Abstract An aqueous culture medium comprising (1) 3 to 35 grams per liter of alkali metal, calcium nitrate, magnesium nitrate, strontium nitrate and/or beryllium nitrate; (2) 0.3 to 10 grams per liter of alkali metal phosphate; (3) 1 to 15 grams per liter of an alkali metal acetate, calcium acetate, magnesium acetate, strontium acetate and/or beryllium acetate; and (4) a nutrient source; and a process for the production of an alpha,omegaalkanedioic acid of 10 to 16 carbon atoms comprising (1) introducing a mutated strain of Corynebacterium into a composition which has a pH of from 6 to 9 and which comprises 1 to 50 percent by volume of an n-alkane of 10 to 16 carbon atoms and a complemental amount of the culture medium above; (2) maintaining the composition at a temperature between 20* and 45*C. for 48 t0 96 hours; and (3) separating the alpha,omegaalkanedioic acid from the resulting mixture.
The process of claim 1 wherein the n-alkane is from 10 to 14 carbon atoms, the amount of n-alkane in the composition is 5 to 20 percent by volume, and the amount of culture medium is 80 to 95 percent by volume.
Claim 2
Dependent Claim
171 words
A process for the production of an alpha, omega-alkanedioic acid of 10 to 16 carbon atoms which comprises (1) introducing a mutated strain of Corynebacterium capable of producing at high yield and conversion alpha, omega-alkanedioic acid of 10 to 16 carbon atoms into a composition which has a pH of from 6 to 9 and which comprises 1 to 50 percent by volume of n-alkane of 10 to 16 carbon atoms and from 50 to 99 percent by volume of a culture medium comprising (a) 6 to 30 grams per liter of a member of the group consisting of sodium nitrate and potassium nitrate, (b) 1 to 6 grams per liter of an alkali metal phosphate, (c) 2.5 to 10 grams per liter of a member selected from the group consisting of sodium acetate and potassium acetate and (d) a nutrient source; (2) maintaining the composition at a temperature of between about 20*C. and 45*C. for 48 to 96 hours and (3) separating the alpha, omega-alkanedioic acid from the resulting mixture.
Claim 3
Dependent Claim
43 words
The process of claim 3 wherein the n-alkane is from 10 to 14 carbon atoms, the volume of the n-alkane in the composition is 5 to 20 percent by volume, and the amount of culture medium is 80 to 95 perCent by volume.
Claim 4
Dependent Claim
12 words
The process of claim 4 wherein the n-alkane contains 12 carbon atoms.