US3755132A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluid belt electrophoresis apparatus
Assignee: University of California
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Filing: 1971-11-08
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Granted: 1973-08-28
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Expires: 1991-11-08
Abstract
Abstract A stabilized electrophoretic apparatus is provided for free continuous flow electrophoresis for separating fractions of a chemical or biological substance, and for the injection and measuring the mobilities thereof. The apparatus provides for theinjection of the material to be processed into a fluid belt of an electrolyte buffer solution to form a streak, with the fluid belt on sheath and the streak being moved in a particular direction by the combined reaction of electric current flowing through the buffer solution and a magnetic field. An electric field is provided substantially perpendicular to the direction of movement of the fluid belt, and this field causes electrophoretic migration of the charged particle components of the streak, thereby defining them from their normal course. The apparatus is constructed to incorporate a window so that an optical path may be established through the fluid belt, so that the electrophoretic mobilities of the charged particles may be readily observed and measured. This optical path also provides for a servo control of the apparatus, as will be described.
Inventors
- 👤 A Kolin
- 👤 S Luner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 03:55
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44769; G01N27/44786
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
165 words
Electrophoretic apparatus comprising: a vessel containing an electrolyte solution in a prescribed configuration; means for introducing a solution or suspension of particles into said electrolyte solution; means for moving said electrolyte solution relative to the point of introduction of said solution or particle suspension generating a streak-like path of said introduced solution or suspension; electrode means for producing an electric field substantially transverse to said streak-like path causing electrophoretic migration of said introduced material in the direction of said electric field, said streak-like path being deflected transversely to the direction of movement of said electrolyte solution by said electric field; said electrode means being located in electrolyte solution continuous with and in electric and hydraulic contact unobstructed by intervening membranes with the electrolyte solution in the region of the streak-like path; means for inducing a flow of said electrolyte solution past said electrode means in a direction away from the region of the streak-like paths to carry away electrolysis products generated by said electrode means.
Claim 2
Dependent Claim
165 words
Electrophoretic apparatus comprising: a vessel containing an electrolyte solution in a prescribed configuration; means for introducing a solution or suspension of particles into said electrolyte solution; means for moving said electrolyte solution relative to the point of introduction of said solution or particle suspension generating a streak-like path of said introduced solution or suspension; electrode means for producing an electric field substantially transverse to said streak-like path causing electrophoretic migration of said introduced material in the direction of said electric field, said streak-like path being deflected transversely to the direction of movement of said electrolyte solution by said electric field; said electrode means being located in electrolyte solution continuous with and in electric and hydraulic contact unobstructed by intervening membranes with the electrolyte solution in the region of the streak-like path; means for inducing a flow of said electrolyte solution past said electrode means in a direction away from the region of the streak-like paths to carry away electrolysis products generated by said electrode means.
Claim 3
Dependent Claim
20 words
The apparatus defined in claim 1, and which includes window means for providing an optical path through said electrolyte solution.
Claim 4
Dependent Claim
31 words
The apparatus defined in claim 7, and which includes window means disposed in said mantle and in said core to provide an optical path through the endless belt of electrolyte solution.
Claim 5
Dependent Claim
16 words
The apparatus defined in claim 2, in which said window means in said mantle are removable.
Claim 6
Dependent Claim
25 words
The apparatus as defined in claim 2, in which said window means are composed of a material to permit the passage of ultraviolet light therethrough.
Claim 7
Dependent Claim
16 words
The apparatus defined in claim 1 in which said prescribed configuration is an endless belt-like configuration.
Claim 8
Dependent Claim
33 words
The apparatus defined in claim 1, and which includes a ferro magnetic core, and a mantle surrounding said core and causing said electrolyte solution to flow as an endless belt around said core.
Claim 9
Dependent Claim
33 words
The apparatus defined In claim 7, and which includes magnetic means associated with said core for creating a magnetic field in a direction to cause the buffer solution to flow around said core.
Claim 10
Dependent Claim
70 words
The apparatus defined in claim 1 in which said last-named means includes first and second reservoirs for the electrolyte solution on opposite sides of the path of the moving electrolyte solution containing streaks of introduced material, and means for introducing electrolyte solution into and removing electrolyte solution from said reservoirs so as to control the component of flow of said moving electrolyte solution along the direction of the electric field.
Claim 11
Dependent Claim
68 words
The apparatus defined in claim 9, and which includes a servo-control system for controlling the flow into or out from said reservoirs so as to control the component of flow of said electrolyte solution along the direction of said electric field so as to insure at all times the passage of at, least one streak through a predetermined point remote from the point of introduction of said particles.
Claim 12
Dependent Claim
54 words
The apparatus defined in claim 10, and which includes: means for directing at least one light beam along an optical path through said electrolyte to be selectively intercepted by a streak in said electrolyte solution, and photo cell means responsive to said light beam and to the interception thereof for controlling said servo-control system.
Claim 13
Dependent Claim
19 words
The apparatus defined in claim 1, in which said electrode means produces an electric field which is periodically reversed.
Claim 14
Dependent Claim
35 words
The apparatus defined in claim 1 wherein said vessel includes: an encompassing mantle and an interiorly located core forming an endless belt of electrolyte solution in which said moving of said electrolyte solution is effected.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-08-27 | 1977-02-26 | 1977-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-08-27 | 1981-02-26 | 1981-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-08-27 | 1985-02-26 | 1985-08-28 | $7700.00 | Window Closed / Lapsed |