US3785735A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Photoanalysis method
Assignee: BIO PHYSICS SYSTEMS Inc
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Filing: 1972-01-19
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Granted: 1974-01-15
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Expires: 1992-01-19
Abstract
Abstract A narrow beam of light is directed through an optical chamber to intersect a thin stream of small particles to be optically analyzed. At least two photoresponsive pick-up elements are positioned at different angular positions with respect to the beam to simultaneously detect different optical reactions of each particle to the beam illumination.
Inventors
- 👤 M Friedman
- 👤 I Klinger
- 👤 L Kamentsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 23:22
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1434; G01N15/1456; G01N15/1459; G01N21/00; G01N21/17; G01N21/47; G01N21/49; G01N21/53; G01N15/1404; G01N2015/1413; G01N2021/4704; G01N2021/4707; G01N2021/4709; G01N2021/4711
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
224 words
A method for rapidly detecting biological cells having different properties including the steps of exposing a liquid solution containing the cells to be analyzed to a dye which is taken up by cells having one characteristic and not taken up to a significant extent by cells having other characteristics, moving the liquid solution in a thin stream to convey the cells one by one through a housing comprised of a material which transmits light and defining an optical chamber while directing light from a light source into one side of the housing to intersect with the thin stream of cells in a narrow incident beam, the narrow incident beam converging at the stream of cells and intercepting the entire width of the particle stream, and detecting optical signals by means of photoresponsive pickup elements positioned outside of the housing at different angular positions with respect to the direction of the incident beam when measured from the intersection of the incident beam with the stream of cells, at least one of the photoresponsive pick-up elements being effective to detect optical absorption of said incident beam to indicate cells which took up the dye, and at least one other photoresponsive pick-up element being effective to detect scatter of said incident beam by the cells to thereby detect the cells which did not take up the dye.
Claim 2
Independent Claim
224 words
A method for rapidly detecting biological cells having different properties including the steps of exposing a liquid solution containing the cells to be analyzed to a dye which is taken up by cells having one characteristic and not taken up to a significant extent by cells having other characteristics, moving the liquid solution in a thin stream to convey the cells one by one through a housing comprised of a material which transmits light and defining an optical chamber while directing light from a light source into one side of the housing to intersect with the thin stream of cells in a narrow incident beam, the narrow incident beam converging at the stream of cells and intercepting the entire width of the particle stream, and detecting optical signals by means of photoresponsive pickup elements positioned outside of the housing at different angular positions with respect to the direction of the incident beam when measured from the intersection of the incident beam with the stream of cells, at least one of the photoresponsive pick-up elements being effective to detect optical absorption of said incident beam to indicate cells which took up the dye, and at least one other photoresponsive pick-up element being effective to detect scatter of said incident beam by the cells to thereby detect the cells which did not take up the dye.
Claim 3
Dependent Claim
45 words
A method as claimed in claim 1 wherein said photoresponsive pick-up element which is effective to detect absorption is positioned at an angular position out of alignment with the direction of the incident beam so as to measure absorption in terms of a scatter signal.
Claim 4
Dependent Claim
53 words
A method as claimed in claim 1 wherein the photoresponsive pick-up element effective to detect absorption is positioned at essentially a zero angular position with respect to the direction of the incident beam so as to be in line with the incident beam for the direct measurement of optical absorption by the cells.
Claim 5
Dependent Claim
54 words
A method as claimed in claim 1 wherein the liquid solution containing the cells is moved in a thin stream to convey the cells one by one through the housing by causing the stream of cells to be narrowed down at the entrance of the housing as the solution is moved through the housing.
Claim 6
Dependent Claim
66 words
A method as claimed, in claim 4 wherein the liquid solution is narrowed down to a thin stream by conveying a second compatible liquid through the housing as an annular sheath of liquid surrounding the liquid solution and causing the liquid solution to be confined to a thin stream at the center of the sheath of surrounding compatible liquid as both are moved through the housing.
Claim 7
Dependent Claim
36 words
A method as claimed in claim 5 wherein the liquid solution is narrowed down to a thin stream having a maximum dimension of the same order of magnitude as the maximum dimension of the individual cells.
Claim 8
Dependent Claim
57 words
A method as claimed in claim 6 wherein there is included the step of diluting the solution containing the cells so that the maximum concentration of cells is in the order of one million cells per milliliter of solution to Thereby assure that the cells pass the point of intersection with the light beam one by one.
Claim 9
Dependent Claim
48 words
A method as claimed in claim 1 wherein the cells to be analyzed are blood cells and the characteristic to be determined is whether or not the cells are alive, the dye being taken up by the dead cells and not significantly taken up by the live cells.
Claim 10
Dependent Claim
13 words
A method as claimed in claim 8 wherein the dye is trypan blue.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-01-14 | 1977-07-16 | 1978-01-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-01-14 | 1981-07-16 | 1982-01-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-01-14 | 1985-07-16 | 1986-01-15 | $7700.00 | Window Closed / Lapsed |