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US3741876A Expired / Lapsed US ⏱️ 0.0 years remaining

Method of measuring enzyme reaction rates

Assignee: TSI Mason Research Institute Inc
•
Filing: 1970-08-06
•
Granted: 1973-06-26
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Expires: 1990-08-06
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Abstract

Abstract A REACTION RATE BETWEEN AN ENZYME REACTANT AND A SUBSTRATE REACTANT IS FLUROMETRICALLY MEASURED BY FORMING A SOLID REACTANT FILM OF ONE OF THE REACTANTS ON A SILICONE MATRIX PAD. THE REACTANT FILM IS THEN CONTACTED WITH A SOLUTIONS OF THE OTHER REACTANT TO PRODUCE A FLUORESCENT MATERIAL. THE CHANGE OF FLUORESCENCE WITH TIME IS MEASURED BY PLACING THE PAD IN A FLUROMETER AND THIS RATE OF CHANGE IS COMPARED AGAINST A CALIBRATION CURVE TO DETERMINE THE CONCENTRATION OF THE REACTANT IN THE REACTANT SOLUTION.

Inventors

  • 👤 G Guilbault
  • 👤 R Sanders
  • 👤 R Zimmerman

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 14:49
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; C; C12; C12Q; C12Q1/00

Claims Analysis

Total Claims: 0
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References Cited (Backward Citations)

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USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1976-06-25 1976-12-25 1977-06-26 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1980-06-25 1980-12-25 1981-06-26 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1984-06-25 1984-12-25 1985-06-26 $7700.00 Window Closed / Lapsed

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