US3773645A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrophoresis device
Assignee: Colora Messtechnik GmbH
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Filing: 1971-11-12
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Granted: 1973-11-20
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Expires: 1991-11-12
Abstract
Abstract An electrophoresis device having a separating chamber, and two buffer containers one of which is attached to one end of said separating chamber and the other one is connected to the other end, and an electrode in each in said buffer containers, said device essentially having a box-shaped housing and a box area which is divided up into two partial areas serving as buffer containers by means of an essentially horizontal intermediate bottom of lesser depth than the box together with an intermediate wall which extends essentially vertically from the intermediate bottom.
Inventors
- 👤 S Nees
- 👤 W Schmidt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 14:09
CPC Classifications
B; B01; B01D; B01D57/00; B01D57/02; G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44756
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
28 words
A device as claimed in claim 1 wherein said elution means further comprises a second elongated chamber beneath said elongated elution chamber for receiving a concentrated buffer fluid.
Claim 2
Dependent Claim
22 words
A device as claimed in claim 1 wherein the lower wall of said elongated elution chamber is composed of semipermeable membrane material.
Claim 3
Dependent Claim
49 words
A device as claimed in claim 2 wherein said second elongated chamber has an upper and a lower wall each composed of semipermeable membrane material, said lower wall separating said second chamber from said other buffer container and said upper wall constituting the lower wall of said elution chamber.
Claim 4
Dependent Claim
30 words
A device as claimed in claim 2 and further comprising means at each of the opposed ends of said second elongated chamber for supplying and removing the concentrated buffer fluid.
Claim 5
Dependent Claim
60 words
A device as claimed in claim 1 wherein said elution means comprises a plate having a recess therein which constitutes said elongated elution chamber, the shape of said recess conforming with the shape of the lower end of said separating chamber and means for tightly securing the upper side of said plate to the lower end of said separating chamber.
Claim 6
Dependent Claim
26 words
A device as claimed in claim 6 wherein said recess extends through said plate and further comprising means for closing the lower side of said recess.
Claim 7
Dependent Claim
54 words
A device as claimed in claim 6 wherein said elution means further includes a second plate beneath said first mentioned plate and having a recess therein for receiving a concentrated buffer fluid and a semipermeable membrane firmly gripped between said first and second plates and constituting the lower wall of said elongated elution chamber.
Claim 8
Dependent Claim
25 words
A device as claimed in claim 8 wherein the recess of said second mentioned plate corresponds in shape to the recess of said first plate.
Claim 9
Dependent Claim
42 words
A device as claimed in claim 8 wherein said elution means further includes a third plate and a second semipermeable membrane gripped between said third plate and said second plate to form the lower wall of the recess in said second plate.
Claim 10
Dependent Claim
24 words
A device as claimed in claim 10 wherein the recess of said third plate corresponds in shape to the recess in said first plate.
Claim 11
Dependent Claim
32 words
A device as claimed in claim 6 wherein the means for supplying and removing an elution fluid comprises channels leading to and from the opposed ends of the recess in said plate.
Claim 12
Dependent Claim
37 words
A device as claimed in claim 8 and further comprising channel means in said second plate for supplying and removing the concentrated buffer fluid to and from the opposed ends of the recess in said second plate.
Claim 13
Dependent Claim
62 words
A device as claimed in claim 10 wherein said first, second and third plates are superposed with semipermeable membranes disposed therebetween, said recesses being in register and conforming with the shape of the lower end of said separating chamber and further comprising means for pressing the superposed plates together and for pressing the assembled plates against the bottom of said separating chamber.
Claim 14
Dependent Claim
38 words
A device as claimed in claim 1 and further comprising baffle rods disposed within said elongated elution chamber, arranged alternately on opposite sides thereof and extending diagonally to the direction of flow of the elution fluid passing therethrough.
Claim 15
Dependent Claim
36 words
A device as claimed in claim 1 and further comprising vertical walls defining said separating chamber, said walls being provided with channels for supplying and removing the elution fluid to and from said elongated elution chamber.
Claim 16
Dependent Claim
33 words
A device as claimed in claim 1 and further comprising adjustable means within the other buffer container for supporting said elution means and pressing it against the lower end of said separating chamber.
Claim 17
Dependent Claim
90 words
A gel electrophoresis device comprising two laterally spaced parallel, vertical walls forming a vertical separating chamber therebetween, two buffer containers on opposed sides of said separating chamber, one being only in communication with the upper end of said separating chamber and the other only in communication with the lower end of said separating chamber, whereby each container may be easily filled with buffer fluids to the same level in each container, an electrode in each of said containers and means for connecting said electrodes to a source of direct current.
Claim 18
Independent Claim
23 words
A device as claimed in cliam 18 wherein each of the laterally spaced walls form one wall of each of the buffer containers.
Claim 19
Dependent Claim
26 words
A device as claimed in claim 18, wherein one of said laterally spaced walls extends upwardly to a higher point than the other of said walls.
Claim 20
Dependent Claim
45 words
A device as claimed in claim 18 and further comprising a lid member disposed above said separating chamber, and wherein said laterally spaced walls extend upwardly to the same level, said lid member forming a fluid tight seal with one of said laterally spaced walls.
Claim 21
Dependent Claim
120 words
A device as claimed in claim 18 and further comprising a substantially rectangular box-like housing having a bottom and side elements and including an intermediate bottom element above the bottom of said housing and extending partially but not less than one half of the distance across said housing, the bottom of one of said laterally spaced walls being connected to the inner end of said intermediate bottom, whereby the intermediate bottom element and said one laterally spaced wall together with side portions of said housing form one of said buffer containers, the other of said buffer containers being defined by the other of said laterally spaced walls, the bottom and side elements of said housing, and the intermediate bottom element.
Claim 22
Dependent Claim
17 words
A device as claimed in claim 22 wherein the space between said laterally spaced walls is adjustable.
Claim 23
Dependent Claim
25 words
A device as claimed in claim 22 wherein a portion of said other buffer container is disposed beneath the lower end of said separating chamber.
Claim 24
Dependent Claim
32 words
A device as claimed in claim 22 and further comprising a detachable lid for said housing and wherein the side element of said housing spaced from said intermediate bottom element is detachable.
Claim 25
Dependent Claim
79 words
A device as claimed in claim 22 wherein the one laterally spaced wall forming a part of said one buffer container is provided with a window therethrough, and further comprising a cover plate for covering said window on that side facing said separating chamber and means for securing said cover plate over said window in said one spaced wall, said means also securing said other spaced wall in spaced relation to said cover plate to form said separating chamber.
Claim 26
Dependent Claim
22 words
A device as claimed in claim 26 and further comprising packing means between said cover plate and said other laterally spaced wall.
Claim 27
Dependent Claim
54 words
A device as claimed in claim 27 wherein said packing means comprises at least two groups of vertically extending parallel members, said groups being spaced apart to divide said separating chamber into at least two parallel, vertical chambers, said packing means being composed of the same material as the material facing said separating chamber.
Claim 28
Dependent Claim
46 words
A device as claimed in claim 26 and further comprising a lid member disposed above said separating chamber, said lid member forming a fluid tight seal with the top of said other-spaced wall to prevent communication between said other buffer container and said one buffer container.
Claim 29
Dependent Claim
43 words
A device as claimed in claim 26 wherein said securing means comprises bolts extending outwardly from said one laterally spaced wall and through said cover plate and said other laterally spaced wall and nuts for securing the cover plate and spaced walls together.
Claim 30
Dependent Claim
31 words
A device as claimed in claim 22 and further comprising a recess on the inside of one wall of each of said two buffer containers for receiving each of said electrodes.
Claim 31
Dependent Claim
36 words
A device as claimed in claim 18 wherein a wall of the other buffer container in communication with the lower end of said separation chamber is provided with a recess for receiving one of said electrodes.
Claim 32
Dependent Claim
37 words
A device as claimed in claim 32 wherein the wall of the one buffer container in communication with the upper end of said separation chamber is provided with a recess for receiving the other of said electrodes.
Claim 33
Dependent Claim
52 words
A device as claimed in claim 31 wherein each of said recesses in the walls is provided with an upwardly inclined upper surface and said walls are provided with channels extending from the uppermost part of said recesses to the top of said walls for removing gas bubbles formed at said electrodes.
Claim 34
Independent Claim
29 words
A device as claimed in claim and further comprising detachable cooling means disposed in each of said buffer containers adjacent said laterally spaced walls for cooling said separation chamber.
Claim 35
Dependent Claim
21 words
A device as claimed in claim 35 wherein said cooling means comprises cooling plates arranged parallel to said laterally spaced walls.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-11-19 | 1977-05-21 | 1977-11-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-11-19 | 1981-05-21 | 1981-11-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-11-19 | 1985-05-21 | 1985-11-20 | $7700.00 | Window Closed / Lapsed |