US3721725A
Expired / Lapsed
US
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Process for preparing powder blends
Assignee: EIDP Inc
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Filing: 1970-08-14
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Granted: 1973-03-20
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Expires: 1990-08-14
Abstract
Abstract A method of making a homogeneous solid particulate blend of solid initial ingredients involves dissolving in an aqueous solvent a sugar that is sublimable to dryness, a lesser amount of a biological product, and, if the biological product is not sufficiently lubricative, a lubricant; spraying this solution into boiling dichlorodifluoromethane or other fluorocarbon refrigerant; and lyophilizing the resulting frozen droplets. The sugar is usually either mannitol, maltose, lactose, inositol or dextran, or combinations of these sugars. Other sugars in an amount less than the above sugars may also be added to the solution. Less than about 5 percent w/v of an electrolyte may beneficially be added to the solution, as well as other substances, such as acid, base, etc. Ultrasonic waves applied to the fluorocarbon refrigerant in the region where the solution is sprayed increases the freezing rate. Limiting the distribution of sizes of those particles to be tabletted increases the weight homogeneity of the resulting tablets.
Inventors
- 👤 A Briggs
- 👤 T Maxwell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 10:21
CPC Classifications
F; F26; F26B; F26B5/00; F26B5/04; F26B5/06; F26B5/065; A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/128; A61; A61K; A61K9/00; A61K9/14; A61K9/19; A61K9/20; A61K9/2095; A61L; A61L2/00; A61L2/02; C; C12; C12N; C12N9/00; C12N9/98; F25; F25C; F25C1/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/96
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
33 words
The method of claim 1 wherein said sugar is at least one selected from the group consisting of mannitol, maltose, lactose, inositol, and dextran, and said solid material is a solid biological product.
Claim 2
Dependent Claim
30 words
The method of claim 2 wherein said solid biological product is selected from the group consisting of luciferin, bovine albumin, morpholinopropane sulfonic acid, 2,6-dichlorophenol indophenol, nicotinamide-adenine-dinucleotide (reduced), and nicotin amide-adenine-dinucleotide.
Claim 3
Dependent Claim
28 words
The method of claim 2 wherein said fluorocarbon refrigerant is selected from the group consisting of dichlorodifluoromethane, trichlorofluoromethane, chlorotrifluoromethane, tetrafluoromethane, dichlorofluoromethane, chlorodifluoromethane, trifluoromethane, dichlorotetrafluoroethane, chloropentafluoroethane, bromotrifluoromethane and dibromotetrafluoroethane.
Claim 4
Dependent Claim
11 words
The method of claim 4 wherein said fluorocarbon refrigerant is dichlorodifluoromethane.
Claim 5
Dependent Claim
26 words
The method of claim 2 wherein a water-soluble lubricant is included in said solution in a concentration less than about 10 percent w/v of said solution.
Claim 6
Dependent Claim
46 words
The method of claim 6 wherein, prior to the spraying step, an amount of an electrolyte compatible with said biological product, said sugar, and said solvent is dissolved in said solution, said amount of said electrolyte being less than about 5 percent w/v of said solution.
Claim 7
Dependent Claim
21 words
The method of claim 7 wherein said electrolyte is selected from the group consisting of KCl, NaCl, MgSo4, MgCl2, and (NH4)2SO4.
Claim 8
Dependent Claim
64 words
The method of claim 6 wherein several predetermined portions, having similar weight to each other, of particles are removed and formed into tablets, and wherein the diameter of said particles in said portions are a. substantially all greater than 0.01 inch; and b. within a range such that the coefficient of variation of the weight of said tablets is less than about 1 percent.
Claim 9
Dependent Claim
16 words
The method of claim 9 wherein substantially all of said diameters are less than 0.017 inch.
Claim 10
Dependent Claim
27 words
The method of claim 6 wherein said biological product is an enzyme selected from the group consisting of luciferase, hexokinase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase, and pyruvate kinase.
Claim 11
Dependent Claim
19 words
The method of claim 11 wherein said water-soluble lubricant is selected from the group consisting of carbowax and leucine.
Claim 12
Dependent Claim
61 words
The method of claim 11 wherein said sugar and said lubricant are dissolved in said aqueous solvent prior to dissolving said biological product and, prior to dissolving said biological product, a pH-controlling substance selected from the group consisting of acids, bases and buffers is added to said solution to adjust to pH to within a range of about 3 to 10.
Claim 13
Dependent Claim
80 words
The method of claim 13 wherein: a. prior to adding said pH-controlling substance, an amount of an electrolyte selected from the group consisting of KCl, NaCl, MgSo4, MgCl2, and (NH4)2SO4 is dissolved in said solvent, said amount being less than 5 percent w/v of said solution; b. said lubricant is selected from the group consisting of carbowax and leucine; and c. said biological product is selected from the group consisting of luciferase, hexokinase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase and pyruvate kinase.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-03-19 | 1976-09-18 | 1977-03-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-03-19 | 1980-09-18 | 1981-03-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-03-19 | 1984-09-18 | 1985-03-20 | $7700.00 | Window Closed / Lapsed |