US3754862A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reagnet composition and method for determining total bilirubin
Assignee: Roche Diagnostics GmbH
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Filing: 1972-02-29
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Granted: 1973-08-28
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Expires: 1992-02-29
Abstract
Abstract Total bilirubin is determined with a stable, accurate, widerange reagent composition comprising: A. AT LEAST ONE OF THE GROUP OF CHLOROPHENYL DIAZONIUM CHLORIDES CONSISTING OF 2,4-DICHLORO-, 2,5-DICHLORO- OR 2-CHLORO-4-NITROPHENYL-DIAZONIUM CHLORIDE AND, B. AN ALKYL-PHENOL POLYETHYLENE OXIDE; TO PRODUCE A COLOR WHICH IS THEN TAKEN AS A MEASURE OF BILIRUBIN CONTENT.
Inventors
- 👤 A Wahlefeld
- 👤 E Bernt
- 👤 W Gruber
- 👤 H Bergmeyer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 05:47
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/728; Y; Y10; Y10S; Y10S436/00; Y10S436/903; Y10T; Y10T436/00; Y10T436/14; Y10T436/145555; Y10T436/146666
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
17 words
Reagent composition as claimed in claim 1 in which said diazonium chloride is a sOlid diazonium chloride.
Claim 2
Dependent Claim
31 words
Reagent composition as claimed in claim 1 wherein said diazonium chloride is contained in an amount of from 0.3 to 5.0 weight percent based on the weight of alkyl-phenol polyethylene oxide.
Claim 3
Dependent Claim
26 words
Reagent composition as claimed in claim 1 wherein component (b) is an alkyl-phenol polyethylene oxide of from 5 to 15 ethylene oxide moieties in the molecule.
Claim 4
Dependent Claim
14 words
Reagent composition as claimed in claim 1 wherein component (a) is 2,4-dichlorophenyl diazonium chloride.
Claim 5
Dependent Claim
14 words
Reagent composition as claimed in claim 1 wherein component (a) is 2,5-dichlorophenyl diazonium chloride.
Claim 6
Dependent Claim
14 words
Reagent composition as claimed in claim 1 wherein component (a) is 2-chloro-4-nitrophenyl diazonium chloride.
Claim 7
Dependent Claim
22 words
Reagent composition as claimed in claim 1 wherein component (b) is in the form of a solution acidified with a mineral acid.
Claim 8
Dependent Claim
18 words
Reagent composition as claimed in claim 8 wherein the concentration of the acid is 0.01 to 0.2 molar.
Claim 9
Dependent Claim
22 words
Reagent composition as claimed in claim 9 wherein the acidic solution contains from 2 to 10 volume percent of alkyl-phenol polyethylene oxide.
Claim 10
Dependent Claim
69 words
Method for the detection of total bilirubin which method comprises contacting a sample suspected of containing bilirubin with a reagent composition for the determination of total bilirubin, which composition comprises: a. at least one of the group of chlorophenyl diazonium chlorides consisting of 2,4-dichloro-, 2,5-dichloro- and 2-chloro-4-nitro-phenyl-diazonium chloride and, b. an alkyl-phenol polyethylene oxide; and measuring the color produced as a measure of the total bilirubin in the sample.
Claim 11
Dependent Claim
68 words
Method as claimed in claim 11 wherein the said sample is immersed into a solution, in 0.01 to 0.2 molar mineral acid, of an alkyl-phenol polyethylene oxide constituting 2 to 10 volume percent of said solution, and a 2,4-dichloro, 2-5-dichloro- and 2-chloro-4-nitro-phenyl-diazonium chloride constituting 0.03 to 0.1 weight percent of said solution; and then measuring the color produced as a measure of total bilirubin content in the sample.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-08-27 | 1977-02-26 | 1977-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-08-27 | 1981-02-26 | 1981-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-08-27 | 1985-02-26 | 1985-08-28 | $7700.00 | Window Closed / Lapsed |