US3763000A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzymatic production of cephalexin
Assignee: Toyo Jozo KK
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Filing: 1972-08-21
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Granted: 1973-10-02
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Expires: 1992-08-21
Abstract
Abstract Cephalexin is produced from 7-amino desacetoxy cephalosporanic acid and phenylglycine ester by enzymatic acylation with acylating enzyme derived from a microorganism selected from the group consisting of genus Alcaligenes, genus Achromobacter, genus Flavobacter, genus Bacillus and genus Beneckea.
Inventors
- 👤 J Abe
- 👤 T Watanabe
- 👤 T Yamaguchi
- 👤 Y Shibuya
- 👤 K Hanamitsu
- 👤 T Fujii
- 👤 K Matsumoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 12:04
CPC Classifications
C; C12; C12P; C12P35/00; C12P35/04; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/824; Y10S435/829; Y10S435/832; Y10S435/85
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
38 words
A process according to claim 1 wherein said micro-organism is a strain selected from the group consisting of Alcaligenes faecalis ATCC 8750, Achromobacter NRRL B-5393, Flavobacterium aquatile NRRL B-5394, Bacillus megaterium NRRL B-5385 and Beneckea hyperoptica ATCC 15803.
Claim 2
Dependent Claim
51 words
A process according to claim 1 wherein the acylating enzyme is present in a preparation selected from the group consisting of microbial cells of a strain of Alcaligenes faecalis ATCC 8750, Achromobacter NRRL B-5393, Flavobacterium aquatile NRRL B-5394, Bacillus megaterium NRRL B-5385 or Beneckea hyperoptica ATCC 15803, and cultured broth thereof.
Claim 3
Dependent Claim
33 words
A process according to claim 1 wherein the derivative of D-phenylglycine is a compound selected from the group consisting of D-phenylglycine methyl ester, D-phenylglycine ethyl ester, D-phenylglycine amide, D-phenylglycyl glycine and D-phenylglycyl leucine.
Claim 4
Dependent Claim
15 words
A process according to claim 1 wherein the acylating enzyme is an insolubilized enzyme preparation.
Claim 5
Dependent Claim
58 words
A process according to claim 5 wherein the insolubilized enzyme preparation is a carrier adsorbed with cultured broth of a microorganism selected from the group consisting of Alcaligenes faecalis ATCC 8750, Achromobacter NRRL B-5393, Flavobacterium aquatile NRRL B-5394, Bacillus megaterium NRRL B-5385 and Beneckea hyperoptica ATCC 15803, with microbial cells thereof or with the acylating enzyme prepared therefrom.
Claim 6
Dependent Claim
34 words
A process according to claim 6 wherein the carrier is selected from the group consisting of diatomaceous earth, acid clay, active clay, alumina, kaolin, calcium phosphate, hydroxyapatite, cellulose and carboxy methyl cross linked dextran.
Claim 7
Dependent Claim
47 words
A process according to claim 1 wherein the reaction is carried out at 20*-45* C., at pH 5.5-7.5, for 0.5-3 hours, with a concentration of 7-amino cephalosporanic acid of 0.1-20 mg./ml., and a concentration of D-phenylglycine ester in 2-20 molar excess relative to the 7-amino cephalosporanic acid.
Claim 8
Dependent Claim
29 words
A process according to claim 8 wherein the reaction is carried out at 30*-37* C., at pH 6.0-6.5, said concentration being 2-10 mg./ml. and said molar excess being 2-10.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-01 | 1977-04-02 | 1977-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-01 | 1981-04-02 | 1981-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-01 | 1985-04-02 | 1985-10-02 | $7700.00 | Window Closed / Lapsed |