US3730843A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Mass screening test for serum trypsin inhibitors
Assignee: Warner Lambert Co LLC
•
Filing: 1970-12-14
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Granted: 1973-05-01
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Expires: 1990-12-14
Abstract
Abstract A diagnostic test package for use in an improved semiquantitative method for estimating antitrypsin levels in human blood.
Inventors
- 👤 J Mckie
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 15:39
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; B; B06; B06B; B06B1/00; B06B1/18; B06B1/183; C12Q2337/00; C12Q2337/10; C12Q2337/12; G; G01; G01N; G01N2333/00; G01N2333/81; G01N2333/8107; G01N2333/811; G01N2333/8121; G01N2333/8125; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/976
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
43 words
A diagnostic test procedure as set forth in claim 1, wherein a strip of unexposed and developed color film and the following bottled reagent are utilized: A. freeze-dried trypsin of variable mass to provide on reconstitution with 0.2 ml. of pH 8 buffer:
Claim 2
Dependent Claim
16 words
a vial containing 0.9 mg/ml ( + or - 10%) trypsin (the heterozygous reference control serum),
Claim 3
Dependent Claim
42 words
a vial containing 1.3 mg/ml ( + or - 10%) trypsin (the homozygous reference control serum)and B. freeze-dried trypsin test vials each containing 0.30 mg. of trypsin, and C. 5.0 ml of (0.05-0.10 M) tris, (0.005-0.02 M) CaCl2, pH 8 buffer solution.
Claim 4
Dependent Claim
22 words
In a diagnostic test procedure as set forth in claim 2 wherein said strip of color film is a positive color film.
Claim 5
Dependent Claim
39 words
In a diagnostic test procedure as set forth in claim 1 wherein said color film includes three layers having different colorations and said color changes are produced as a result of the depth of hydrolysis by said residual trypsin.
Claim 6
Dependent Claim
20 words
In a diagnostic test procedure as set forth in claim 4 wherein said color film is a positive color film.
Claim 7
Dependent Claim
37 words
In a diagnostic procedure as set forth in claim 4 wherein a small amount of said mixture is applied to said color film and the time of contact being limited so that said colored end results occur.
Claim 8
Dependent Claim
26 words
In a diagnostic procedure as set forth in claim 6 wherein said color film to which said mixture is applied is washed after a predetermined time.
Claim 9
Dependent Claim
17 words
In a diagnostic procedure as set forth in claim 7 wherein said washed film strip is dried.
Claim 10
Dependent Claim
22 words
In a diagnostic procedure as set forth in claim 7 wherein said film strip is washed in a pH3 water rinse bath.
Claim 11
Dependent Claim
23 words
In a diagnostic procedure as set forth in claim 6 wherein said time of contact is about 5 minutes at about room temperature.
Claim 12
Dependent Claim
18 words
In a diagnostic procedure as set forth in claim 10 wherein a drop of said mixture is applied.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-04-30 | 1976-10-30 | 1977-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-04-30 | 1980-10-30 | 1981-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-04-30 | 1984-10-30 | 1985-05-01 | $7700.00 | Window Closed / Lapsed |