US3819486A
Expired / Lapsed
US
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Process for the preparation of pepsin inhibitors
Assignee: Eisai Co Ltd
•
Filing: 1973-01-12
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Granted: 1974-06-25
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Expires: 1993-01-12
Abstract
Abstract New substances named ''''substance S-PI'''' and ''''substance Me SPI'''' are concerned which are effective as pepsin inhibitor, the latter substance being methyl ester of the former. The substance S-PI is prepared by fermentation of a new strain of microorganism which has been isolated from soil by the present inventors and named the Streptomyces naniwaensis (EF 44-201), whereas the substance Me S-PI is prepared by methyl esterfying the former substance. A specimen of the Streptomyces naniwaensis (EF 44-201) has been deposited to the American Type Culture Collection under the assigned Number ATCC 21689.
Inventors
- 👤 S Murao
- 👤 S Satoi
- 👤 N Kuwana
- 👤 M Fukumura
- 👤 T Kawamura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 23:31
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/16; A61K38/55; A61K38/57; C; C07; C07K; C07K14/00; C07K14/81; C07K14/8107; C07K14/8142; C12; C12P; C12P21/00; C12P21/02; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
42 words
A process as claimed in claim 1 wherein the fermentation is carried out in a nutrient medium utilizing submerged culture, stationary culture or surface culture, at a temperature of from about 20*C to about 35*C, for from about 10 to 96 hours.
Claim 2
Dependent Claim
59 words
A process as claimed in claim 2 wherein the nutrient medium contains a nitrogen source selected from the group consisting of soybean meal, corn steep liquor, peptone, yeast extract, meat extract, dry yeast, inorganic nitrates, ammonium salts, and mixtures thereof, inorganic salts; and a carbon source selected from the group consisting of starch hydrolyzate, glucose molasses, and mistures thereof.
Claim 3
Dependent Claim
24 words
A process as claimed in claim 1 wherein recovery is effected by absorption on active carbon followed by elution with a water miscible solvent.
Claim 4
Dependent Claim
15 words
A process as in claim 1 wherein recovery is effected by precipitation with ammonium sulfate.
Claim 5
Dependent Claim
15 words
A process as in claim 4 wherein the recovered substance is purified by column chromatography.
Claim 6
Dependent Claim
15 words
A process as in claim 5 wherein the recovered substance is purified by column chromatography.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-06-24 | 1977-12-24 | 1978-06-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-06-24 | 1981-12-24 | 1982-06-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-06-24 | 1985-12-24 | 1986-06-25 | $7700.00 | Window Closed / Lapsed |