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US3732077A Expired / Lapsed US ⏱️ 0.0 years remaining

Method and composition for determining bun

Assignee: Damon Corp
•
Filing: 1971-10-29
•
Granted: 1973-05-08
•
Expires: 1991-10-29
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Abstract

Abstract Blood serum is tested for the presence of urea nitrogen by reacting urea present in the serum with an aqueous reagent having a pH of between 0.4 and 1.5 and containing diacetylmonoxime, phenazone, arsenic pentoxide and sulfuric acid. Reaction is effected by heating the serum-reagent mixture to 85* to 100* C., cooling and measuring the optical absorbance of the cooled mixture at a wavelength between 450 and 485 nm.

Inventors

  • 👤 L Foster
  • 👤 J Hochholzer

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-31 01:18
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/62; Y; Y10; Y10T; Y10T436/00; Y10T436/17; Y10T436/171538

Claims Analysis

Total Claims: 2
Claim 1
Independent Claim 65 words
The method of determining the presence of urea nitrogen in blood serum which comprises mixing a blood serum sample with the reagent of claim 1, heating the mixture between 85* and 100* C., cooling the mixture to room temperature and determining the optical density of the cooled mixture at a wavelength between 450 and 485 nm within about 15 minutes after the mixture is cooled.
Claim 2
Dependent Claim 16 words
The method of claim 2 wherein the pH of the reagent is between 0.4 and 1.5.

References Cited (Backward Citations)

US3511611A Cited
US3567374A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1976-05-07 1976-11-06 1977-05-08 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1980-05-07 1980-11-06 1981-05-08 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1984-05-07 1984-11-06 1985-05-08 $7700.00 Window Closed / Lapsed

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