US3769165A
Expired / Lapsed
US
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Process for producing adenosine triphosphate and adenosine diphosphate
Assignee: KH Neochem Co Ltd
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Filing: 1968-06-14
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Granted: 1973-10-30
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Expires: 1988-06-14
Abstract
Abstract A process for producing adenosine triphosphate (ATP) and adenosine diphosphate (ADP) which comprises culturing a microorganism belonging to the genus Corynebacterium, Micrococcus or Arthrobacter in an aqueous nutrient medium containing adenine, a derivative of adenine, such as adenosine or adenylic acid, or a substance containing the same, accumulating ATP and ADP therein and recovering the products. The yield may be increased by including at least about 0.4 percent by weight of PO4 as inorganic phosphate in the medium.
Inventors
- 👤 K Nakayama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 01:35
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/32; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/83; Y10S435/843; Y10S435/859
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
21 words
The process of claim 1, wherein said derivatives of adenine are selected from the group consisting of adenosine and adenylic acid.
Claim 2
Dependent Claim
19 words
The process of claim 1, wherein said additive is present in the nutrient medium at the initiation of culturing.
Claim 3
Dependent Claim
19 words
The process of claim 1, wherein said additive is added to the nutrient medium after the initiation of culturing.
Claim 4
Dependent Claim
28 words
The process of claim 1, wherein culturing is carried out at a temperature of from about 20* to 40*C. and at a pH of about 5 to 9.
Claim 5
Dependent Claim
22 words
The process of claim 1, wherein the nutrient medium contains at least about 0.4 percent by weight of PO4 as inorganic phosphate.
Claim 6
Dependent Claim
27 words
The process of claim 1, wherein said additive is added to the medium in an amount to provide from about 1 to 10 mg/ml of adenine therein.
Claim 7
Dependent Claim
23 words
The process of claim 1, wherein the pH is maintained at between 5.5 and 7.5 after said additive is added to said medium.
Claim 8
Dependent Claim
11 words
The process of claim 1, wherein said nutrient medium contains glucose.
Claim 9
Dependent Claim
13 words
The process of claim 1, wherein said micro-organism is Corynebacterium mycetoides ATCC 21134.
Claim 10
Dependent Claim
94 words
A process for producing adenosine triphosphate and adenosine diphosphate which comprises culturing a micro-organism belonging to Corynebacterium mycetoides under aerobic conditions at a temperature of from about 20* to 40*C. and at a pH of about 5 to 9 in an aqueous nutrient medium containing about 0.4 to 1.6 percent by weight of PO4 as inorganic phosphate and an additive selected from the group consisting of adenine, derivatives of adenine and substances containing adenine or a derivative thereof, accumulating adenosine triphosphate and adenosine diphosphate in the resultant culture liquor, and recovering the same therefrom.
Claim 11
Dependent Claim
19 words
The process of claim 11, wherein said additive is added to the nutrient medium after the initiation of culturing.
Claim 12
Dependent Claim
23 words
The process of claim 12, wherein the pH is maintained at between 5.5 and 7.5 after said additive is added to said medium.
Claim 13
Dependent Claim
27 words
The process of claim 11, wherein said additive is added to the medium in an amount to provide from about 1 to 10 mg/ml of adenine therein.
Claim 14
Dependent Claim
13 words
The process of claim 11, wherein said micro-organism is Corynebacterium mycetoides ATCC 21134.
Claim 15
Dependent Claim
21 words
The process of claim 11, wherein said derivatives of adenine are selected from the group consisting of adenosine and adenylic acid.
Claim 16
Dependent Claim
11 words
The process of claim 11, wherein said nutrient medium contains glucose.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-29 | 1977-04-30 | 1977-10-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-29 | 1981-04-30 | 1981-10-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-29 | 1985-04-30 | 1985-10-30 | $7700.00 | Window Closed / Lapsed |