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US3764512A Expired / Lapsed US ⏱️ 0.0 years remaining

Laser scanning electrophoresis instrument and system

Assignee: Singer Co
•
Filing: 1972-05-02
•
Granted: 1973-10-09
•
Expires: 1992-05-02
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Abstract

Abstract An improved instrument and system is provided for determining the electrokinetic, or zeta potential of dispersed particles in an aqueous solution. The system to be described includes a helium-neon laser, electrophoresis cell, a lens system for expanding and focusing the laser beam to produce a focal spot within the cell, a galvanometer driven mirror for reflecting the laser beam from the lens system into the cell, electronic circuitry for applying a potential across the electrodes of the cell causing the dispersed particles to migrate from one electrode to the other electrode, electronic scanning circuitry for the galvanometer driven mirror and a viewing microscope. The operator controls the angular displacement and rate of angular displacement of the mirror until the focused laser spot, as viewed through the microscope, tracks the particles in the cell. A control may be calibrated directly in zeta potential. The determination of the zeta potential is useful in determining the degree of stability of particle dispersion in aqueous solutions, and the like. The function of the scanning instrument and system of the present invention is to measure the zeta potential of colloidal particles dispersed in a solution.

Inventors

  • 👤 I Greenwood
  • 👤 J Kaufman

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-24 13:26
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721

Claims Analysis

Total Claims: 12
Claim 1
Independent Claim 109 words
An instrument for measuring the electrophoretic mobility of particles dispersed in a liquid and under the influence of an electric field, said instrument including: an electrophoresis cell for receiving and holding a liquid sample having electrophoretic responsive particles therein and in which said particles migrate under the infludence of an electric field; means for directing an illuminating beam at said cell to illuminate the particles migrating in the sample; a microscope positioned to enable the particles in the cell as illuminated by the beam to be viewed by an observer; and means for scanning the beam to cause it to track the movement of the particles in the cell.
Claim 2
Dependent Claim 109 words
An instrument for measuring the electrophoretic mobility of particles dispersed in a liquid and under the influence of an electric field, said instrument including: an electrophoresis cell for receiving and holding a liquid sample having electrophoretic responsive particles therein and in which said particles migrate under the infludence of an electric field; means for directing an illuminating beam at said cell to illuminate the particles migrating in the sample; a microscope positioned to enable the particles in the cell as illuminated by the beam to be viewed by an observer; and means for scanning the beam to cause it to track the movement of the particles in the cell.
Claim 3
Dependent Claim 33 words
The instrument defined in claim 1, in which said directing means comprises a laser and a lens system for focusing the beam from the laser into a focal spot within the electrophoresis cell.
Claim 4
Dependent Claim 17 words
The instrument defined in claim 1, in which said scanning means comprises a mirror-galvanometer type beam deflector.
Claim 5
Dependent Claim 26 words
The instrument defined in claim 1, and which includes an adjustable power supply connected to said electrophoresis cell for producing an electric field within the cell.
Claim 6
Dependent Claim 43 words
The instrument defined in claim 3, and which includes electronic circuitry coupled to said galvanometer for producing the scanning action of the light beam, said electronic circuitry including manual controls for controlling the angular displacements of the beam and the rate of displacement.
Claim 7
Dependent Claim 49 words
The instrument defined in claim 2, in which said electrophoresis cell has a rectangular cross section, and in which said lens system focuses the light beam from the laser cell to a focal point in a region of said cell in which the average electro-endosmotic velocity is essentially zero.
Claim 8
Dependent Claim 59 words
The instrument defined in claim 5, and which includes modulating circuitry in circuit with said electronic circuitry for introducing an additional scanning action to the galvanometer so that the light beam is moved to one side of its normal position for short periods and to the other side of its normal position for periods longer than said short periods.
Claim 9
Dependent Claim 57 words
The combination defined in claim 1, in which said scanning means comprises a galvanometer, and which includes electronic circuitry connected through said galvanometer for introducing a scanning signal thereto, and an adjustable power supply connected to said electronic circuitry and to said electrophoresis cell to introduce a common adjustable voltage to said circuitry and to said cell.
Claim 10
Dependent Claim 26 words
The instrument defined in claim 8, and which includes a control in said electronic circuitry for adjusting the scanning rate of the beam in said cell.
Claim 11
Dependent Claim 16 words
The instrument defined in claim 9, in which said last-named control is calibrated in zeta potential.
Claim 12
Dependent Claim 15 words
The instrument defined in claim 9, in which said last-named control is calibrated in mobility.

References Cited (Backward Citations)

US2412602A Cited
US2379158A Cited
US2494441A Cited
US3320148A Cited
US3454487A Cited
US3498905A Cited
US3523733A Cited
US3519353A Cited
US3511227A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1976-10-08 1977-04-09 1977-10-09 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1980-10-08 1981-04-09 1981-10-09 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1984-10-08 1985-04-09 1985-10-09 $7700.00 Window Closed / Lapsed

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