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US3746869A Expired / Lapsed US ⏱️ 0.0 years remaining

Method of photometrically plotting light scattering objects

Assignee: Carl Zeiss AG
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Filing: 1971-09-28
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Granted: 1973-07-17
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Expires: 1991-09-28
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Abstract

Abstract The invention contemplates an improved method and apparatus for photometrically plotting light scattered by a light-scattering object, as in the plotting of thin-layer chromatograms. Light is irradiated upon one surface of the object, and both the diffusely reflected light and the directly reflected light are simulatenously evaluated, in such manner (a) that base-line variations are minimized and (b) that light-scattering signal attributable to the observed substance is maximized and more readily identified.

Inventors

  • 👤 S Lindstedt
  • 👤 L Treiber
  • 👤 P Stollnberger
  • 👤 W Tausch

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-24 07:19
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/47; G01N21/25; G01N21/255; G01N21/4738; G01N21/474; G01N21/59; G01N21/5907; G01N30/00; G01N30/90; G01N30/95

Claims Analysis

Total Claims: 28
Claim 1
Independent Claim 73 words
The method of photometrically evaluating light-absorbing zones resulting upon the separation of mixtures of substances in thin layers of light-dispersing material, which comprises irradiating light upon one localized area of the material, performing a traverse of said area over one such zone, simultaneously photometrically and additively measuring diffusely reflected light and transmitted light at said area in the course of the traverse, and recording the additive value as a function of traverse position.
Claim 2
Independent Claim 73 words
The method of photometrically evaluating light-absorbing zones resulting upon the separation of mixtures of substances in thin layers of light-dispersing material, which comprises irradiating light upon one localized area of the material, performing a traverse of said area over one such zone, simultaneously photometrically and additively measuring diffusely reflected light and transmitted light at said area in the course of the traverse, and recording the additive value as a function of traverse position.
Claim 3
Dependent Claim 13 words
The method of claim 1, in which the irradiating light is substantially monochromatic.
Claim 4
Independent Claim 92 words
The method of photometrically plotting light scattered by a light-scattering object, which comprises irradiating light upon one surface of the object, simultaneously photometrically measuring diffusely reflected light and transmitted light for a given location on the object, and adding the simultaneously measured values; the given location being one of a succession involved in a transverse of the object, the added values being plotted as a function of traverse position, and the base-line amplitudes of the two measuring signals being caused to assume the same level, prior to adding the simultaneously measured values.
Claim 5
Independent Claim 92 words
The method of photometrically plotting light scattered by a light-scattering object, which comprises irradiating light upon one surface of the object, simultaneously photometrically measuring diffusely reflected light and transmitted light for a given location on the object, and adding the simultaneously measured values; the given location being one of a succession involved in a transverse of the object, the added values being plotted as a function of traverse position, and the base-line amplitudes of the two measuring signals being caused to assume the same level, prior to adding the simultaneously measured values.
Claim 6
Dependent Claim 29 words
The method of claim 3, in which the base-line amplitudes are equalized by reducing the sensitivity of one photomdtric measurement with respect to that of the other photometric measurement.
Claim 7
Dependent Claim 115 words
Apparatus for photometrically plotting light scattered by a light scattering object, comprising a transpardnt support for the object, means for light-irradiating a limited surface area of the object normal to the support; photoelectric-detector means comprising a first detector on one side of the support for responding to diffusely reflected light from said area of the object, and a second detector on the other side of the support for responding simultaneously to directly transmitted light at said local area; means adding the outputs of said detectors, means for transversing said detector means and said support with respect to each other, and recorder means synchronized with traverse displacement and responsive to the added outputs of said detectors.
Claim 8
Dependent Claim 115 words
Apparatus for photometrically plotting light scattered by a light scattering object, comprising a transpardnt support for the object, means for light-irradiating a limited surface area of the object normal to the support; photoelectric-detector means comprising a first detector on one side of the support for responding to diffusely reflected light from said area of the object, and a second detector on the other side of the support for responding simultaneously to directly transmitted light at said local area; means adding the outputs of said detectors, means for transversing said detector means and said support with respect to each other, and recorder means synchronized with traverse displacement and responsive to the added outputs of said detectors.
Claim 9
Dependent Claim 19 words
Apparatus according to claim 5, in which separate signal amplifiers are interposed between each detector and said adding meAns.
Claim 10
Dependent Claim 37 words
Apparatus according to claim 6, in which at least one of said amplifiers is selectively variable, whereby base-line amplitudes of the amplifier outputs may be caused to assume the same level for supply to said adding means.
Claim 11
Dependent Claim 13 words
Apparatus according to claim 6, in which both said amplifiers are selectively variable.
Claim 12
Dependent Claim 13 words
Apparatus according to claim 5, in which said light-irradiating means includes a monochromator.
Claim 13
Dependent Claim 29 words
Apparatus according to claim 5, in which said detectors are photometer balls disposed above and below said support, in vertically aligned relation, each photometer ball including a photoelectric cell.
Claim 14
Dependent Claim 29 words
Apparatus according to claim 5, in which said detector means includes fiber-optical means disposed on said one side of said support for conducting reflected light to said first detector.
Claim 15
Dependent Claim 29 words
Apparatus according to claim 5, in which said detector means includes fiber-optical means disposed on said other side of said support for conducting transmitted light to said second detector.
Claim 16
Dependent Claim 28 words
Apparatus according to claim 5, in which said light-irradiating means includes a source of light and fiber-optical means between said source and said one side of said support.
Claim 17
Dependent Claim 105 words
Apparatus for photometrically plotting light scattered by a light-scattering object, comprising a transparent support for the object, means for light-irradiating the object normal to the support, and detection means comprising fiber-optical means having a first fiber-optical bundle with ends adjacent one side of the support for responding to diffusely reflected light from a local area of the object, said fiber-optical means having a second fiber-optical bundle with ends adjacent the other side of the support for responding to directly transmitted light at said local area; and means including photoelectric means responsive to the sum of the light components transmitted by said first and second bundles.
Claim 18
Dependent Claim 105 words
Apparatus for photometrically plotting light scattered by a light-scattering object, comprising a transparent support for the object, means for light-irradiating the object normal to the support, and detection means comprising fiber-optical means having a first fiber-optical bundle with ends adjacent one side of the support for responding to diffusely reflected light from a local area of the object, said fiber-optical means having a second fiber-optical bundle with ends adjacent the other side of the support for responding to directly transmitted light at said local area; and means including photoelectric means responsive to the sum of the light components transmitted by said first and second bundles.
Claim 19
Dependent Claim 32 words
Apparatus according to claim 14, in which said light-irradiating means includes a further fiber-optical bundle with ends on said one side of said support and terminating normal thereto at said local area.
Claim 20
Dependent Claim 15 words
Apparatus according to claim 14, in which said last-defined means is a single photoelectric cell.
Claim 21
Dependent Claim 108 words
Method for the photometric evaluation of an object having zones resulting upon the separation of mixtures of substances in thin layers of light-dispersing material, characterized by the fact that the diffusely reflected and the transmitted radiation are photometrically measured simultaneously at the same localized area of the object to be evaluated, that the localized area and the object are traversed with respect to each other to develop the simultaneous radiations as a function of traverse position, and that the two radiations thus obtained are added, the proportion of reflected-to-transmitted radiation thus added being adjusted to substantially equalize the base lines for the respective reflected and transmitted radiation functions.
Claim 22
Dependent Claim 108 words
Method for the photometric evaluation of an object having zones resulting upon the separation of mixtures of substances in thin layers of light-dispersing material, characterized by the fact that the diffusely reflected and the transmitted radiation are photometrically measured simultaneously at the same localized area of the object to be evaluated, that the localized area and the object are traversed with respect to each other to develop the simultaneous radiations as a function of traverse position, and that the two radiations thus obtained are added, the proportion of reflected-to-transmitted radiation thus added being adjusted to substantially equalize the base lines for the respective reflected and transmitted radiation functions.
Claim 23
Dependent Claim 108 words
Method for the photometric evaluation of an object having zones resulting upon the separation of mixtures of substances in thin layers of light-dispersing material, characterized by the fact that the diffusely reflected and the transmitted radiation are photometrically measured simultaneously at the same localized area of the object to be evaluated, that the localized area and the object are traversed with respect to each other to develop the simultaneous radiations as a function of traverse position, and that the two radiations thus obtained are added, the proportion of reflected-to-transmitted radiation thus added being adjusted to substantially equalize the base lines for the respective reflected and transmitted radiation functions.
Claim 24
Dependent Claim 108 words
Method for the photometric evaluation of an object having zones resulting upon the separation of mixtures of substances in thin layers of light-dispersing material, characterized by the fact that the diffusely reflected and the transmitted radiation are photometrically measured simultaneously at the same localized area of the object to be evaluated, that the localized area and the object are traversed with respect to each other to develop the simultaneous radiations as a function of traverse position, and that the two radiations thus obtained are added, the proportion of reflected-to-transmitted radiation thus added being adjusted to substantially equalize the base lines for the respective reflected and transmitted radiation functions.
Claim 25
Independent Claim 66 words
The method of photometrically plotting a thin layer of light-scattering material which comprises at different positions zones of absorbing materials, consisting of the steps of scanning said layer by a light beam, measuring simultaneously the diffusely reflected and the transmitted intensity of said light beam, adding these simultaneously measured values, and plotting the resulting sum-signal as a function of the position of said scanning light beam.
Claim 26
Independent Claim 66 words
The method of photometrically plotting a thin layer of light-scattering material which comprises at different positions zones of absorbing materials, consisting of the steps of scanning said layer by a light beam, measuring simultaneously the diffusely reflected and the transmitted intensity of said light beam, adding these simultaneously measured values, and plotting the resulting sum-signal as a function of the position of said scanning light beam.
Claim 27
Independent Claim 66 words
The method of photometrically plotting a thin layer of light-scattering material which comprises at different positions zones of absorbing materials, consisting of the steps of scanning said layer by a light beam, measuring simultaneously the diffusely reflected and the transmitted intensity of said light beam, adding these simultaneously measured values, and plotting the resulting sum-signal as a function of the position of said scanning light beam.
Claim 28
Independent Claim 66 words
The method of photometrically plotting a thin layer of light-scattering material which comprises at different positions zones of absorbing materials, consisting of the steps of scanning said layer by a light beam, measuring simultaneously the diffusely reflected and the transmitted intensity of said light beam, adding these simultaneously measured values, and plotting the resulting sum-signal as a function of the position of said scanning light beam.

References Cited (Backward Citations)

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USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1976-07-16 1977-01-15 1977-07-17 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1980-07-16 1981-01-15 1981-07-17 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1984-07-16 1985-01-15 1985-07-17 $7700.00 Window Closed / Lapsed

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