US3717436A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the sequential degradation of peptide chains
Assignee: Beckman Coulter Inc
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Filing: 1971-01-25
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Granted: 1973-02-20
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Expires: 1991-01-25
Abstract
Abstract An improvement in the process of sequentially degrading peptide chains in a reaction vessel by successive coupling and cleavage reaction steps, wherein the vessel is subjected to a vacuum purging operation between the reaction steps to remove undesired materials. The improvement involves flushing the vessel with a stream of nitrogen gas while it is under vacuum whereby the undesired materials are more rapidly and more completely removed from the vessel and the cumulative yield of the process is increased.
Inventors
- 👤 H Penhasi
- 👤 H Meier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 16:47
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6818; G01N33/6824
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
80 words
In the process of sequentially degrading peptide chains in a reaction vessel by successive coupling and cleavage reaction steps, constituting a cycle, wherein the vessel is subjected to a vacuum purging operation between reaction steps to remove undesired materials, the improvement which comprises flushing the vessel with a stream of nitrogen gas while it is under vacuum whereby the undesired materials are more rapidly and more compleTely removed from the vessel and the cumulative yield of the process is increased.
Claim 2
Dependent Claim
80 words
In the process of sequentially degrading peptide chains in a reaction vessel by successive coupling and cleavage reaction steps, constituting a cycle, wherein the vessel is subjected to a vacuum purging operation between reaction steps to remove undesired materials, the improvement which comprises flushing the vessel with a stream of nitrogen gas while it is under vacuum whereby the undesired materials are more rapidly and more compleTely removed from the vessel and the cumulative yield of the process is increased.
Claim 3
Dependent Claim
17 words
The process of claim 1 wherein each reaction cycle takes from about 70 to about 90 minutes.
Claim 4
Dependent Claim
23 words
The process of claim 1 wherein the nitrogen stream passes over substantially the entire surface area of the material in the reaction vessel.
Claim 5
Dependent Claim
32 words
The process of claim 1 wherein the vacuum level is from about 450 to about 550 microns of mercury and the flow rate of nitrogen is about 100 cubic centimeters per minute.
References Cited (Backward Citations)
US3536450A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-02-20 | 1976-08-21 | 1977-02-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-02-20 | 1980-08-21 | 1981-02-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-02-20 | 1984-08-21 | 1985-02-20 | $7700.00 | Window Closed / Lapsed |