Apparatus for assaying enzyme activity
Abstract
Abstract IMPROVED METHOD AND APPARATUS FOR QUANTITATING THE ACTIVITIES OF A WIDE SELECTION OF ENZYMES PRESENT IN BIO: LOGICAL FLUIDS, USING A SPOT TEST TECHNIQUE. THE TEST ULTILIZES ENZYME STANDARDS FREEZE-DRYED ON TRANSPARENT MEMBRANES ASSEMBLED TOGETHER WITH BUT SEPARATED FROM REAGENTS, INCLUDING SUBSTRATES, DYES, AND COFACTORS, WHICH ARE FREEZEDRYED IN ABSORBENT PADS. THE ASSEMBLY MAY UTILIZED MICROPOROUS MEMBRANES AS FILTERS TO ASSIST IN PROCESSING WHOLE BLOOD SAMPLES. AS ASSEMBLED, THE SPOT TEST PLATES OR SLIDES ARE COMPLETELY SELT-SUFFIIENT, REQUIRE NO ADDED CONTROLS, INSTRUMENTATION, OR LIQUID VOLUME MEASUREMENT. THE TESTS MAY BE UTILIZED UNDER ANY ENVIRONMENTAL CONDITIONS SUCH AS THOSE REQUIRING RAPID DIAGNOSTIC SUPPORT UNDER EMERGENCY CONDITONS, OR ROUTINELY BY MEDICAL PRACTITIONERS IN OFFICES, HOSPITIALS OR CLINICS. TEST PLATES MAY ASSIST IN THE DIAGNOSIS OF A WIDE SPECTRUM OF PATHOLOGICAL CONDITIONS RELATING TO MALFUNCTIONS OF THE LIVER, SPLEEN, HEART, NEURALOGICAL SYSTEM,, LUNGS, KIDNEYS, MUSCLE TISSUE, BONES, ETC.
Inventors
- 👤 R Moyer
- 👤 D Sibbett
Bibliographic Details
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