISTING OF PROTEINS AND PEPTIDES, THROUGH A BED OF AN AMPHOTERIC ADSORBENT, SAID ADSORBENT COMPRISING AN INSOLUBLE, ORGANIC HYDROXYLCONTAINING, HYDROPHILIC POLYMER CONTAINING BOTH BASIC, NITROGEN-CONTAINING GROUPS AND CARBOXYLATE GROUPS IN THE SAME MOLECULE, WHERE THE PORPORTION BETWEEN THE BASIC AND THE ACID GROUPS IS SIMPLE AND STOICHIOMETRIC, SAID POLYMER BEING THE REACTION PRODUCT OF AN AMINO ACID OR AN AMINO ACID DERIVATIVE AND AN INSOLUBLE ORGANIC HYDROXYL-CONTAINING HYDROPHILIC CARBOHYDRATE POLYMER, WHEREBY SAID POLYELECTROLYTES WILL MIGRATE AT DIFFERENT SPEEDS DEPENDING ON THE STRENGTH OF THE RECIPROCAL INTERACTION BETWEEN IN POLYELECTROLYTE AND THE SUBSTITUENTS IN SAID ADSORBENT, AND ARE SELCTIVELY ADSORBED BY SAID ADSORBENT.
A DIPOLE-ION-EXCHANGE CHROMATOGRAPHIC METHOD OF SEPARATING DIFFERENT TYPES OF PROTEINS AND PEPTIDES FROM EACH OTHER COMPRISING PASSING A SOLUTION OF POLYELECTROLYTES SELECTED FROM THE GROUP CONSISTING OF PROTEINS AND PEPTIDES, THROUGH A BED OF AN AMPHOTERIC ADSORBENT, SAID ADSORBENT COMPRISING AN INSOLUBLE, ORGANIC HYDROXYLCONTAINING, HYDROPHILIC POLYMER CONTAINING BOTH BASIC, NITROGEN-CONTAINING GROUPS AND CARBOXYLATE GROUPS IN THE SAME MOLECULE, WHERE THE PORPORTION BETWEEN THE BASIC AND THE ACID GROUPS IS SIMPLE AND STOICHIOMETRIC, SAID POLYMER BEING THE REACTION PRODUCT OF AN AMINO ACID OR AN AMINO ACID DERIVATIVE AND AN INSOLUBLE ORGANIC HYDROXYL-CONTAINING HYDROPHILIC CARBOHYDRATE POLYMER, WHEREBY SAID POLYELECTROLYTES WILL MIGRATE AT DIFFERENT SPEEDS DEPENDING ON THE STRENGTH OF THE RECIPROCAL INTERACTION BETWEEN IN POLYELECTROLYTE AND THE SUBSTITUENTS IN SAID ADSORBENT, AND ARE SELCTIVELY ADSORBED BY SAID ADSORBENT.
Claim 2
Dependent Claim
127 words
A DIPOLE-ION-EXCHANGE CHROMATOGRAPHIC METHOD OF SEPARATING DIFFERENT TYPES OF PROTEINS AND PEPTIDES FROM EACH OTHER COMPRISING PASSING A SOLUTION OF POLYELECTROLYTES SELECTED FROM THE GROUP CONSISTING OF PROTEINS AND PEPTIDES, THROUGH A BED OF AN AMPHOTERIC ADSORBENT, SAID ADSORBENT COMPRISING AN INSOLUBLE, ORGANIC HYDROXYLCONTAINING, HYDROPHILIC POLYMER CONTAINING BOTH BASIC, NITROGEN-CONTAINING GROUPS AND CARBOXYLATE GROUPS IN THE SAME MOLECULE, WHERE THE PORPORTION BETWEEN THE BASIC AND THE ACID GROUPS IS SIMPLE AND STOICHIOMETRIC, SAID POLYMER BEING THE REACTION PRODUCT OF AN AMINO ACID OR AN AMINO ACID DERIVATIVE AND AN INSOLUBLE ORGANIC HYDROXYL-CONTAINING HYDROPHILIC CARBOHYDRATE POLYMER, WHEREBY SAID POLYELECTROLYTES WILL MIGRATE AT DIFFERENT SPEEDS DEPENDING ON THE STRENGTH OF THE RECIPROCAL INTERACTION BETWEEN IN POLYELECTROLYTE AND THE SUBSTITUENTS IN SAID ADSORBENT, AND ARE SELCTIVELY ADSORBED BY SAID ADSORBENT.