Enzymatic hydrolysis of cellulose to soluble sugars
Abstract
Abstract A METHOD FOR IMMOBILIZATION OF THE ENZYME, CELLULASE, BY ADSORPTION ON ITS INSOLUBLE SUBSTRATE, CELLULOSE, PERMITS SIMPLE METHODS FOR EFFICIENT ENXMATIC HYDROLYSIS OF CELLULOSE TO GLUCOSE. A STABLE CELLULASE ENZYME OBTAINED FROM A CULTURE OF TRICHODERMA VIRIDE QM 9123 IS COMBINED WITH CELLULOSE TO FORM A SUSPENSION HAVING AT LEAST 100 PARTS BY WEIGHT OF CELLULOSE TO EACH PART BY WEIGHT OF CRUDE ENZYME PROTEIN. THE CELLULOSE-CELLULASE SUSPENSION IS MAINTAINED AT A PH OF FROM 4.0 TO 5.0 AND THE TEMPERATURE IS MAINTAINED WITHIN THE RANGE OF FROM 25* TO 50*C. DURING HYDROLYSIS. UNDER THESE CONDITIONS AND WITH THE CONTINUOUS ADDITION OF FRESH CELLULOSE TO MAINTAIN THE ABOVE RATIO OF CELLULOSE TO CELLULASE, THE CELLULASE ENZYME IS EFFECTIVELY IMMOBILIZED BY ADSORPTION ON UNHYDROLYZED CELLULOSE, ALLOWING THE REMOVAL OF THE SOLUBLE SUGARS BY SIMPLE SEPARATION PROCEDURES WHILE RETAINING MOST OF THE OTHERWISE SOLUBLE ENZYME MATERIAL.
Inventors
- 👤 M Mandels
- 👤 J Kostick
Bibliographic Details
Claims Analysis
Total Claims: 0References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-08 | 1977-04-09 | 1977-10-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-08 | 1981-04-09 | 1981-10-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-08 | 1985-04-09 | 1985-10-09 | $7700.00 | Window Closed / Lapsed |