US3816254A
Expired / Lapsed
US
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Optical resolution of racemic amino acids
Assignee: Tanabe Pharma Corp
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Filing: 1972-12-14
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Granted: 1974-06-11
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Expires: 1992-12-14
Abstract
Abstract Mold acylase is treated with a substantially water insoluble haloacetyl-polysaccharide. The resultant substantially waterinsoluble mold acylase preparation is incubated with a solution of an alpha-N-acyl derivation of a racemic acid. Free L-amino acid forms.
Inventors
- 👤 I Chibata
- 👤 T Tosa
- 👤 T Sato
- 👤 T Mori
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 22:48
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12N; C12N11/00; C12N11/02; C12N11/10; C12P41/00; C12P41/006; C12P41/007
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
15 words
The method according to claim 1 characterized in that said agent is an inorganic sulfate.
Claim 2
Dependent Claim
17 words
The method according to claim 1 characterized in that said agent is ammonium sulfate or sodium sulfate.
Claim 3
Dependent Claim
26 words
The method according to claim 1 characterized in that said agent is employed in an amount of 1.0 to 3.2 millimoles per ml of reaction mixture.
Claim 4
Independent Claim
91 words
A method of resolving a racemic amino acid into an optically active component which comprises contacting mold acylase with a substantially water-insoluble haloacetyl-polysaccharide in the presence of 0.6 to 3.6 millimoles per ml of reaction mixture of an agent which salts out protein, to produce a substantially water-insoluble mold acylase preparation, continuously flowing an aqueous solution of an alpha-N-acyl derivative of the racemic amino acid through a column of said preparation to produce an effluent containing free L-amino acid and alpha-N-acyl-D-amino acid, and separating the L-amino acid from the alpha-N-acyl-D-amino acid.
Claim 5
Dependent Claim
28 words
The method according to claim 1 characterized in that said haloacetyl-polysaccharide is selected from the group consisting of bromoacetyl-cellulose, bromoacetyl-dextran, bromoacetyl-agarose, chloroacetyl-cellulose, chloroacetyl-dextran, chloroacetyl-agarose, iodoacetyl-cellulose, iodoacetyl-dextran and iodoacetyl-agarose.
Claim 6
Dependent Claim
33 words
The method according to claim 1 characterized in that said mold acylase is selected from the group consisting of acylases produced by Aspergillus oryzae, Penicillium vinaceum, Aspergillus meleus, Aspergillus midulans and Penicillium corymbiferum.
Claim 7
Dependent Claim
21 words
The method according to claim 1 characterized in that said haloacetyl-polysaccharide contains about 4 to 40 w/w percent of halogen atom.
Claim 8
Dependent Claim
29 words
The method according to claim 8 characterized in that said haloacetyl-poLysaccharide contains about 8 w/w percent chlorine or about 18 w/w percent bromine or about 31 w/w percent iodine.
Claim 9
Dependent Claim
24 words
The method according to claim 8 characterized in that 0.1 to 0.25 g of said mold acylase are present per gram of said haloacetyl-polysaccharide.
Claim 10
Dependent Claim
36 words
The method according to claim 1 characterized in that said solution of the alpha-N-acyl derivative of the racemic amino acid is an aqueous solution and the concentration of the racemate therein is 0.2 - 1.0 mol/liter.
Claim 11
Dependent Claim
60 words
A substantially water-insoluble mold acylase preparation comprising mold acylase covalently bonded to a substantially water-insoluble haloacetyl-polysaccharide, said water-insoluble acylase being prepared by contacting mold acylase with a substantially water-insoluble haloacetyl-polysaccharide, in the presence of an agent which salts out protein, said agent being employed in an amount of 0.6 to 3.6 millimols per ml., thereby producing said water-insoluble mold acylase.
Claim 12
Dependent Claim
27 words
The preparation as claimed in claim 12, wherein said haloacetyl-polysaccharide is selected from the group consisting of bromoacetyl-cellulose, bromoacetyl-dextran, bromoacetyl-agarose, chloroacetyl-cellulose, chloroacetyl-dextran, chloroacetyl-agarose, iodoacetyl-cellulose, iodoacetyl-dextran and iodoacetyl-agarose.
Claim 13
Dependent Claim
32 words
The preparation as claimed in claim 12, wherein said mold acylase is selected from the group consisting of acylases produced by Aspergillus oryzae, Penicillium vinaceum, Aspergillus meleus, Aspergillus midulans and Penicillium corymbiferum.
Claim 14
Dependent Claim
20 words
The preparation as claimed in claim 12, wherein said haloacetyl-polysaccharide contains about 4 to 40 w/w percent of halogen atom.
Claim 15
Dependent Claim
27 words
The preparation as claimed in claim 15, wherein said haloacetyl-polysaccharide contains about 8 w/w percent chlorine, about 18 w/w percent bromine or about 31 w/w percent iodine.
Claim 16
Dependent Claim
23 words
The preparation as claimed in claim 12, wherein 0.1 to 0.25 g of said mold acylase are bonded per gram of said haloacetyl-polysaccharide.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-06-10 | 1977-12-10 | 1978-06-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-06-10 | 1981-12-10 | 1982-06-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-06-10 | 1985-12-10 | 1986-06-11 | $7700.00 | Window Closed / Lapsed |