US3847545A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic test for sickle-cell
Assignee: Baxter International Inc
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Filing: 1972-05-18
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Granted: 1974-11-12
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Expires: 1992-05-18
Abstract
Abstract A microcapillary method for the diagnosis of sickle-cell disease in which a sample of unclotted blood is mixed with a reagent comprising phosphate buffer containing a non-ionic surfactant having high water solubility, a hemolyzing agent and a reducing agent, and the reaction results observed macroscopically for turbidity and then a microcapillary sample thereof observed macroscopically for color characteristics.
Inventors
- 👤 E Shanbrom
- 👤 A Louderback
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 09:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/5005; G01N33/5091; G01N33/72; G01N33/721
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
93 words
A REAGENT FOR THE DIAGNOSIS OF SICKEL-CELL DISEASE COMPRISING A MIXTURE OF AN AQUEOUS PHOSPHATE BUFFER SOLUTION HAVING A MOLARITY OF FROM ABOUT 2 TO ABOUT 2.5 AND A PH OF FROM ABOUT 7 TO ABOUT 7.2, A HEMOLYZING GENT AND A REDUCING AGENT, SAID BUFFER SOLUTION ADDITIONAALLY CONTAINING FROM ABOUT 0.1 TO ABOUT ONE MIL. PER LITER OF A NON-IONIC SURFACTANT HAVING A HIGH WATER SOLUBILITY AND BEING SELECTED FROM THE GROUP CONSISTING OF SUBSTANCES HAVING THE GENERAL FORMULA RC6H4(OCH2H4)NOH WHEREIN R IS OCTYL OR NONYL AND N IS AT LEAST 3.
Claim 2
Dependent Claim
93 words
A REAGENT FOR THE DIAGNOSIS OF SICKEL-CELL DISEASE COMPRISING A MIXTURE OF AN AQUEOUS PHOSPHATE BUFFER SOLUTION HAVING A MOLARITY OF FROM ABOUT 2 TO ABOUT 2.5 AND A PH OF FROM ABOUT 7 TO ABOUT 7.2, A HEMOLYZING GENT AND A REDUCING AGENT, SAID BUFFER SOLUTION ADDITIONAALLY CONTAINING FROM ABOUT 0.1 TO ABOUT ONE MIL. PER LITER OF A NON-IONIC SURFACTANT HAVING A HIGH WATER SOLUBILITY AND BEING SELECTED FROM THE GROUP CONSISTING OF SUBSTANCES HAVING THE GENERAL FORMULA RC6H4(OCH2H4)NOH WHEREIN R IS OCTYL OR NONYL AND N IS AT LEAST 3.
Claim 3
Dependent Claim
15 words
The reagent of claim 1 in which the non-ionic surfactant is octyl phenoxy polyethoxy ethanol.
Claim 4
Dependent Claim
12 words
The reagent of claim 1 in which the hemolyzing agent is saponin.
Claim 5
Dependent Claim
13 words
The reagent of claim 1 in which the reducing agent is sodium dithionate.
Claim 6
Dependent Claim
15 words
The reagent of claim 1 in which the phosphate buffer is about 2.25 molar KHPO4/K2HPO4.
Claim 7
Dependent Claim
29 words
The reagent of claim 1 in which the mixture contains about 135 grams of KH2PO4, about 237 grams of K2HPO4, and about 0.4 ml. of non-ionic surfactant per liter.
Claim 8
Dependent Claim
101 words
A method for the rapid differentiation of sickle-cell anemia and sickle-cell trait comprising admixing a sample of unclotted blood with a predetermined amount of the reagent of claim 1 and observing macroscopically for turbidity in the solution as indicative of presence of HbS, then withdrawing a microcapillary sample of the turbid solution and centrifuging in a hematocrit centrifuge and observing macroscopically for color characteristics in the solution in which a pink solution with a dark red clump at the surface is indicative of sickle-cell trait and a yellow solution with a red clump at the surface is indicative of sickle-cell anemia.
Claim 9
Dependent Claim
28 words
The method of claim 7 in which the blood sample and reagent are admixed in proportions of about 100 microliters of blood with about 2 milliliters of reagent.
References Cited (Backward Citations)
US3492095A
Cited
US3607695A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-11-11 | 1978-05-13 | 1978-11-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-11-11 | 1982-05-13 | 1982-11-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-11-11 | 1986-05-13 | 1986-11-12 | $7700.00 | Window Closed / Lapsed |