US3847737A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Inactivation of myxoviruses and method of preparing a vaccine therefrom
Assignee: Individual
•
Filing: 1968-06-11
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Granted: 1974-11-12
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Expires: 1988-06-11
Abstract
Abstract A method for inactivating myxoviruses without significant loss of antigenic activity, which comprises contacting the myxoviruses in an aqueous suspension at a pH between 6 and 8 in the presence of at least about 0.05 percent w/v of a surface active agent selected from the class of polyoxyethylene ester of partial oleic acid, polyoxyethylene ester of lauric acid, polyoxyethylene ester of palmitic acid, and polyoxyethylene ester of stearic acid, with an organic solvent selected from the class consisting of chlorinated lower hydrocarbon having from 2 to 5 carbon atoms and chlorinated and fluorinated lower hydrocarbon having from 2 to 5 carbon atoms, the solvent being liquid at room temperature.
Inventors
- 👤 A Kanarek
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 21:57
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/155; A61K39/165; A61K39/145; C; C12; C12N; C12N7/00; A61K2039/51; A61K2039/525; A61K2039/5252; A61K2039/555; A61K2039/55505; C12N2760/00; C12N2760/00011; C12N2760/16011; C12N2760/16111; C12N2760/16161; C12N2760/16163; C12N2760/16211; C12N2760/16261; C12N2760/16263; C12N2760/18011; C12N2760/18411; C12N2760/18434; C12N2760/18461; C12N2760/18463; C12N2760/18811; C12N2760/18861; C12N2760/18863
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
152 words
A METHOD FOR INACTIVATING MYXOVIRUSES WITHOUT SIGNIFICANT LOSS OF ANTIGENIC ACTIVITY, WHICH COMPRISES CONTACTING THE MYXOVIRUSES IN AN AQUEOUS SUSPENSION AT A PH BETWEEN PH 6 AND PH 8 IN THE SPRESENCE OF AT LEAST ABOUT 0.05 PERCENT W/V OF A SURFACE ACTIVE AGENT SELECTED FROM THE CLASS CONSISTING OF POLYOXYETHYLENE SORBITAN MONO-OLEATE, POLYOXYETHYLENE ETHER OF PARTIAL LAURIC ACID ESTER, POLYOXYETHYLENE ETHER OF PALMITIC ACID ESTER, AND POLYOXYETHYLENE ETHER OF STERIC ACID ESTER WHICH HAVE MOLECULAR WEIGHTS IN THE RANGE OF 1,200 TO 1,320, WITH AN ORGANIC SOLVENT SELECTED FROM THE CLASS CONSISTING OF CHLORINATED LOWER HYDROCARBON HAVING FROM 2 TO 5 CARBON ATOMS AND CHLORINATED AND FLUORINATED LOWER HYDROCARBON HAVING FROM 2 TO 5 CARBON ATOMS, THE SOLVENT BEING LIQUID AT ROOM TEMPERATURE SO AS TO FORM AN ORGANIC LAYER AND AN AQUEOUS LAYER, AND SEPARATING THE AQUEOUS LAYER FROM THE ORGANIC LAYER AFTER A MIXING TIME OF ABOUT 1 HOUR.
Claim 2
Independent Claim
152 words
A METHOD FOR INACTIVATING MYXOVIRUSES WITHOUT SIGNIFICANT LOSS OF ANTIGENIC ACTIVITY, WHICH COMPRISES CONTACTING THE MYXOVIRUSES IN AN AQUEOUS SUSPENSION AT A PH BETWEEN PH 6 AND PH 8 IN THE SPRESENCE OF AT LEAST ABOUT 0.05 PERCENT W/V OF A SURFACE ACTIVE AGENT SELECTED FROM THE CLASS CONSISTING OF POLYOXYETHYLENE SORBITAN MONO-OLEATE, POLYOXYETHYLENE ETHER OF PARTIAL LAURIC ACID ESTER, POLYOXYETHYLENE ETHER OF PALMITIC ACID ESTER, AND POLYOXYETHYLENE ETHER OF STERIC ACID ESTER WHICH HAVE MOLECULAR WEIGHTS IN THE RANGE OF 1,200 TO 1,320, WITH AN ORGANIC SOLVENT SELECTED FROM THE CLASS CONSISTING OF CHLORINATED LOWER HYDROCARBON HAVING FROM 2 TO 5 CARBON ATOMS AND CHLORINATED AND FLUORINATED LOWER HYDROCARBON HAVING FROM 2 TO 5 CARBON ATOMS, THE SOLVENT BEING LIQUID AT ROOM TEMPERATURE SO AS TO FORM AN ORGANIC LAYER AND AN AQUEOUS LAYER, AND SEPARATING THE AQUEOUS LAYER FROM THE ORGANIC LAYER AFTER A MIXING TIME OF ABOUT 1 HOUR.
Claim 3
Dependent Claim
22 words
A method according to claim 1, in which the concentration of the surface active agent is between 0.05 to 0.2 percent w/v.
Claim 4
Dependent Claim
18 words
A mehod according to claim 1, in which the pH of the suspension is between 6.5 and 7.5.
Claim 5
Dependent Claim
25 words
A method according to claim 1, in which the organic solvent is selected from the class consisting of trichloro-ethylene, tetrachloro-ethylene, 1,1,2-trichloro-1,2,2-trifluoro-ethane, 1, 1,1,3,3,3-hexachloro-propane and hexachloro-buta1,3-diene.
Claim 6
Independent Claim
121 words
A method for inactivating myxoviruses without significant loss of antigenic activity, which comprises contacting the myxoviruses in an aqueous suspension at a pH between pH 6 and pH 8 in the presence of at least about 0.05 percent w/v of a surface active agent selected for the class consisting of polyoxyethylene sorbitan mono-oleate, polyoxyethylene ether of palmitic acid, ester, polyoxyethylene ether of partial lauric acid ester, and polyoxyethylene ether of stearic acid ester which have molecular weights in the range of 1,220 to 1,320, with tetrachloro-methane, the solvent being liquid at room temperature, so as to form an organic layer and an aqueous layer, and separating the aqueous layer from the organic layer after a mixing time of about 1 hour.
Claim 7
Independent Claim
121 words
A method for inactivating myxoviruses without significant loss of antigenic activity, which comprises contacting the myxoviruses in an aqueous suspension at a pH between pH 6 and pH 8 in the presence of at least about 0.05 percent w/v of a surface active agent selected for the class consisting of polyoxyethylene sorbitan mono-oleate, polyoxyethylene ether of palmitic acid, ester, polyoxyethylene ether of partial lauric acid ester, and polyoxyethylene ether of stearic acid ester which have molecular weights in the range of 1,220 to 1,320, with tetrachloro-methane, the solvent being liquid at room temperature, so as to form an organic layer and an aqueous layer, and separating the aqueous layer from the organic layer after a mixing time of about 1 hour.
Claim 8
Dependent Claim
22 words
A method according to claim 5, in which the concentration of the surface active agent is between 0.05 to 0.2 percent w/v.
Claim 9
Dependent Claim
18 words
A method according to claim 5, in which the pH of the suspension is between 6.5 and 7.5.
References Cited (Backward Citations)
US2798836A
Cited
US3269912A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-11-11 | 1978-05-13 | 1978-11-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-11-11 | 1982-05-13 | 1982-11-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-11-11 | 1986-05-13 | 1986-11-12 | $7700.00 | Window Closed / Lapsed |