US3832801A
Expired / Lapsed
US
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Fertile interspecific hybridization cell fusion for higher plants
Assignee: United States Atomic Energy Commission
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Filing: 1973-05-08
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Granted: 1974-09-03
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Expires: 1993-05-08
Abstract
Abstract Interspecific plant hybrids are produced by parasexual procedures. Protoplasts of separate plant species are isolated, fused, and induced to regenerate into plants. The somatic hybrids are recovered from a mixed population of parental and fused protoplasts by a selective screening method that relies on differential growth of the hybrid on defined culture media. The biochemical and morphological characteristics of the somatically produced hybrid are identical to those of the sexually produced amphiploid.
Inventors
- 👤 P Carlson
- 👤 H Smith
- 👤 R Dearing
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 05:59
CPC Classifications
A; A01; A01H; A01H1/00; A01H1/02; A01H1/021; A01H4/00; C; C12; C12N; C12N5/00; C12N5/10; C12N5/12; C12N5/14
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
41 words
The method of claim 1 in which differentiation is promoted by grafting calli having rudimentary shoots and leaves in culture onto the cut stem surface of a young plant of either parental species followed by propagation to obtain such further differentiation.
Claim 2
Independent Claim
154 words
The method for the production of fertile interspecific hybrid plants comprising the steps of: a. isolating a first cell-strain of the N. glauca plant species; b. isolating a second cell-strain of the N. langsdorffi plant species; c. preparing wall-less cells of said first and second cell-strains; d. causing some of the wall-less cells of said first and second cell-strain to fuse together to form a mixed population of fused cells; e. growing said fused cells in contact with a culture medium lacking in specific culture-medium additives essential to the growth of said first and second cell-strains under conditions suitable for fused cell replication for a period of time sufficient to form cell hybrid colonies from fused hybrid cells; f. isolating said cell hybrid colonies; and g. growing said cell hybrid colonies under conditions to promote differentiation to form hybrid plants having a ploidy higher than the ploidy of either one of the plant species.
References Cited (Backward Citations)
NL7101939A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-09-02 | 1978-03-04 | 1978-09-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-09-02 | 1982-03-04 | 1982-09-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-09-02 | 1986-03-04 | 1986-09-03 | $7700.00 | Window Closed / Lapsed |