Anti-peptic ulcer substance from corydalis tubers

Claim 2

AN ANTI-PEPTIC UCLER ACTIVE SUBSTANCE PREPARED BY THE PROCESS COMPRISING (A) EXTRACTING A TUBER OF THE GENUS CORYDALIS WITH A SOLVENT SELECTED FROM THE GROUP CONSISTING OF WATER, METANOL, ETHANOL, PROPANOL AND MIXTURES THEREOF, (B) CONTAINING THE RESULTING EXTRACT BY VACUUM DISTILLATION OF SOLVENT THEREFROM, (C) ACIDIFYING THE CONCENTRATED EXTRACT TO A PH OF 1 TO 4 BY ADDING THERETO ACETIC ACID, TARTARIC ACID, CITRIC ACID, HYDROCHLORIC ACID, HYDROCHLORIC ACID AND SULFURIC ACID, (D) EXTRACTING THE ACIDIFIED AQUEOUS SOLUTION OF THE EXTRACT WITH A HYDROCARBON SOLVENT SELECTED FROM THE GROUP CONSISTING OF PETROLEUM ETHER AND PETROLEUM BENZIN TO REMOVE THE FATTY SUBSTANCE THEREFROM, (E) RAISING THE PH OF THE DEFATTED AQUEOUS ACIDIC PHASE TO 7.5 TO 12 BY ADDING THERETO A BASE SELECTED FROM THE GROUP CONSISTING OF AMMONIA, ALKALI METAL HYDROXIDE. ALKALI METAL CARBONATE AND ALKALI METAL BICARBONATE, (F) EXTRACTING THE RESULTING AQUEOUS ALKALINE SOLUTION WITH CHLOROFORM, DICHLOROMETHANE, TRICHLOMETHANE OR CARBON TETRACHLORIDE, SEPARATING THE HALOHYDROCARBON PHASE FROM THE AQUEOUS PHASE BY VACUUM DISTILLATION AND RECOVERING SAID ACTIVE SUBSTANCE.

Claim 3

A process for preparation of an anti-peptic ulcer active substance which comprises extracting tubers of the genus Corydalis with a solvent selected from the group consisting of water, methanol, ethanol, propanol and mixtures thereof, concentrating the extract by distillation of solvent therefrom under subatmospheric pressure, acidifying the concentrated extract to a pH of 1 to 4 by addition thereto of a dilute acid selected from the group consisting of acetic acid, tartaric acid, citric acid, hydrochloric acid, hydrobromic acid and sulphuric acid, contacting the acidified extract with a hydrocarbon solvent selected from the group consisting of petroleum ether and petroleum benzin to remove fatty substances therefrom, raising the pH of the defatted extract to 7.5 - 12 by adding thereto ammonia, contacting the defatted alkaline solution with a halogenated hydrocarbon selected from the group consisting of chloroform, dichloromethane, trichloroethane and carbon tetrachloride to extract the active substance therefrom, separating the extract from the aqueous solution and distilling the resulting extract to remove the halogenated hydrocarbon solvent from the active substance, and recovering the active substance.

Claim 4

A process for preparation of an anti-peptic ulcer active substance which comprises extracting tubers of the genus Corydalis with a solvent selected from the group consisting of water, methanol, ethanol, propanol and mixtures thereof, concentrating the extract by distillation of solvent therefrom under subatmospheric pressure, acidifying the concentrated extract to a pH of 1 to 4 by addition thereto of a dilute acid selected from the group consisting of acetic acid, tartaric acid, citric acid, hydrochloric acid, hydrobromic acid and sulphuric acid, contacting the acidified extract with a hydrocarbon solvent selected from the group consisting of petroleum ether and petroleum benzin to remove fatty substances therefrom, raising the pH of the defatted extract to 7.5 - 12 by adding thereto ammonia, contacting the defatted alkaline solution with a halogenated hydrocarbon selected from the group consisting of chloroform, dichloromethane, trichloroethane and carbon tetrachloride to extract the active substance therefrom, separating the extract from the aqueous solution and distilling the resulting extract to remove the halogenated hydrocarbon solvent from the active substance, and recovering the active substance.