US3834991A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Colorimetric assay for lysozyme
Assignee: Warner Lambert Co LLC
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Filing: 1972-05-23
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Granted: 1974-09-10
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Expires: 1992-05-23
Abstract
Abstract A SUSPENSION OF DYED MICROCOCCUS LYSODEIKTICUS CELLS IS USED AS THE SUBSTRATE IN A NOVEL, COLORIMETRIC ASSAY FOR LYSOZYME IN BODY FLUIDS. TO OBTAIN THE SUBSTRATE, DRIED MICROCOCCUS LYSODEIKTICUS CELLS ARE TREATED WITH A REACTIVE DYE, AND ALL UNREACTED DYE IS REMOVED UPON REPEATED WASHING WITH WATER. A BUFFERED, AQUEOUS SUSPENSION OF THE DYED CELLS IS INCUBATED WITH A FLUID TEST SAMPLE CONTAINING AN UNKNOWN QUANTITY OF LYSOZYME. CELL LYSIS YIELDS A SOLUTION OF DYED FRAGMENTS OF MICROCOCCUS LYSODEIKITICUS CELLS, THE ABSORBANCE OF WHICH PROVIDES A MEASURE OF LYSOZYME CONCENTRATION IN THE TEST SAMPLE.
Inventors
- 👤 R Megraw
- 👤 J Woodson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 04:00
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/305; G01N2333/90; G01N2333/914; G01N2333/924; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/859; Y10S435/862
Claims Analysis
Total Claims: 0
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References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-09-09 | 1978-03-11 | 1978-09-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-09-09 | 1982-03-11 | 1982-09-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-09-09 | 1986-03-11 | 1986-09-10 | $7700.00 | Window Closed / Lapsed |