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US3716634A Expired / Lapsed US ⏱️ 0.0 years remaining

Insect virus compositions and process

Assignee: Mclaughlin Gormley King Co
•
Filing: 1970-10-19
•
Granted: 1973-02-13
•
Expires: 1990-10-19
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Abstract

Abstract Insect polyhedrosis and granulosis virus particles are liberated from their inclusion bodies and then complexed and precipitated from an aqueous dispersion with a basic protein having an isoelectric point above about pH 8.0 to provide a virus concentrate. The resulting precipitate may be dispersed in an oil to provide a stabilized viral insecticide.

Inventors

  • 👤 F Wells

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-06 11:18
CPC Classifications
A; A01; A01N; A01N63/00; A01N63/40

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 117 words
A process of preparing a stabilized viral insecticide having a high infectivity titer which comprises macerating in the presence of water bodies of an insect infected with a virus of the polyhedrosis or granulosis type to provide an aqueous suspension of virus inclusion bodies and gross insect fragments, separating said fragments from the suspension containing said inclusion bodies, contacting the resulting suspension with an alkaline reacting material for a time sufficient to liberate the virus particles from said inclusion bodies, contacting the resulting alkaline dispersion with a basic protein having an isoelectric point of at least pH 8.0 whereby to precipitate said virus particles as a complex and then dispersing the resulting complex in an insecticide carrier.
Claim 2
Dependent Claim 117 words
A process of preparing a stabilized viral insecticide having a high infectivity titer which comprises macerating in the presence of water bodies of an insect infected with a virus of the polyhedrosis or granulosis type to provide an aqueous suspension of virus inclusion bodies and gross insect fragments, separating said fragments from the suspension containing said inclusion bodies, contacting the resulting suspension with an alkaline reacting material for a time sufficient to liberate the virus particles from said inclusion bodies, contacting the resulting alkaline dispersion with a basic protein having an isoelectric point of at least pH 8.0 whereby to precipitate said virus particles as a complex and then dispersing the resulting complex in an insecticide carrier.
Claim 3
Dependent Claim 11 words
The process of claim 1 wherein said basic protein is lysozyme.
Claim 4
Dependent Claim 20 words
The process of claim 1 wherein said dispersion during contacting has a pH in the range of 8 to 10.
Claim 5
Dependent Claim 14 words
The process of claim 1 wherein said virus is of the nuclear polyhedrosis type.
Claim 6
Dependent Claim 12 words
The process of claim 1 in which the carrier is an oil.
Claim 7
Dependent Claim 12 words
The process of claim 5 wherein the oil is a vegetable oil.

References Cited (Backward Citations)

US3541203A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1976-02-13 1976-08-14 1977-02-13 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1980-02-13 1980-08-14 1981-02-13 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1984-02-13 1984-08-14 1985-02-13 $7700.00 Window Closed / Lapsed

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