Antibiotics a201a and a201b and process for the production thereof

Claim 2

THE ANTIBIOTIC SUBSTANCE A-201A, A WHITE CRYSTALLINE COMPOUND SOLUBLE IN ALCHOLS, ACETONE, CHLOROFORM AND ETHYL ACETATE AND INSOLUBLE IN WATER, HYDROCARBONS , AND ETHER; MELTING AT ABOUT 170*-72*C; HAVING AN COMPOSITION OF 55.20 PERCENT CARBON, 6.72 PERCENT HYDROGEN, 10.45 PERCENT NITROGEN, AND 28-05 PERCENT OXYGEN; HAS AN APPARENT MOLECULAR WEIGHT DETERMINED BY MASS SPECTROSCOPY OF 693; HAVING AN ULTRAVIOLET ABSORPTION MAXIMUM AT ABOUT 208 MP, WITH AN EXTINCTION COEFFICIENT, E1CM 1% OF 535, AND AN ABSORPTION MAXIMUM AT ABOUT 275MP, WITH AN EXTINCTION COEFFICIENT, E1CM.1% OF 490; AND HAVING THE FOLLOWING DISTINGUISHABLE BANDS IN THE INFRARED ABSORPTION SPECTRUM WHEN DISSOLVED IN CHOROFORM: 2.96 (BROAD), 3.45, 3.53, 5.89, 6.06, 6.33, 6.40, 6.67, 6.92, 7.06, 7.15, 7.28, 7.46, 7.60, 7.76, 7.92, 8.10, 8.30, 8.60, 8.87, 9.06, 9.14, 9.32, 9.54, 10.12, 10.23, 10.59, 11.01, 11.30, 11.60, AND 12.00 MICRONS.

Claim 3

The antibiotic substance A-201B, an oil at 25*C., soluble in alcohols, acetone, chloroform and ethyl acetate, and insoluble in water, hydrocarbons, and ether; having an composition of 65.41 percent carbon, 9.03 percent hydrogen, 5.76 percent nitrogen, and 17.78 percent oxygen; has an apparent molecular weight determined by the vapor pressure osmotic method of 417; having an ultraviolet absorption maximum at about 222 m Mu ., with an extinction coefficient, E1cm.1% of 515, an absorption maximum at about 242 m Mu ., with an extinction coefficient, E1cm.1% of 405, an absorption maximum at about 327 m Mu ., with an extinction of coefficient, E1cm.1% of 150, and an absorption maximum at about 340 m Mu ., with an extinction coefficient, E1cm.1% of 225; and having the following distinguishable bands in the infrared absorption spectrum when dissolved in chloroform: 2.77, 3.05, 3.45, 3.51, 5.90, 6.05, 6.20, 6.60, 6.86, 6.95, 7.10, 7.30, 7.40, 8.30, 8.90, 9.00, 9.64, 10.10, 11.20, 12.30, and 15.23 microns.

Claim 4

The antibiotic substance A-201B, an oil at 25*C., soluble in alcohols, acetone, chloroform and ethyl acetate, and insoluble in water, hydrocarbons, and ether; having an composition of 65.41 percent carbon, 9.03 percent hydrogen, 5.76 percent nitrogen, and 17.78 percent oxygen; has an apparent molecular weight determined by the vapor pressure osmotic method of 417; having an ultraviolet absorption maximum at about 222 m Mu ., with an extinction coefficient, E1cm.1% of 515, an absorption maximum at about 242 m Mu ., with an extinction coefficient, E1cm.1% of 405, an absorption maximum at about 327 m Mu ., with an extinction of coefficient, E1cm.1% of 150, and an absorption maximum at about 340 m Mu ., with an extinction coefficient, E1cm.1% of 225; and having the following distinguishable bands in the infrared absorption spectrum when dissolved in chloroform: 2.77, 3.05, 3.45, 3.51, 5.90, 6.05, 6.20, 6.60, 6.86, 6.95, 7.10, 7.30, 7.40, 8.30, 8.90, 9.00, 9.64, 10.10, 11.20, 12.30, and 15.23 microns.

Claim 5

A method of producing the antibiotic substance A-201A as defined in claim 1 and antibiotic substance A-201B as defined in claim 2 which comprises cultivating the organism Streptomyces capreolus, strain NRRL 3817, in a liquid culture medium containing assimilable sources of carbon, nitrogen and inorganic salts, under submerged aerobic conditions until a substantial amount of antibiotic activity is imparted by said organism to said culture medium, and separating said antibiotic activity therefrom.