Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
103 words
A method for determining the Zeta Potential of suspended colloidal particles comprising the steps of: a. establishing a predetermined voltage gradient along an electrophoresis cell positioned on a microscope stage, b. introducing a sample of said suspended colloidal particles into said cell, c. scanning a microscopic image of said colloidal particles within said cell, d. tracking said particles to determine their movement, e. storing data representative of said particle movement; f. calculating the particle velocity from said data, and g. determining the Zeta potential from said particle velocity, the voltage gradient of said cell, and the temperature of said suspended colloidal particles sample.
Claim 2
Independent Claim
103 words
A method for determining the Zeta Potential of suspended colloidal particles comprising the steps of: a. establishing a predetermined voltage gradient along an electrophoresis cell positioned on a microscope stage, b. introducing a sample of said suspended colloidal particles into said cell, c. scanning a microscopic image of said colloidal particles within said cell, d. tracking said particles to determine their movement, e. storing data representative of said particle movement; f. calculating the particle velocity from said data, and g. determining the Zeta potential from said particle velocity, the voltage gradient of said cell, and the temperature of said suspended colloidal particles sample.
Claim 3
Dependent Claim
37 words
A method as in claim 1 wherein said particle velocity is determined from the total distance travelled by the individual particles and dividing said total distance by the number of particles observed during a fixed observation period.
Claim 4
Dependent Claim
38 words
The method as in claim 1 wherein said particle velocity is determined by differentiating a signal representative of said particLe displacement with respect to time to provide a signal proportional to the rate of change of particle displacement.
Claim 5
Dependent Claim
73 words
A method as in claim 1 wherein the Zeta Potential is determined from the following formula: ZP -K1 (Ve/V) (1.45 - 0.02tc) and wherein K1 4 pi Ln/D, Ve is the electrophoretic velocity of said particles, V is the voltage across said cell, tc is the temperature of said sample, L is the length of said cell, D is the dielectric constant of said sample, and n is the viscosity of said sample.
Claim 6
Dependent Claim
82 words
A method as in claim 1 wherein the Zeta Potential is determined from the following formula: ZP -K2 (d/V) (1.45 - 0.02tc) wherein K2 4 pi Ln/D, d is the distance travelled during a predetermined observation interval, V is the voltage of said cell, tc is the temperature of said sample, L is the length of said cell, n is the viscosity of said sample and D is the dielectric constant of said sample and where K2 includes said predetermined observation interval.
Claim 7
Dependent Claim
85 words
A method as in claim 1 wherein the Zeta Potential is determined from the following formula: ZP (K3/TV) (1.45 - 0.02tc) wherein -K3 4 pi Ln/D, T is the average time required for the particles to travel a predetermined distance, V is the voltage across said cell, and tc is the temperature of said sample, L is the length of said cell, n is the viscosity of said sample and D is the dielectric constant of said sample and wherein K3 includes said predetermined distance.