US3816262A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reagent formulations for assaying biological specimens amd methods of preparing and using same
Assignee: Mallinckrodt Chemical Works
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Filing: 1971-11-19
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Granted: 1974-06-11
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Expires: 1991-11-19
Abstract
Abstract A solid, water-soluble substantially anhydrous, storage-stable reagent formulation for use in conducting a clinical diagnostic test on a biological specimen is provided. The reagent formulation comprises a mixture containing a reagent capable of participating in a test reaction to effect a measurable change in a test system; and a nitrogen bearing polyoxyalkylene nonionic surfactant. The surfactant has a structure corresponding to that obtained when ethylene diamine is reacted sequentially with propylene oxide and ethylene oxide in the presence of a catalyst. The polyoxypropylene chains of said surfactant have an average molecular weight of between about 750 and about 6,750. Methods of preparing the reagent formulations and methods of using them to conduct clinical diagnostic tests are also provided.
Inventors
- 👤 A Monte
- 👤 C Chiang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 23:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; C; C12; C12Q; C12Q1/00; C12Q1/008; G01N33/72; G01N33/721
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
12 words
removing the solvent to form a substantially anhydrous, free-flowing, water-soluble, granular solid.
Claim 2
Dependent Claim
12 words
removing the solvent to form a substantially anhydrous, free-flowing, water-soluble, granular solid.
Claim 3
Dependent Claim
130 words
a substrate reagent formulation containing: a. lithium lactate; b. tris-(hydroxymethyl)-aminomethane; c. sodium bicarbonate; d. a surfactant comprising polyoxyethylene ether alcohol and urea; and e. said nitrogen-containing polyoxyalkylene nonionic surfactant; the method comprising the steps of: i. dissolving said reagents together in water to produce a liquid reagent; ii. separately incubating a biological specimen to be tested and said liquid reagent at a predetermined temperature for a predetermined period of time; iii. mixing said liquid reagent with said specimen to form a specimen/reagent test system; iv. maintaining said system at a predetermined elevated temperature; and v. measuring the change in absorbance of said system by measuring the absorbance at two different predetermined points in time following the formation of said system; vi. determining said lactate dehydrogenase from said change in absorbance.
Claim 4
Dependent Claim
30 words
A reagent formulation as set forth in claim 1 wherein said nitrogen-containing surfactant is solid and the polyoxypropylene chains thereof have an average molecular weight of less than about 4,000.
Claim 5
Dependent Claim
103 words
A substrate reagent formulation for use in assaying a biological specimen for lactate dehydrogenase comprising a solid, water-soluble, substantially anhydrous, storage-stable mixture containing: a. lithium lactate; b. tris-(hydroxymethyl)-aminomethane; c. sodium bicarbonate; d. a surfactant comprising polyoxyethylene ether alcohol and urea; and e. a nitrogen-containing polyoxylalkylene nonionic surfactant obtained by the sequential reaction of ethylenediamine first with propylene oxide and then with ethylene oxide in the presence of a catalyst, the polyoxypropylene chains of said surfactant having an average molecular weight of between about 750 and about 6,750 and the polyoxyethylene chains constituting between about 10 to about 80 weight percent of said surfactant.
Claim 6
Dependent Claim
30 words
A reagent formulation as set forth in claim 3 wherein said nitrogen-containing surfactant is solid and the polyoxypropylene chains thereof have an average moleCular weight of less than about 4,000.
Claim 7
Independent Claim
23 words
The method of assaying a biological specimen for lactate dehydrogenase using a plurality of solid, water-soluble, substantially anhydrous, storage-stable reagent formulations respectively comprising:
Claim 8
Dependent Claim
32 words
The method as set forth in claim 5 wherein said nitrogen-containing polyoxyalkylene non-ionic surfactant is solid and the polyoxypropylene chains thereof have an average molecular weight of not more than about 4,000.
Claim 9
Independent Claim
30 words
The method of preparing a solid, water-soluble, free-flowing, substantially anhydrous, storage-stable substrate reagent formulation for use in assaying a biological specimen for lactate dehydrogenase, said method comprising the steps of:
Claim 10
Independent Claim
30 words
The method of preparing a solid, water-soluble, free-flowing, substantially anhydrous, storage-stable coenzyme reagent formulation for use in assaying a biological specimen for lactate dehydrogenase, said method comprising the steps of:
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-06-10 | 1977-12-10 | 1978-06-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-06-10 | 1981-12-10 | 1982-06-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-06-10 | 1985-12-10 | 1986-06-11 | $7700.00 | Window Closed / Lapsed |