One step chromatographic isolation of orgotein
Abstract
Abstract SUBSTANTIALLY PURE ORGOTEIN IS OBTAINED IN A SINGLE CHROMATOGRAPHIC PURIFICATION STEP FROM THE MIXTURE OF SOLUBLE PROTEINS PRESENT IN BEEF LIVER AND OTHER ANIMAL TISSUES BY CHROMATOGRAPHING AN AQUEOUS SOLUITION OF THE MIXTURE OF PROTEINS HAVING A PH OF ABOUT 6 AND AN IONIC STREGTH BELOW ABOUT 0.01 OVER A DEAE-CELLULOSE OR OTHER WEAKLY BASIC ION EXCHANGE COLUMN; ELUTING A PORTION OF THE ADSORBED PROTEINS FROM THE COLUMN WITH AQUEOUS ELUANT OF IONIC STRENGTH BELOW ABOUT 0.02 M UNTIL A DROP IN 265 AND 280 NM. ABSORBANCES IS ACCOMPANIED BY A RISE IN DIVALENT METAL ION CONTENT; SELECTIVELY ELUTING THE ADSORBED ORGOTEIN WITH AQUEOUS ELUANT OF A HIGHER IONIC STRENGTH FROM ABOUT 0.02 TO 0.03 M IONIC STRENGTH WHILE MEASURING THE DIVALENT METAL CONTENT OF THE ELUATE; AND SEPARATING ORGOTEIN FROM THAT PORTION OF THE ELUATE HAVING THE MAXIMUM DIVALENT METAL CONTENT.
Inventors
- 👤 W Huber
- 👤 S Chow
- 👤 M Saifer
Bibliographic Details
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