US3816249A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Universal medium and method for extending the useful life of semen in vitro
Assignee: Applied Generics Ltd
•
Filing: 1973-02-28
•
Granted: 1974-06-11
•
Expires: 1993-02-28
Abstract
Abstract A universal medium for extending the useful life of semen, in vitro, is provided comprising, in effective amounts, egg yolk, Alpha -aminopropionic acid, and glycine. If the semen is to be stored for long periods of time at reduced temperature it is preferred that glycerol and an ionizable salt, such as sodium chloride, be utilized in the medium. The medium has general utility for extending the life of semen from all types of mammals and birds and is especially valuable for extending the useful life of sensitive types of semen, such as turkey semen. Such sensitive types of semen can now be kept viable until they can be frozen. The invention provides for storage by freezing of types of semen which have not before been successfully frozen.
Inventors
- 👤 B Bhattacharya
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 16:06
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/52
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
45 words
The method of claim 1 wherein the semen is collected from a plurality of male birds directly into said aqueous medium in a container and the mixture of semen and medium after storage in said container is used to inseminate a plurality of female birds.
Claim 2
Dependent Claim
21 words
The method of claim 1 wherein from about 4 to 12 percent by weight of glycerol is included in the medium.
Claim 3
Dependent Claim
26 words
The method of claim 2 wherein the semen is collected from a plurality of turkey toms and is used to inseminate a flock of turkey hens.
Claim 4
Dependent Claim
22 words
The method of claim 1 wherein the aqueous medium additionally contains a minor but effective amount of neomycin to control bacterial infections.
Claim 5
Dependent Claim
54 words
The method of claim 2 wherein from about 4 to 12 percent by weight of glycerol is included in the medium and the container of semen and medium is stored at a temperature of about 4* to 10*C for a time period of up to 48 hours prior to insemination of the female birds.
Claim 6
Dependent Claim
32 words
The method of claim 6 wherein the mixture of semen and medium after reaching equilibrium at 4* to 10*C is frozen in the container and is stored in frozen form until use.
Claim 7
Independent Claim
35 words
A mediUm for extending the useful life of semen, in vitro, comprising a mixture of glycine, Alpha -aminopropionic acid and egg yolk in amounts effective in aqueous solution to extend the life of said semen.
Claim 8
Dependent Claim
34 words
The medium of claim 8 containing an ionizable chloride salt selected from the group consisting of sodium chloride, potassium chloride and calcium chloride in an amount effective to facilitate the storage of said semen.
Claim 9
Dependent Claim
18 words
The medium of claim 9 wherein the ingredients are in the form of a dry powdered solid admixture.
Claim 10
Dependent Claim
23 words
The medium of claim 9 wherein the ingredients are in aqueous solution at a pH in the range of about 6.0 to 8.0.
Claim 11
Dependent Claim
22 words
The medium of claim 11 wherein glycerol is present in an amount effective to facilitate storage of the solution at low temperatures.
Claim 12
Dependent Claim
12 words
The medium of claim 11 in admixture with semen containing live spermatozoa.
Claim 13
Dependent Claim
97 words
A universal medium for extending the useful life of semen, in vitro, comprising an aqueous solution having a pH in the range of from about 6.0 to 8.0, said solution containing, by weight, from about 0.01 percent to about 1.0 percent glycine, from about 0.01 percent to about 1.0 percent Alpha -aminopropionic acid, from about 0.1 percent to 2.0 percent of sodium chloride, potassium chloride or calcium chloride, from about 4 percent to about 12 percent of glycerol, from about 30 to about 55 percent egg yolk, and from about 30 percent to about 70 percent water.
Claim 14
Dependent Claim
80 words
A universal medium for extending the useful life of semen, in vitro, comprising a clear solution having a pH in the range of from about 6.0 to 8.0 and containing from about 0.01 to 1.0 percent glycine, from about 0.01 to 1.0 percent Alpha -aminopropionic acid, from about 0.1 to 2.0 percent sodium chloride, from about 30 to 70 percent water, and from about 30 to 55 percent of the components of egg yolk which are soluble in the mixture.
Claim 15
Dependent Claim
11 words
The medium of claim 15 admixed with semen containing live spermatozoa.
Claim 16
Dependent Claim
8 words
The composition of claim 16 in encapsulated form.
Claim 17
Dependent Claim
22 words
The medium of claim 15 wherein the pH of the solution is about 6.2 and the osmolality is from about 296-315 mos/kg.
Claim 18
Dependent Claim
16 words
The medium of claim 15 wherein from about 4 to 12 percent of glycerol is included.
Claim 19
Independent Claim
105 words
A method for preparing a composition for extending the useful life of semen comprising mixing egg yolk, glycine, Alpha -aminopropionic acid, sodium chlrode and water to provide a solution having a pH in the range of about 6.0 to 8.0 and containing, by weight, from about 30 to 55 percent egg yolk, from about 0.01 to 1.0 percent glycine, from about 0.01 to 1.0 percent Alpha -aminopropionic acid, from about 0.01 to 2.0 percent of sodium chloride and from about 30 to 70 percent water, permitting the mixture to stand to form a precipitate, and separating the precipitate to provide a substantially clear product mixture.
Claim 20
Dependent Claim
17 words
The method of claim 20 wherein the clear solution is dried and a solid product is obtained.
Claim 21
Dependent Claim
18 words
The method of claim 20 wherein about 4 to 12 percent of glycerol is added to the mixture.
References Cited (Backward Citations)
US3185623A
Cited
US3431172A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-06-10 | 1977-12-10 | 1978-06-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-06-10 | 1981-12-10 | 1982-06-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-06-10 | 1985-12-10 | 1986-06-11 | $7700.00 | Window Closed / Lapsed |