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US3779866A Expired / Lapsed US ⏱️ 0.0 years remaining

Microbial degradation of polychlorinated biphenyls

Assignee: BIOTEKNIKE INT Inc
•
Filing: 1972-04-07
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Granted: 1973-12-18
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Expires: 1992-04-07
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Abstract

Abstract A process for the microbial degradation of polychlorinated biphenyls (PCBs) which comprises treating the PCBs with certain non-pathogenic, hydrocarbon-utilizing strains of Cladosporium cladosporioides, Candida lipolytica, Nocardia globerula, Nocardia rubra and/or Saccharomyces cerevisiae until the PCBs have been substantially degraded. The process is applicable degrading PCBs as they may be present as pollutants or contaminants in water, in industrial effluents, in various land areas such as industrial sites and the like or in varied laboratory or commercial installations. The process may also be used to clean up and degrade mixtures of PCBs and various hydrocarbon oils or petrochemicals whenever their presence constitutes a deleterious pollution.

Inventors

  • 👤 E Azarowicz

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-27 13:36
CPC Classifications
C; C02; C02F; C02F3/00; C02F3/34; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/872; Y10S435/911; Y10S435/921; Y10S435/923; Y10S435/94; Y10S435/942

Claims Analysis

Total Claims: 28
Claim 1
Independent Claim 14 words
The process of claim 1, wherein said microorganism is mixed with a cellulosic material.
Claim 2
Dependent Claim 21 words
The process of claim 1, wherein said microorganism is mixed with a cellulosic material, a nitrogen source and a phosphorus source.
Claim 3
Dependent Claim 33 words
The process of claim 1, wherein said microorganism is added to an aqueous solution containing the polychlorinated biphenyls in slurry form in an amount of approximately 1 percent to 5 percent by volume.
Claim 4
Dependent Claim 32 words
The process of claim 2, wherein said cellulosic material is selected from the group consisting of sawdust, pine bark, wood flour, cotton linters, cottonseed hulls, straw, bagasse, marsh hay and shredded paper.
Claim 5
Dependent Claim 12 words
The process of claim 3, wherein said nitrogen source is cottonseed protein.
Claim 6
Dependent Claim 14 words
The process of claim 1, wherein said microorganism is employed in a slurry form.
Claim 7
Dependent Claim 14 words
The process of claim 1, wherein said microorganism is employed in a pelletized form.
Claim 8
Dependent Claim 14 words
The process of claim 1, wherein said microorganism is employed in a powdered form.
Claim 9
Dependent Claim 16 words
The process of claim 1, wherein said microorganism is employed in the form of a foam.
Claim 10
Dependent Claim 59 words
A process for the microbial degradation of polychlorinated biphenyls which comprises treating said polychlorinated biphenyls with at least one microorganism selected from the group consisting of Cladosporium cladosporioides ATCC 20251, Candida lipolytica ATCC 20255, Nocardia globerula ATCC 21505, Nocardia rubra ATCC 21508, and Saccharomyces cerevisiae ATCC 20252 for a sufficient time until the polychlorinated biphenyls have been substantially degraded.
Claim 11
Dependent Claim 14 words
The process of claim 11, wherein said microorganism is mixed with a cellulosic material.
Claim 12
Dependent Claim 21 words
The process of claim 11, wherein said microorganism is mixed with a cellulosic material, a nitrogen source and a phosphorus source.
Claim 13
Dependent Claim 36 words
The process of claim 11, wherein the polychlorinated biphenyls are contained in an aqueous solution and said microorganism is added thereto in slurry form in an amount of about 1 percent to 5 percent by volume.
Claim 14
Dependent Claim 23 words
The process of claim 14, wherein about 2 percent by volume of the microorganism slurry is added to the solution of polychlroinated biphenyls.
Claim 15
Dependent Claim 25 words
The process of claim 14, wherein the concentration of polychlorinated biphenyls in said aqueous solution is about 10 p.p.m. to about 15 percent by weight.
Claim 16
Dependent Claim 32 words
The process of claim 12, wherein said cellulosic material is selected from the group consisting of sawdust, pine bark, wood flour, cotton linters, cottonseed hulls, straw, bagasse, marsh hay and shredded paper.
Claim 17
Dependent Claim 12 words
The process of claim 13, wherein said nitrogen source is cottonseed protein.
Claim 18
Dependent Claim 14 words
The process of claim 11, wherein said microorganism is employed in a slurry form.
Claim 19
Dependent Claim 14 words
The proCess of claim 11, wherein said microorganism is employed in a pelletized form.
Claim 20
Dependent Claim 14 words
The process of claim 11, wherein said microorganism is employed in a powdered form.
Claim 21
Dependent Claim 16 words
The process of claim 11, wherein said microorganism is employed in the form of a foam.
Claim 22
Dependent Claim 23 words
The process of claim 11, wherein said microorganism is added to the polychlorinated biphenyls at a temperature of approximately 4*C. to approximately 39*C..
Claim 23
Dependent Claim 13 words
The process of claim 11, wherein said microorganism is Cladosporium cladosporioides ATCC 20251.
Claim 24
Dependent Claim 13 words
The process of claim 11, wherein said microoganism is Candida lipolytica ATCC 20255.
Claim 25
Dependent Claim 13 words
The process of claim 11, wherein said microorganism is Nocardia globerula ATCC 21505.
Claim 26
Dependent Claim 13 words
The process of claim 11, wherein said microorganism is Nocardia rubra ATCC 21508.
Claim 27
Dependent Claim 13 words
The process of claim 11, wherein said microorganism is Saccharomyces cerevisiae ATCC 20252.
Claim 28
Dependent Claim 23 words
The process of claim 11, wherein the polychlorinated biphenyls are contained in an aqueous solution which additionally contains hydrocarbon oils or other petrochemicals.

References Cited (Backward Citations)

US3616204A Cited
US3634227A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1976-12-17 1977-06-18 1977-12-18 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1980-12-17 1981-06-18 1981-12-18 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1984-12-17 1985-06-18 1985-12-18 $7700.00 Window Closed / Lapsed

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