US3753655A
Expired / Lapsed
US
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Process for isolation and separation of thyroid hormones
Assignee: Individual
•
Filing: 1971-11-09
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Granted: 1973-08-21
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Expires: 1991-11-09
Abstract
Abstract A process for isolating and separating the thyroid hormones triiodothyronine and thyroxine from blood serum which comprises first isolating the hormones from the serum by means of a mixed cationic-anionic dextran ion exchanger, and then separating the hormones from each other by the use of an anionic dextran ion exchanger. This procedure permits the satisfactory removal of interfering substances from the serum without decomposing the two thyroid hormones and can be used with advantage on a small scale, for example, in a clinical diagnostic kit.
Inventors
- 👤 B Schreiber
- 👤 G Knapp
- 👤 H Spitzy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 13:52
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; B; B01; B01J; B01J47/00; B01J47/02; B01J47/04; C; C07; C07C; C07C227/00; C07C227/38; C07C227/40; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/825
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
17 words
The process of claim 1, wherein the second ion exchanger consists of an anionic dextran ion exchanger.
Claim 2
Dependent Claim
36 words
The process of claim 1, wherein the two thyroid hormones are separated from each other by the use of an eluting solvent mixture comprising tetrahydrofurfuryl alcohol-water or tetrahydrofuran-water having a pH of from 7.5 to 8.5.
Claim 3
Dependent Claim
75 words
A process for the isolation and separation of the two thyroid hormones triiodothyronine and thyroxine from blood serum which comprises passing the serum through a mixed cationic-anionic dextran ion exchanger, eluting with a tetrahydrofurfuryl alcohol-water or tetrahydrofuran-water mixture, adjusting the pH of the obtained solution to about 7.8 to 8.2 and introducing the solution into a dextran anion exchange column to obtain a solution containing tRiiodothyronine and monoiodotyrosine, and separating the triiodothyronine from said monoiodotyrosine.
Claim 4
Dependent Claim
34 words
The process of claim 4, wherein the triiodothyronine is separated from the monoiodotyronsine by passing the solution obtained from said anion exchange column through a cation exchange column and eluting with a borate buffer.
Claim 5
Dependent Claim
26 words
The process of claim 4, further comprising the step of eluting thyroxine from said mixed cationic-anionic ion exchanger with an acidic tetrahydrofurfuryl alcohol-water or tetrahydrofuran-water mixture.
Claim 6
Dependent Claim
40 words
The process of claim 6, further comprising the steps of incincerating the obtained triiodothyronine and thyroxine with chloric acid and determining the resultant content of liberated iodine as a measure of the triiodothyronine and thyroxine content of the serum, respectively.
Claim 7
Dependent Claim
17 words
The process of claim 7, wherein the iodine content is measured by means of the cerium(IV)-arsenic(III) reaction.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-08-20 | 1977-02-19 | 1977-08-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-08-20 | 1981-02-19 | 1981-08-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-08-20 | 1985-02-19 | 1985-08-21 | $7700.00 | Window Closed / Lapsed |